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产品概述
人软骨细胞
Cat NO.: CP-H107
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产品名称:人软骨细胞
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组织来源:软骨组织
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产品规格:5×105cells/T25细胞培养瓶
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细胞简介:
人软骨细胞分离自软骨组织;软骨组织由软骨细胞、基质及纤维构成。软骨组织再生能力强,这些增生的幼稚细胞形似纤维母细胞,以后逐渐变为软骨母细胞,并形成软骨基质,细胞被埋在软骨陷窝内而变为静止的软骨细胞。根据软骨组织内所含纤维成分的不同,可将软骨分为透明软骨、弹性软骨和纤维软骨三种,其中以透明软骨的分布较广,结构也较典型。软骨细胞(chondrocyte)位于软骨陷窝内。幼稚的软骨细胞位于软骨组织的表层,单个分布,体积较小,呈椭圆形,长轴与软骨表面平行,越向深层的软骨细胞体积之间增大呈圆形,细胞核圆形或卵圆形,染色浅,细胞质弱嗜碱性,常见数量不一的脂滴。成熟的软骨细胞多2-8个成群分布于软骨陷窝内,这些软骨细胞由同一个母细胞分裂增殖而成,称为同源细胞群。电镜下,软骨细胞有突起和皱褶,细胞质内有大量的粗面内质网和发达的高尔基复合体及少量的线粒体。在组织切片中,软骨细胞收缩为不规则形,在软骨囊和细胞之间出现较大的腔隙。体外培养的软骨细胞对于研究其生理功能、药物作用以及各种致病因素作用下的病理生理改变具重要意义。
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方法简介:
普诺赛实验室分离的人软骨细胞采用胶原酶 - 中性蛋白酶联合消化制备而来,细胞总量约为5×10⁵cells/瓶。
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质量检测:
普诺赛实验室分离的人软骨细胞经Ⅱ型胶原蛋白免疫荧光鉴定,纯度可达90%以上,且不含有HIV-1、HBV、HCV、支原体、细菌、酵母和真菌等。
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培养信息:
包被条件 培养基 含FBS、生长添加剂、Penicillin、Streptomycin等 产品货号 CM-H107 换液频率 每2-3天换液一次 生长特性 贴壁 细胞形态 梭形、多角形 传代特性 可传5代左右;3代以内状态最佳 消化液 0.25%胰蛋白酶 培养条件 气相:空气,95%;CO2,5% 人软骨细胞体外培养周期有限;建议使用普诺赛配套的专用生长培养基及正确的操作方法来培养,以此保证该细胞的最佳培养状态。
参考文献
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Lubricating Micro-Interface Assisted General Strategy for Preparing dECM-Microparticle–Based Heterogeneous Granular Inks toward 3D Printing(2023/12/11)
作者:Kunxi Zhang, Yinan Zhu, Tao Wang
期刊:ADVANCED FUNCTIONAL MATERIALS
影响因子 :19.000
引用产品:CP-H107
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Fabrication of chondroitin sulfate calcium complex and its chondrocyte proliferation in vitro(2020/10/22)
作者:Qingshan Shen, Chunhui Zhang, Haizhen Mo
期刊:Carbohydrate Polymers
DOI:10.1016/j.carbpol.2020.117282
影响因子 :11.200
引用产品:CP-H107
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HWJMSC-EVs promote cartilage regeneration and repair via the ITGB1/TGF-β/Smad2/3 axis mediated by microfractures(2024/04/12)
作者:Zhian Chen, Tianhua Zhou, Huan Luo
期刊:JOURNAL OF NANOBIOTECHNOLOGY
DOI:10.1186/s12951-024-02451-2
影响因子 :10.200
引用产品:CP-H107
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Plasma exosome miRNA-26b-3p derived from idiopathic short stature impairs longitudinal bone growth via the AKAP2/ERK1/2 axis(2023/03/16)
作者:Xijuan Liu, Jinghong Yuan, Zhiwen Wu
期刊:JOURNAL OF NANOBIOTECHNOLOGY
DOI:10.1186/s12951-023-01849-8
影响因子 :10.200
引用产品:CP-H107
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Circ_SPG11 plays contributing effects on IL-1β-induced chondrocyte apoptosis and ECM degradation via miR-665 inhibition-mediated GREM1 upregulation(2021/11/17)
作者:Xiao Ouyang, Yunzhi Ding, Li Yu
期刊:Clinical Immunology
DOI:10.1016/j.clim.2021.108889
影响因子 :8.600
引用产品:CP-H107
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Silver nanoparticles inhibit the function of hypoxia-inducible factor-1 and target genes: insight into the cytotoxicity and antiangiogenesis(2016/12/08)
作者:Tieshan Yang, Qian Yao, Fei Cao
期刊:International Journal of Nanomedicine
影响因子 :8.000
引用产品:CP-H107
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Activation of microglial GLP-1R in the trigeminal nucleus caudalis suppresses central sensitization of chronic migraine after recurrent nitroglycerin stimulation(2021/07/29)
作者:Feng Jing, Qian Zou, Yangyang Wang
期刊:JOURNAL OF HEADACHE AND PAIN
DOI:10.1186/s10194-021-01302-x
影响因子 :7.400
引用产品:CP-H107
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Circular RNA circANAPC2 mediates the impairment of endochondral ossification by miR‐874‐3p/SMAD3 signalling pathway in idiopathic short stature(2021/03/13)
作者:Xijuan Liu, Zhi Du, Xuan Yi
期刊:Journal Of Cellular And Molecular Medicine
影响因子 :5.300
引用产品:CP-H107
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Hsa_circ_0134111 promotes osteoarthritis progression by regulating miR-224-5p/CCL1 interaction(2021/08/19)
作者:Yongbao Liu, Yanxiu Zhang
期刊:Aging-US
影响因子 :5.200
引用产品:CP-H107
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A Novel Diagnostic Predictive Model for Idiopathic Short Stature in Children(2021/09/07)
作者:Jinghong Yuan, Zhi Du, Zhiwen Wu
期刊:Frontiers in Endocrinology
影响因子 :5.200
引用产品:CP-H107
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Valproic Acid Protects Chondrocytes from LPS-Stimulated Damage via Regulating miR-302d-3p/ITGB4 Axis and Mediating the PI3K-AKT Signaling Pathway(2021/04/22)
作者:Long Sun, Wei Zheng, Qiandong Liu
期刊:Frontiers in Molecular Biosciences
影响因子 :5.000
引用产品:CP-H107
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Hsa_circ_0005567 Activates Autophagy and Suppresses IL-1β-Induced Chondrocyte Apoptosis by Regulating miR-495(2020/08/25)
作者:Jinling Zhang, Fangyue Cheng, Genxiang Rong
期刊:Frontiers in Molecular Biosciences
影响因子 :5.000
引用产品:CP-H107
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Downregulation of miR-3680–3p inhibits the progression of osteoarthritis via targeting OGG1(2022/01/21)
作者:Yanping Xie, Jianwei Li, Yanhui Suo
期刊:Archives of Gerontology and Geriatrics
DOI:10.1016/j.archger.2022.104626
影响因子 :4.000
引用产品:CP-H107
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miR-590-5p affects chondrocyte proliferation, apoptosis, and inflammation by targeting FGF18 in osteoarthritis(2021/08/15)
作者:Peng Jiang, Xixi Dou, Shuaiguang Li
期刊:American Journal of Translational Research
DOI:PMID:34539990
影响因子 :3.940
引用产品:CP-H107
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Regulation of circADAMTS6-miR-324-5p-PIK3R3 ceRNA pathway may be a novel mechanism of IL-1β-induced osteoarthritic chondrocytes(2022/03/25)
作者:Lanjuan Shen, Cheng Ji, Jian Lin
期刊:Journal Of Bone And Mineral Metabolism
DOI:10.1007/s00774-021-01308-0
影响因子 :3.300
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Circ_0136474 promotes the progression of osteoarthritis by sponging mir-140-3p and upregulating MECP2(2022/11/26)
作者:Cheng, Shile, Nie, Zhigang, Cao, Jiarui, Peng, Hao
期刊:JOURNAL OF MOLECULAR HISTOLOGY
DOI:10.1007/s10735-022-10100-x
影响因子 :3.200
引用产品:CP-H107
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Inhibition of miR-182-5p Targets FGF9 to Alleviate Osteoarthritis(2023/03/29)
作者:Yang Sun, Sanmao Su, Mengjun Li
期刊:Analytical Cellular Pathology
影响因子 :3.200
引用产品:CP-H107
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Prognostic-related genes for pancreatic cancer typing and immunotherapy response prediction based on single-cell sequencing data and bulk sequencing data(2023/07/21)
作者:Xuefeng Wang, Sicong Jiang, Xinhong Zhou
期刊:ONCOLOGY RESEARCH
影响因子 :3.100
引用产品:CP-H107
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S100A12 is involved in the pathology of osteoarthritis by promoting M1 macrophage polarization via the NF-κB pathway(2024/03/16)
作者:Yi Zhang, Zihua Li, Cheng Chen
期刊:CONNECTIVE TISSUE RESEARCH
DOI:10.1080/03008207.2024.2310852
影响因子 :2.900
引用产品:CP-H107
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Acrylamide induces human chondrocyte cell death by initiating autophagy‑dependent ferroptosis(2023/04/12)
作者:Hui Wang, Zizheng Tang, Shasha Liu
期刊:Experimental and Therapeutic Medicine
影响因子 :2.700
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Downregulation of lncRNA NEAT1 interacts with miR-374b-5p/PGAP1 axis to aggravate the development of osteoarthritis(2023/09/11)
作者:Feiri Huang, Zhongliang Su, Jie Yang
期刊:Journal of Orthopaedic Surgery and Research
DOI:10.1186/s13018-023-04147-z
影响因子 :2.600
引用产品:CP-H107
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Downregulation of miR-1-3p expression inhibits the hypertrophy and mineralization of chondrocytes in DDH(2021/8/18)
作者:Ding, Rui, Liu, Xijuan, Zhang, Jian
期刊:Journal of Orthopaedic Surgery and Research
DOI:10.1186/s13018-021-02666-1
影响因子 :2.600
引用产品:CP-H107
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Ferroptosis-related genes LPCAT3 and PGD are potential diagnostic biomarkers for osteoarthritis(2023/09/19)
作者:Lufei Wang, Shouxiu Ye, Jianliang Qin
期刊:Journal of Orthopaedic Surgery and Research
DOI:10.1186/s13018-023-04128-2
影响因子 :2.600
引用产品:CP-H107
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Circ_0043947 contributes to interleukin 1β-induced injury in chondrocytes by sponging miR-671-5p to up-regulate RTN3 expression in osteoarthritis pathology(2022/03/24)
作者:Min He, Zhihe Jia, Yiying Wen
期刊:Journal of Orthopaedic Surgery and Research
DOI:10.1186/s13018-022-02970-4
影响因子 :2.600
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Chondrocyte suppression is mediated by miR‐129‐5p via GDF11/SMAD3 signaling in developmental dysplasia of the hip(2020/05/12)
作者:Xijuan Liu, Xueqiang Deng, Rui Ding
期刊:Journal of Orthopaedic Surgery and Research
影响因子 :2.600
引用产品:CP-H107
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Knocking-down long non-coding RNA LINC01094 prohibits chondrocyte apoptosis via regulating microRNA-577/metal-regulatory transcription factor 1 axis(2024/05/17)
作者:Feiri Huang, Zhongliang Su, Jie Yang
期刊:Journal of Orthopaedic Surgery
影响因子 :1.600
引用产品:CP-H107
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