产品中心Cell Resources
联系我们CONTACT US
- 400-999-210024小时服务热线
产品概述
人椎间盘髓核细胞
Cat NO.: CP-H097
1.产品名称:人椎间盘髓核细胞
2.组织来源:椎间盘组织
3.产品规格:5×105cells/T25细胞培养瓶
4.细胞简介:
人椎间盘髓核细胞分离椎间盘组织;椎间盘是位于脊柱两椎体之间,分为中央部的髓核,富于弹性的胶状物质;周围部的纤维环,由多层纤维软骨环按同心圆排列。上下有软骨板,是透明软骨复盖于椎体上,下面骺环中间的骨面。上下的软骨板与纤维环一起将髓核密封起来。纤维环由胶原纤维束的纤维软骨构成,位于髓核的四周。纤维环的纤维束相互斜行交叉重叠,使纤维环成为坚实的组织,能承受较大的弯曲和扭转负荷。髓核,是乳白色半透明胶状体,富于弹性,为椎间盘结构的一部分,位于两软骨板与纤维环之间。由纵横交错的纤维网状结构即软骨细胞和蛋白多糖黏液样基质构成的弹性胶冻物质。婴幼儿时期的髓核含水量为80%-90%,即使到了老年,其含水量也在70%上下。髓核在出生时体积大而松散,位于椎间盘的中央,至成年时位置移至椎间盘的中后部;在成年以前构成髓核的主要物质是大量蛋白多糖复合体、胶原纤维和纤维软骨,随着年龄的增长,髓核中的蛋白多糖解聚增多,水分逐渐减少,胶原增粗并逐渐被纤维软骨所替代。
5.方法简介:
普诺赛实验室分离的人椎间盘髓核细胞采用胶原酶-中性蛋白酶混合消化法并结合软骨细胞专用培养基培养筛选制备而来,细胞总量约为5×10⁵cells/瓶。
6.质量检测:
普诺赛实验室分离的人椎间盘髓核细胞经Ⅱ型胶原蛋白免疫荧光鉴定,纯度可达90%以上,且不含有HIV-1、HBV、HCV、支原体、细菌、酵母和真菌等。
7.培养信息:
包被条件 | |
培养基 | 含FBS、生长添加剂、Penicillin、Streptomycin等 |
产品货号 | CM-H097 |
换液频率 | 每3-4天换液一次 |
生长特性 | 贴壁 |
细胞形态 | 梭形、多角形 |
传代特性 | 可传5代左右;3代以内状态最佳 |
消化液 | 0.25%胰蛋白酶 |
培养条件 | 气相:空气,95%;CO2,5% |
人椎间盘髓核细胞体外培养周期有限;建议使用普诺赛配套的专用生长培养基及正确的操作方法来培养,以此保证该细胞的最佳培养状态。
产品手册
参考文献
-
Pharmacological network analysis of the functions and mechanism of kaempferol from Du Zhong in intervertebral disc degeneration (IDD)(2023/03/03)
作者:Xiaobin Wang, Yanlin Tan, Fusheng Liu
期刊:Journal of Orthopaedic Translation
影响因子 :6.600
引用产品:CP-H097
-
hASCs-derived exosomal miR-155-5p targeting TGFβR2 promotes autophagy and reduces pyroptosis to alleviate intervertebral disc degeneration(2023/03/14)
作者:Dong Chen, Xin Jiang, Haibo Zou
期刊:Journal of Orthopaedic Translation
影响因子 :6.600
-
BSHXF-medicated serum combined with ADSCs regulates the TGF-β1/Smad pathway to repair oxidatively damaged NPCs and its component analysis(2023/06/03)
作者:Jiahao Duan, Zhaoyong Li, Enxu Liu
期刊:JOURNAL OF ETHNOPHARMACOLOGY
影响因子 :5.400
引用产品:CP-H097
-
Exogenous Klotho ameliorates extracellular matrix degradation and angiogenesis in intervertebral disc degeneration via inhibition of the Rac1/PAK1/MMP-2 signaling axis(2022/08/08)
作者:Yuyang Yi, Hao Chen, Shubao Zhang
期刊:MECHANISMS OF AGEING AND DEVELOPMENT
影响因子 :5.300
引用产品:CP-H097
-
microRNA-365 attenuated intervertebral disc degeneration through modulating nucleus pulposus cell apoptosis and extracellular matrix degradation by targeting EFNA3(2023/11/27)
作者:Chao Jiang, Youjun Liu, Weigong Zhao
期刊:JOURNAL OF CELLULAR AND MOLECULAR MEDICINE
影响因子 :5.300
引用产品:CP-H097
-
Lumican silencing alleviates tumor necrosis factor-α-induced nucleus pulposus cell inflammation and senescence by inhibiting apoptosis signal regulating kinase 1/p38 signaling pathway via inactivating Fas ligand expression(2021/9/13)
作者:Zhenqiang Li, Chengfeng Sun, Maosong Chen
期刊:Bioengineered
DOI:10.1080/21655979.2021.1973781
影响因子 :4.900
引用产品:CP-H097
-
The protective effects of dezocine on interleukin-1β-induced inflammation, oxidative stress and apoptosis of human nucleus pulposus cells and the possible mechanisms(2022/01/03)
作者:Fang Zhu, Wei Duan, Chao Zhong
期刊:Bioengineered
DOI:10.1080/21655979.2021.2017700
影响因子 :4.900
引用产品:CP-H097
-
Kukoamine A attenuates lipopolysaccharide-induced apoptosis, extracellular matrix degradation, and inflammation in nucleus pulposus cells by activating the P13K/Akt pathway(2022/03/25)
作者:Dan Wang , Hao Qu , Hui Kang
期刊:Bioengineered
DOI:10.1080/21655979.2022.2051855
影响因子 :4.900
引用产品:CP-H097
-
MicroRNA-137 inhibits the inflammatory response and extracellular matrix degradation in lipopolysaccharide-stimulated human nucleus pulposus cells by targeting activin a receptor type I(2022/03/02)
作者:Bin Yu, Ziqi Zhu, Beiduo Shen
期刊:Bioengineered
DOI:10.1080/21655979.2022.2042987
影响因子 :4.900
引用产品:CP-H097
-
Platelet-rich plasma-derived extracellular vesicles inhibit NF-κB/NLRP3 pathway-mediated pyroptosis in intervertebral disc degeneration via the MALAT1/microRNA-217/SIRT1 axis(2024/02/17)
作者:Xueqiang Tao, Fen Xue, Jiayuan Xu
期刊:CELLULAR SIGNALLING
DOI:10.1016/j.cellsig.2024.111106
影响因子 :4.800
引用产品:CP-H097
-
Micro Fragmented Adipose Tissue Promotes the Matrix Synthesis Function of Nucleus Pulposus Cells and Regenerates Degenerated Intervertebral Disc in a Pig Model:(2020/2/7)
作者:Xiaopeng Zhou, Feng Zhang, Dawei Wang
期刊:Cell Transplantation
影响因子 :3.300
引用产品:CP-H097
-
miR-4478 Accelerates Nucleus Pulposus Cells Apoptosis Induced by Oxidative Stress by Targeting MTH1(2022/09/20)
作者:Jiafang Zhang, Ruiduan Liu, Ling Mo
期刊:SPINE
DOI:10.1097/BRS.0000000000004486
影响因子 :3.241
引用产品:CP-H097
-
Retinoic acid inhibits the pyroptosis of degenerated nucleus pulposus cells by activating Sirt1-SOD2 signaling(2023/04/02)
作者:Pengfei Li, Fei Xiong, Hongyuan Xing
期刊:CONNECTIVE TISSUE RESEARCH
DOI:10.1080/03008207.2023.2192286
影响因子 :2.900
引用产品:CP-H097
-
Overloaded axial stress activates the Wnt/β-Catenin pathway in nucleus pulposus cells of adult degenerative scoliosis combined with intervertebral disc degeneration(2023/04/08)
作者:Zhijun Cai, Qibiao Luo, Xi Yang
期刊:MOLECULAR BIOLOGY REPORTS
DOI:10.1007/s11033-023-08390-9
影响因子 :2.800
引用产品:CP-H097
-
Role of lncRNA PART1 in intervertebral disc degeneration and associated underlying mechanism(2020/12/10)
作者:Zongyu Zhang, Yongfeng Huo, Zhijing Zhou
期刊:Experimental and Therapeutic Medicine
影响因子 :2.700
引用产品:CP-H097
-
Dimethyl fumarate protects nucleus pulposus cells from inflammation and oxidative stress and delays the intervertebral disc degeneration(2020/10/27)
作者:Hainian Zhu, Gang Chen, Yuhua Wang
期刊:Experimental and Therapeutic Medicine
影响因子 :2.700
引用产品:CP-H097
-
miR‑489‑3p overexpression inhibits lipopolysaccharide‑induced nucleus pulposus cell apoptosis, inflammation and extracellular matrix degradation via targeting Toll‑like receptor 4(2021/9/20)
作者:Ling Dong, Bo Dong
期刊:Experimental and Therapeutic Medicine
影响因子 :2.700
引用产品:CP-H097
-
Experimental study of miR-503 regulating the activity as well as the function of degenerated human nucleus pulposus cells of the intervertebral disc through inhibiting Wnt pathway.(2023/03/01)
作者:Xiaoming Shi, Shaohua Tian, Yinan Tian
期刊:JOURNAL OF MUSCULOSKELETAL & NEURONAL INTERACTIONS
DOI:PMID:36856108
影响因子 :1.900
引用产品:CP-H097
-
Mesenchymal stem cell-derived exosomes are beneficial to suppressing inflammation and promoting autophagy in intervertebral disc degeneration(2023/03/22)
作者:Baicheng Yang, Xinming Yang
期刊:FOLIA MORPHOLOGICA
影响因子 :1.200
引用产品:CP-H097
-
CircRNA RERE Promotes the Oxidative Stress-Induced Apoptosis and Autophagy of Nucleus Pulposus Cells through the miR-299-5p/Galectin-3 Axis(2021/12/15)
作者:Rong Wang, Xingchao Zhou, Guorui Luo
期刊:Journal of Healthcare Engineering
影响因子 :0.000
引用产品:CP-H097
FAQs
Q:{{item.question}}
A:{{item.answer}}
产品资料
识别码示意图