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人子宫内膜上皮细胞
Human Endometrial Epithelial Cells
货号:CP-H058
规格:5×10⁵Cells/T25培养瓶
价格: ¥6880
培养基货号: CM-H058
产品概述
人子宫内膜上皮细胞
Cat NO.: CP-H058
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产品名称:人子宫内膜上皮细胞
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组织来源:子宫组织
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产品规格:5×105cells/T25细胞培养瓶
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细胞简介:
人子宫内膜上皮细胞分离自子宫组织;子宫是孕育胎儿的器官,位于盆腔中部,膀胱与直肠之间,其位置可随膀胱与直肠的充盈程度或体位而有变化。子宫的正常位置主要依靠子宫诸韧带、盆膈、尿生殖膈及会阴中心腱等结构维持,这些结构受损或松弛时,可以引起子宫脱垂。子宫内膜即黏膜,由上皮(属单层柱状上皮,有分泌细胞和纤毛细胞二种)和固有膜(由结缔组织构成,其内有大量的星形细胞,称为基质细胞)组成,子宫内膜可分为浅表的功能膜和深部的基底层,功能层较厚,约占内膜厚度的4/5;基底层较薄较致密,约占1/5,功能层可剥脱,而基底层不可剥脱。子宫内膜上皮细胞主要功能:①子宫内膜亦称子宫黏膜,是指构成哺乳类子宫内壁的一层;②子宫内膜对动情素和孕激素都起反应,因此可随着性周期(发情周期、月经周期)发生显著的变化。子宫内膜与胚胎附植密切相关,在生殖生理的研究中占重要地位。在胚胎与母体“对话”的过程中,子宫内膜上皮细胞充当了极其重要的角色。子宫内膜构成雌性哺乳动物子宫壁的最内层,位于子宫腔面,在动物生殖生理活动中占有重要地位。子宫和子宫内膜是维持雌性动物生理功能和生育能力的重要器官,子宫内膜的再生修复是子宫的重要生理功能。体外培养的子宫内膜上皮细胞对于研究其生理功能、药物作用以及各种致病因素作用下的病理生理改变具重要意义。
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方法简介:
普诺赛实验室分离的人子宫内膜上皮细胞采用胶原酶消化法,结合上皮细胞专用培养基培养筛选制备而来,细胞总量约为5×10⁵cells/瓶。
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质量检测:
普诺赛实验室分离的人子宫内膜上皮细胞经Cytokeratin-19免疫荧光鉴定,纯度可达90%以上,且不含有HIV-1、HBV、HCV、支原体、细菌、酵母和真菌等。
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培养信息:
包被条件 鼠尾胶原Ⅰ(2-5μg/cm2) 培养基 含FBS、生长添加剂、Penicillin、Streptomycin等 产品货号 CM-H058 换液频率 每2-3天换液一次 生长特性 贴壁 细胞形态 上皮细胞样 传代特性 可传2-3代 消化液 0.25%胰蛋白酶 培养条件 气相:空气,95%;CO2,5% 人子宫内膜上皮细胞体外培养周期有限;建议使用普诺赛配套的专用生长培养基及正确的操作方法来培养,以此保证该细胞的最佳培养状态。
参考文献
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The humanin analogue (HNG) alleviates intrauterine adhesions by inhibiting endometrial epithelial cells ferroptosis: a rat model-based study(2023/10/10)
作者:Liping Zou, Jin Huang, Qiaoling Zhang
期刊:HUMAN REPRODUCTION
影响因子 :6.100
引用产品:CP-H058
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Oxidized LDL promotes EMS-induced angiogenesis by increasing VEGF-A expression and secretion by endometrial cells(2022/12/12)
作者:Caiqi Ma, Wei Huang, Hui Wang
期刊:MOLECULAR MEDICINE
DOI:10.1186/s10020-022-00582-6
影响因子 :5.700
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The potential role of methyltransferase-like 5 in deficient mismatch repair of uterine corpus endometrial carcinoma(2022/02/15)
作者:Xiaojuan Liu, Hui Ma, Lisha Ma
期刊:Bioengineered
DOI:10.1080/21655979.2022.2036912
影响因子 :4.900
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Exosomal lncRNA NEAT1 from cancer-associated fibroblasts facilitates endometrial cancer progression via miR-26a/b-5p-mediated STAT3/YKL-40 signaling pathway(2021/06/18)
作者:Jiangtao Fan, Zhaoyu Zhou, Yanlu Luo
期刊:Neoplasia
影响因子 :4.800
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Long non-coding RNA JPX promotes endometrial carcinoma progression via janus kinase 2/signal transducer and activator of transcription 3(2024/05/09)
作者:Hanzhen Xiong, Wei Zhang, Mingyu Xie
期刊:Frontiers in Oncology
影响因子 :4.700
引用产品:CP-H058
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Identification of immune-related gene signature for predicting prognosis in uterine corpus endometrial carcinoma(2023/06/07)
作者:Siyuan Song, Haoqing Gu,Jingzhan Li
期刊:Scientific Reports
DOI:10.1038/s41598-023-35655-x
影响因子 :4.600
引用产品:CP-H058
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Circ_0109046 promotes the malignancy of endometrial carcinoma cells through the microRNA‐105/SOX9/Wnt/β‐catenin axis(2020/11/21)
作者:Yanyan Li, Jinyu Liu, Jinxia Piao
期刊:Iubmb Life
DOI:10.1002/iub.2415
影响因子 :4.600
引用产品:CP-H058
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Long noncoding RNA SNHG25 promotes the malignancy of endometrial cancer by sponging microRNA-497-5p and increasing FASN expression(2021/11/18)
作者:Yuhua He, Shuifang Xu, Yi Qi
期刊:Journal of Ovarian Research
DOI:10.1186/s13048-021-00906-w
影响因子 :4.000
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Exosomal miR-543 derived from umbilical cord mesenchymal stem cells ameliorates endometrial fibrosis in intrauterine adhesion via downregulating N-cadherin(2022/11/26)
作者:Donglan Yuan, Ting Guo, Hua Qian, Chunyan Jin, Hongshan Ge, Yinling Zhao, Dandan Zhu, Mei Lin, Hua Wang, Hong Yu
期刊:PLACENTA
DOI:10.1016/j.placenta.2022.11.013
影响因子 :3.800
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Knockdown of Trop2 inhibits proliferation and migration and induces apoptosis of endometrial cancer cells via AKT/β‐catenin pathway(2020/01/22)
作者:Xiaotong Sun, Guangyang Xing, Cui Zhang
期刊:Cell Biochemistry and Function
DOI:10.1002/cbf.3450
影响因子 :3.600
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MicroRNA-199a/b-5p inhibits endometrial cancer cell metastasis and invasion by targeting FAM83B in the epithelial‑to‑mesenchymal transition signaling pathway(2021/3/2)
作者:Hanzhen Xiong, Na Wang, Hui Chen
期刊:Molecular Medicine Reports
影响因子 :3.400
引用产品:CP-H058
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Human Umbilical Cord Mesenchymal Stem Cell-Derived Conditioned Medium Promotes Human Endometrial Cell Proliferation through Wnt/β-Catenin Signaling(2022/08/30)
作者:Xiaoning Wei, Feiran Liu, Sichen Zhang
期刊:Biomed Research International
影响因子 :3.246
引用产品:CP-H058
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TNFAIP3 interacting protein 2 relieves lipopolysaccharide (LPS)-induced inflammatory injury in endometritis by inhibiting NF-kappaB activation(2023/10/13)
作者:Xinxin Qian, Yan Wang, Xingmei Li
期刊:Immunity Inflammation and Disease
DOI:10.1002/iid3.970
影响因子 :3.200
引用产品:CP-H058
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LncRNA IGF2-AS promotes endometriosis progression through targeting miR-370-3p/IGF2 axis and activating PI3K/AKT/mTOR signaling pathway(2022/12/12)
作者:Xiaoyan Jin, Jingjing Feng, Xiao Cheng
期刊:JOURNAL OF ASSISTED REPRODUCTION AND GENETICS
DOI:10.1007/s10815-022-02638-2
影响因子 :3.100
引用产品:CP-H058
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Unveiling the protective effects of BMSCs/anti-miR-124-3p exosomes on LPS-induced endometrial injury(2024/02/16)
作者:Yihong Chen, Shan Zheng, Xiumei Zhao
期刊:FUNCTIONAL & INTEGRATIVE GENOMICS
DOI:10.1007/s10142-024-01303-4
影响因子 :2.900
引用产品:CP-H058
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A novel necroptosis-associated miRNA signature predicting prognosis of endometrial cancer and correlated with immune infiltration(2023/03/23)
作者:Zhongxue Ye, Yafen Jiang, Junlong Wu
期刊:Taiwanese Journal of Obstetrics & Gynecology
DOI:10.1016/j.tjog.2022.09.009
影响因子 :2.100
引用产品:CP-H058
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