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产品概述
| 名称 | PLC/PRF/5 (人肝癌亚力山大细胞) (STR鉴定正确) |
| 别称 | PLC-PRF-5; PLC PRF 5; PLC/PRF5; PLCPRF5; PLC-8024; PLC8024; PLC; Alexander cells; Alexander; Primary Liver Carcinoma/Poliomyelitis Research Foundation/5 |
| 种属 | 人 |
| 生长特性 | 贴壁细胞 |
| 细胞形态 | 上皮细胞样 |
| 冻存条件 | 冻存液：55% 基础培养基+40%FBS+5%DMSO 温度：液氮 |
| 培养方案A（默认） |
生长培养基:
培养条件:
气相：空气，95%；CO2，5%, 温度：37℃
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| 推荐传代比例 | 1:2-1:4 |
| 推荐换液频率 | 2-3次/周 |
| 背景描述 | PLC/PRF/5细胞分泌乙肝病毒表面抗原(HBsAg)；PLC/PRF/5细胞原先被支原体污染，后用BM-cycline去除支原体。 |
| 组织来源 | 肝癌；亚力山大细胞 |
| 细胞类型 | 肿瘤细胞 |
| 肿瘤类型 | 肝胆癌细胞 |
| 生物安全等级 | BSL-2 [Cells contain hepatitis B] |
| 倍增时间 | ~36-48 hours |
| 基因表达情况 | hepatitis virus B surface antigen (HBsAg) |
| 保藏机构 | ATCC; CRL-8024 ECACC; 85061113 |
STR鉴定
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STR位点信息
Amelogenin X CSF1PO 10 D2S1338 19,22 D3S1358 15 D5S818 12 D7S820 9,11 D8S1179 13,16 D13S317 11,12 D16S539 13 D18S51 17 D19S433 11,13 D21S11 30,33.2 FGA 19.2,25 PentaD 6,10 PentaE 10,16 TH01 8 TPOX 8 vWA 15,16 D6S1043 13,21 D12S391 15,20 D2S441 12,14 -
STR鉴定图
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参考文献
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Targeting DDX11 promotes PARP inhibitor sensitivity in hepatocellular carcinoma by attenuating BRCA2-RAD51 mediated homologous recombination(2023/11/25)
作者：Kun Cao, Ruonan Wang, Lianhai Li
期刊：ONCOGENE
DOI：10.1038/s41388-023-02898-x
影响因子 ：8.000
引用产品：CL-0415
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RBBP7, regulated by SP1, enhances the Warburg effect to facilitate the proliferation of hepatocellular carcinoma cells via PI3K/AKT signaling(2024/02/18)
作者：Yuan Fang, WeiQiang Tang, Siming Qu
期刊：Journal of Translational Medicine
DOI：10.1186/s12967-024-04964-8
影响因子 ：7.400
引用产品：CL-0415
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VEZF1, destabilized by STUB1, affects cellular growth and metastasis of hepatocellular carcinoma by transcriptionally regulating PAQR4(2022/10/14)
作者：Xiaobao Shi, Pengfei Zhao, Gang Zhao
期刊：Cancer Gene Therapy
DOI：10.1038/s41417-022-00540-8
影响因子 ：6.400
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SLC39A10 Upregulation Predicts Poor Prognosis, Promotes Proliferation and Migration, and Correlates with Immune Infiltration in Hepatocellular Carcinoma(2022/11/14)
作者：Zuyi Ma, Zhenchong Li, Shujie Wang
期刊：Journal of Hepatocellular Carcinoma
影响因子 ：4.962
引用产品：CL-0415
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PAQR4 promotes the development of hepatocellular carcinoma by activating PI3K/AKT pathway(2021/10/30)
作者：Gang Zhao, Xiaobao Shi, Zhanbo Sun
期刊：Acta Biochimica Et Biophysica Sinica
影响因子 ：3.511
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Combining a glycolysis‑related prognostic model based on scRNA‑Seq with experimental verification identifies ZFP41 as a potential prognostic biomarker for HCC(2024/03/19)
作者：Yu Teng, Jianrong Xu, Yaoqun Wang
期刊：Molecular Medicine Reports
影响因子 ：3.400
引用产品：CL-0415
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Silencing of keratin 15 impairs viability and mobility while facilitating the doxorubicin chemosensitivity by inactivating the β‑catenin pathway in liver cancer(2023/08/30)
作者：Junying Wang, Guangyu Zhu
期刊：Oncology Letters
影响因子 ：2.900
引用产品：CL-0102 CL-0103 CL-0120 CL-0139 CL-0415 PB180120 PM150110 PM150210
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The diagnostic, prognostic, drug sensitivity and ceRNA internet of PPP4R1 in liver hepatocellular carcinoma (LIHC)(2024/02/26)
作者：Xiaowei Li, Yinglian Pan, Siren Feng
期刊：Translational Cancer Research
影响因子 ：0.900
引用产品：CL-0415
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