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产品概述
产品信息
别名 | B-TCGF, CSIF, TGIF |
物种 | Mouse |
表达宿主 | E.coli |
序列信息 | MSRGQYSREDNNCTHFPVGQSHMLLELRTAFSQVKTFFQTKDQLDNILLTDSLMQDFKGYLGCQALSEMIQFYLVEVMPQAEKHGPEIKEHLNSLGEKLKTLRMRLRRCHRFLPCENKSKAVEQVKSDFNKLQDQGVYKAMNEFDIFINCIEAYMMIKMKS with polyhistidine tag at the C-terminus. |
检索号 | P18893.2 |
分子量 | 19.69 kDa |
表观分子量 | 19 kDa |
标签 | His-tag at the C-terminus |
生物活性 | Measure by its ability to induce MC/9‑2 cells proliferation.The ED50 for this effect is <1 ng/mL.The specific activity of recombinant mouse IL-10 is > 1 x 10^6 IU/mg. |
产品特性
纯度 | >98% as determined by SDS-PAGE. Ni-NTA chromatography |
内毒素 | <0.1 EU per 1 μg of the protein by the LAL method. |
保存 | Lyophilized protein should be stored at -5~-20°C for 1 year. Upon reconstitution, store at 2-8°C for up to 1 week. Further dilute in a buffer containing a carrier protein or stabilizer (e.g.0.1% BSA,10%FBS,5%HSA or 5% trehalose solution), protein aliquots should be stored at -5~-20°C or -80°C for 3-6 months. |
运输 | Ambient temperature or ice pack. |
制剂 | The protein was lyophilized from a 0.2 µm filtered solution containing 1X PBS, pH 7.4. |
复融 | It is recommended to reconstitute the lyophilized protein in sterile water to a concentration not less than 100 μg/mL.Do Not Vortex! Vigorous shaking may impair the biological activity of the protein. |
背景介绍
FAQs
Q:因子到货了,里面有两管溶液,一个是水,一个是海藻糖溶液,用哪一个稀释呢?
A:1、将装有细胞因子的管子放入小离心机内短暂离心20~30s(离心机最大转速即可),使附着在管壁的微量蛋白聚集在管底; 2、先用无菌水溶解因子,很少量水即可溶解因子,注意计算溶解后浓度不低于100 μg/mL; 3、用赠送的含海藻糖的PBS溶液进一步稀释到您实验合适的浓度即可,然后分装后放入-20℃以下保藏; 4、因子复融重悬过程应轻柔,不可以震荡涡旋。
Q:收到细胞因子产品用什么溶剂复融?
A:1、细胞因子产品大部分为冻干形式发货,少部分液体形式发货; 2、液体形式因子产品不需要复溶,具体浓度详见产品标签,按照实验需要用含载体蛋白(如0.1% BSA,10%FBS,5%HSA 或 5% 海藻糖)的溶液进一步稀释后分装冷冻保存即可; 3、冻干粉状态的因子产品需指定溶剂复溶,请参考产品说明书【复融】方法,按照要求使用对应溶剂溶解,部分需要无菌水以外的特殊溶剂溶解的产品则随货会赠送相应的复融试剂;冻干粉溶解后如您需要进一步稀释分装,需使用含载体蛋白(如0.1% BSA,10%FBS,5%HSA 或 5% 海藻糖)的溶液进一步稀释后分装冷冻保存。
产品资料
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