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产品概述
名称 | MDCK [NBL-2] (犬肾细胞) (种属鉴定正确) |
别称 | MDCK (NBL-2); NBL-2; Madin-Darby Canine Kidney; Madin Darby Canine Kidney |
种属 | 狗 |
生长特性 | 贴壁细胞 |
细胞形态 | 上皮细胞样 |
冻存条件 | 冻存液:55% 基础培养基+40%FBS+5%DMSO 温度:液氮 |
培养方案A(默认) |
生长培养基:
培养条件:
气相:空气,95%;CO2,5%, 温度:37℃
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推荐传代比例 | 1:2-1:4 |
推荐换液频率 | 2-3次/周 |
背景描述 | MDCK(NBL-2)细胞是由S·H·Madin和N·B·Darby在1958年9月从一只外观正常的成年雌性英国小猎犬的肾分离得到的;MDCK(NBL-2)细胞角蛋白免疫过氧化物酶染色呈阳性;MDCK(NBL-2)细胞被用于研究β-粥样蛋白前体的处理及其蛋白水解产物。 |
年龄(性别) | 雌性;成年 |
组织来源 | 肾 |
细胞类型 | 自发永生化细胞 |
生物安全等级 | BSL-1 |
基因表达情况 | The cells are positive for keratin by immunoperoxidase staining. |
保藏机构 | ATCC; CCL-34 ATCC; CRL-6253ECACC; 84121903 ECACC; 85011435 |
种属鉴定
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种属鉴定图
参考文献
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Exploration of the protective mechanisms of Icariin against cisplatin-induced renal cell damage in canines (2024-02-21)
作者:Jiayi Liu:1.College of Police Dog Technology of Criminal Investigation Police University of China, Shenyang, China. ; Liuwei Xie:1.College of Police Dog Technology of Criminal Investigation Police University of China, Shenyang, China. ; He Zhai:1.College of Police Dog Technology of Criminal Investigation Police University of China, Shenyang, China. ; Dongwei Wang:1.College of Police Dog Technology of Criminal Investigation Police University of China, Shenyang, China. ; Xiao Li:1.Institute of Special Wild Economic Animals and Plants, Chinese Academy of Agricultural Sciences, Changchun, China. ; Yao Wang:1.College of Police Dog Technology of Criminal Investigation Police University of China, Shenyang, China. ; Mingqiang Song:1.College of Police Dog Technology of Criminal Investigation Police University of China, Shenyang, China. ; Chao Xu:1.Institute of Special Wild Economic Animals and Plants, Chinese Academy of Agricultural Sciences, Changchun, China. ;
期刊:Exploration of the protective mechanisms of Icariin against cisplatin-induced renal cell damage in canines
DOI:10.3389/fvets.2024.1331409
影响因子 :3.2
引用产品: MDBK [NBL-1] 细胞
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Endoplasmic reticulum stress contributes to pyroptosis through NF-κB/NLRP3 pathway in diabetic nephropathy (2023-04-01)
作者:Quanwei Li:1.College of Veterinary Medicine, South China Agricultural University, Guangzhou 510642, Guangdong, PR China. ; Kai Zhang:1.College of Veterinary Medicine, South China Agricultural University, Guangzhou 510642, Guangdong, PR China. ; Limin Hou:1.College of Veterinary Medicine, South China Agricultural University, Guangzhou 510642, Guangdong, PR China. ; Jianzhao Liao:1.College of Veterinary Medicine, South China Agricultural University, Guangzhou 510642, Guangdong, PR China. ; Hui Zhang:1.College of Veterinary Medicine, South China Agricultural University, Guangzhou 510642, Guangdong, PR China. ; Qingyue Han:1.College of Veterinary Medicine, South China Agricultural University, Guangzhou 510642, Guangdong, PR China. ; Jianying Guo:1.College of Veterinary Medicine, South China Agricultural University, Guangzhou 510642, Guangdong, PR China. ; Ying Li:1.College of Veterinary Medicine, South China Agricultural University, Guangzhou 510642, Guangdong, PR China. ; Lianmei
期刊:Endoplasmic reticulum stress contributes to pyroptosis through NF-κB/NLRP3 pathway in diabetic nephropathy
影响因子 :6.1
引用产品: 特级胎牛血清 , MDBK [NBL-1] 细胞
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Polydopamine-coated mesoporous silica nanoparticles co-loaded with Ziyuglycoside I and Oseltamivir for synergistic treatment of viral pneumonia (2023-09-12)
作者:Hong Wang:1.Key Laboratory of Basic Pharmacology of Guizhou Province and School of Pharmacy, Zunyi Medical University, Zunyi, Guizhou 563000, China; Key Laboratory of Basic Pharmacology of Ministry of Education and Joint International Research Laboratory of Ethnomedicine of Ministry of Education, Zunyi Medical University, Zunyi, Guizhou 563000, China. ; Lei Chen:1.Key Laboratory of Basic Pharmacology of Guizhou Province and School of Pharmacy, Zunyi Medical University, Zunyi, Guizhou 563000, China; Key Laboratory of Basic Pharmacology of Ministry of Education and Joint International Research Laboratory of Ethnomedicine of Ministry of Education, Zunyi Medical University, Zunyi, Guizhou 563000, China. ; Ruidong Li:1.Key Laboratory of Basic Pharmacology of Guizhou Province and School of Pharmacy, Zunyi Medical University, Zunyi, Guizhou 563000, China; Key Laboratory of Basic Pharmacology of Ministry of Education and Joint International Research Laboratory of Ethnomedicine of Ministry of
期刊:Polydopamine-coated mesoporous silica nanoparticles co-loaded with Ziyuglycoside I and Oseltamivir for synergistic treatment of viral pneumonia
DOI:10.1016/j.ijpharm.2023.123412
影响因子 :5.8
引用产品: 增强型细胞活力检测试剂盒 (CCK-8) , MDBK [NBL-1] 细胞 , A549 [A-549] 细胞 , RAW 264.7 细胞
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Enhancing both oral bioavailability and anti-ischemic stroke efficacy of ginkgolide B by preparing nanocrystals self-stabilized Pickering nano-emulsion (2023-10-21)
作者:Yun Liu: 1.School of Pharmacy, Liaoning University of Traditional Chinese Medicine, No. 77, Life One Road DD port, Dalian 116600, China; Chungang Zhang: 1.School of Pharmacy, Liaoning University of Traditional Chinese Medicine, No. 77, Life One Road DD port, Dalian 116600, China;2.Department of Pharmacy, Changzhi Medical College, Changzhi, China;3.Key Laboratory of Ministry of Education, Traditional Chinese Medicine Viscera-State Theory and Applications, Liaoning University of Traditional Chinese Medicine, Shenyang, China;4.Qimeng Co., LTD, Chifeng, China; Lan Cheng: 1.School of Pharmacy, Liaoning University of Traditional Chinese Medicine, No. 77, Life One Road DD port, Dalian 116600, China; Hongxin Wang: 1.Key Laboratory of Cardiovascular and Cerebrovascular Drug Research of Liaoning Province, Jinzhou Medical University, Jinzhou, Liaoning Province, China; Meili Lu: 1.Key Laboratory of Cardiovascular and Cerebrovascular Drug Research of Liaoning Province, Jinzhou Medical University, J
期刊:Enhancing both oral bioavailability and anti-ischemic stroke efficacy of ginkgolide B by preparing nanocrystals self-stabilized Pickering nano-emulsion
DOI:10.1016/j.ejps.2023.106620
影响因子 :4.6
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Protective effect of chrysophanol on canine renal cell injury induced by canine parvovirus (2023-05-31)
作者:Ting Gong:1.Henan University of Animal Husbandry and Economy, Zhengzhou, 450000, China. gongting8301@126.com. ;
期刊:Protective effect of chrysophanol on canine renal cell injury induced by canine parvovirus
影响因子 :1.6
引用产品: MDBK [NBL-1] 细胞
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STIM1 mediates IAV-induced inflammation of lung epithelialcells by regulating NLRP3 and inflammasome activation viatargeting miR-223 (2012-00-20)
作者:Cui-Cui Liu:1.Department of Respiratory and Asthma, Xi'an Children's Hospital, Xi'an 710003, China. ; Yi Miao:1.Department of Respiratory and Critical Care Medicine, Shaanxi Provincial People's Hospital, Xi'an 710068, Shaanxi, China. ; Rui-Lin Chen:1.Department of Respiratory and Critical Care Medicine, Shaanxi Provincial People's Hospital, Xi'an 710068, Shaanxi, China. ; Yong-Qing Zhang:1.Department of Respiratory and Critical Care Medicine, Shaanxi Provincial People's Hospital, Xi'an 710068, Shaanxi, China. ; Hua Wu:1.Department of Respiratory and Critical Care Medicine, Shaanxi Provincial People's Hospital, Xi'an 710068, Shaanxi, China. ; Shu-Mei Yang:1.Department of Respiratory and Critical Care Medicine, Shaanxi Provincial People's Hospital, Xi'an 710068, Shaanxi, China. ; Li-Qun Shang:1.Department of Respiratory and Critical Care Medicine, Shaanxi Provincial People's Hospital, Xi'an 710068, Shaanxi, China. Electronic address: liqun333@foxmail.com. ;
期刊:STIM1 mediates IAV-induced inflammation of lung epithelialcells by regulating NLRP3 and inflammasome activation viatargeting miR-223
影响因子 :6.1
引用产品: BEAS-2B , MDBK [NBL-1] 细胞
FAQs
Q:为什么我看的文献里的细胞培养条件和你们官网的培养条件不一样呢?
A:部分细胞是会出现多种培养条件的,我们公司优先选择引种来源的培养条件以及建系者所用培养条件,出现差异的原因是不同实验室在保藏过程中更改了细胞的培养条件,为了避免细胞突然更换培养条件后不适应,建议您优先使用厂家推荐的培养条件培养。
Q:冻存细胞运输与常温细胞运输相比有什么区别?
A:发货有两种形式,常温或者冻存都可以的,常温就是货期一周左右(具体以购买当时销售报的货期为准),我们培养好一个T25瓶发货;冻存细胞如果有库存下单第二天能发货,细胞系冻存细胞担心客户复苏不成功所以赠送了一管,实际发货2管;冻存细胞发货是10公斤干冰及顺丰运输,所以冻存细胞需额外支付干冰及运输费。
Q:培养细胞的培养瓶可以重复利用吗?
A:一般不建议重复利用,特别是贴壁不牢固细胞,重复利用会导致吸附性变差,漂浮增多;一个T25瓶建议使用最多不超过3次,其次需要注意无菌操作,避免污染。
Q:冻存的细胞出现颜色不一致,有的红有的粉,对复苏会有影响吗?
A:这种情况容易在不同冻存批次间出现,与冻存细胞的密度、冻存液配方、温度导致pH变化等有关,偶尔有遇到这种情况,不是绝对性对细胞状态有影响,可以复苏看下。
Q:不同引种单位的同一株细胞,RRID都是一样的吗?
A:是的,同一个细胞系只有一个RRID。
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