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产品概述
名称 | Beta-TC-6 (小鼠胰岛素瘤胰岛β细胞) (STR鉴定正确) |
别称 | beta-TC6; beta-TC-6; BetaTC6; betaTC6 |
种属 | 小鼠 |
生长特性 | 贴壁细胞 |
细胞形态 | 上皮细胞样 |
冻存条件 | 冻存液:55% 基础培养基+40%FBS+5%DMSO 温度:液氮 |
培养方案A(默认) |
培养条件:
气相:空气,95%;CO2,5%, 温度:37℃
|
推荐传代比例 | 1:2-1:4 |
推荐换液频率 | 2-3次/周 |
背景描述 | Beta-TC-6细胞来源于转基因小鼠中生长的一个胰肿瘤(胰岛素瘤),这种小鼠携带了大鼠胰岛素Ⅱ基因启动子调控的SV40早期基因的假基因结构。Beta-TC-6细胞包含大量的胰岛素和小量的胰高血糖素及生长抑素;Beta-TC-6细胞能响应葡萄糖而分泌胰岛素。 |
年龄(性别) | 不详 |
组织来源 | 胰;胰岛素瘤 |
细胞类型 | 肿瘤细胞 |
肿瘤类型 | 胰腺癌细胞 |
生物安全等级 | BSL-2 [Cells contain SV40 viral DNA Sequences] |
基因表达情况 | insulin, glucagon, and somatostatin |
保藏机构 | ATCC; CRL-11506 |
STR鉴定
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STR位点信息
18-3 16 6-7 12,17 5-5 17 X-1 28 1-2 17,19 7-1 26.2,27 8-1 16 1-1 17 19-2 12,13 15-3 21.3,22.3 12-1 17 6-4 18 4-2 20.3 3-2 14 2-1 16 13-1 17.1
11-2 16,17 17-2 15 -
STR鉴定图
参考文献
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Evaluating the In Situ Insulinotropic Effects of Pea Protein Hydrolysates Mediated by Active GLP-1 via a 2D and Dual-Layered Coculture Cell Model(2023/09/18)
作者:Mingkai Zhang, Ling Zhu, Hui Zhang
期刊:JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
影响因子 :6.100
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Transepithelial Transport of the Bifunctional Peptide IPYWTY Indirectly Induced Insulin Release Mediated by Active GLP-1(2023/08/17)
作者:Mingkai Zhang, Ling Zhu, Hui Zhang
期刊:JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
影响因子 :6.100
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Cdc42 upregulation under high glucose induces podocyte apoptosis and impairs β-cell insulin secretion(2022/08/10)
作者:Shan Jiang, Chunmei Xu, Shuai Yao
期刊:Frontiers in Endocrinology
影响因子 :6.055
引用产品:CL-0322
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RGS7 silence protects palmitic acid-induced pancreatic β-cell injury by inactivating the chemokine signaling pathway(2023/03/31)
作者:Yurong Zhu, Jun Li, Tao Ba
期刊:AUTOIMMUNITY
DOI:10.1080/08916934.2023.2194584
影响因子 :3.500
引用产品:CL-0322
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A peptide encoded by lncRNA MIR7-3 host gene (MIR7-3HG) alleviates dexamethasone-induced dysfunction in pancreatic β-cells through the PI3K/AKT signaling pathway(2023/01/03)
作者:Xiaoming Mao, Jinliang Zhou, Zhiyu Zhang
期刊:BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
DOI:10.1016/j.bbrc.2023.01.004
影响因子 :3.100
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Silencing of DIRAS3 improves the proliferation and insulin secretion of palmitic acid-treated pancreatic β‑cells through regulating PI3K/AKT signaling(2023/09/04)
作者:Ying Li, Shan Gao, Ying Yang
期刊:International Journal of Diabetes in Developing Countries
DOI:10.1007/s13410-023-01240-1
影响因子 :0.900
引用产品:CL-0322
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