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| 名称 | HCC70 (人乳腺导管癌细胞) (STR鉴定正确) |
| 别称 | HCC-70; HCC 70; HCC0070; Hamon Cancer Center 70 |
| 种属 | 人 |
| 生长特性 | 贴壁细胞 |
| 细胞形态 | 上皮细胞样 |
| 冻存条件 | 冻存液：55% 基础培养基+40%FBS+5%DMSO 温度：液氮 |
| 培养方案A（默认） |
培养条件:
气相：空气，95%；CO2，5%, 温度：37℃
|
| 推荐传代比例 | 1:4-1:6 |
| 推荐换液频率 | 2-3次/周 |
参考资料(来源文献)
| 背景描述 | HCC70 is an epithelial cell line isolated from a primary ductal carcinoma from a 49-year-old, Black female in 1992, and the cells took 44 months to establish. HCC70 is positive for the epithelial cell specific marker Epithelial Glycoprotein 2 (EGP2) and for cytokeratin 19.HCC70 is positive for the epithelial cell specific marker Epithelial Glycoprotein 2 (EGP2) and for cytokeratin 19. However, recent studies performed outside of ATCC have given conflicting results about the status of the estrogen receptor for these cells. The cells are poorly differentiated. The cells overexpress p53, and are negative for the expression of Her2-neu oncogenes. The depositor of CRL-2315 initially reported the cells were positive for estrogen receptors. |
| 年龄（性别） | 女性；49岁 |
| 组织来源 | 乳腺 |
| 细胞类型 | 肿瘤细胞 |
| 肿瘤类型 | 乳腺导管癌 |
| 生物安全等级 | BSL-1 |
| 倍增时间 | 93 hours (PubMed=25984343) |
| 基因表达情况 | epithelial glycoprotein 2 (EGP2); cytokeratin 19 |
| 保藏机构 | ATCC; CRL-2315 |
STR鉴定
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STR位点信息
Amelogenin X CSF1PO 10,14 D2S1338 23,25 D3S1358 16,17 D5S818 12,13 D7S820 10,11 D8S1179 13 D13S317 12 D16S539 9,13 D18S51 13,16 D19S433 12.2,15.2 D21S11 30 FGA 19.2,24 PentaD 10 PentaE 9 TH01 9 TPOX 9,10 vWA 13,15 D6S1043 14,24 D12S391 15,19 D2S441 11 -
STR鉴定图
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