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产品概述
名称 | DC2.4 (小鼠骨髓来源树突状细胞) (STR鉴定正确) |
别称 | DC 2.4 |
种属 | 小鼠 |
生长特性 | 贴壁细胞,少量悬浮 |
细胞形态 | 圆形;梭形,胞突呈分枝状 |
冻存条件 | 冻存液:55% 基础培养基+40%FBS+5%DMSO 温度:液氮 |
培养方案A(默认) |
培养条件:
气相:空气,95%;CO2,5%, 温度:37℃
|
推荐传代比例 | 1:2-1:4 |
推荐换液频率 | 2-3天/次 |
注意事项 | 圆形贴壁,少量贴壁细胞伸展开,少量悬浮。密度低时漂浮较少,密度高时,漂浮比例增大,漂浮部分也可以培养。 |
背景描述 | DC2.4是通过用表达小鼠粒细胞-巨噬细胞-CSF(GM-CSF)和myc和raf致癌基因的逆转录病毒载体转导C57BL/6小鼠的骨髓分离物而产生的永生化小鼠树突状细胞。DC2.4表现出树突细胞的特征,包括细胞形态和树突细胞特异性标记物的表达,以及吞噬MHC I类和II类分子并在其上呈递外源性抗原的能力。 |
细胞类型 | 转化细胞系 |
生物安全等级 | BSL-1 |
STR鉴定
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STR位点信息
18-3 15,16 6-7 17 5-5 17,18 X-1 28 1-2 19 7-1 26.2,27.2 8-1 16 1-1 17 19-2 13 15-3 22.3,23.3 12-1 17 6-4 18 4-2 20.3,21.3 3-2 14 2-1 16 13-1 17.1
11-2 16,17 17-2 16 -
STR鉴定图
参考文献
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Nanoadjuvant-triggered STING activation evokes systemic immunotherapy for repetitive implant-related infections(2024/01/24)
作者:Dongdong Xu, Jun Hu, Jiawei Mei
期刊:Bioactive Materials
DOI:10.1016/j.bioactmat.2024.01.020
影响因子 :18.900
引用产品:CL-0545
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Tailoring therapeutics via a systematic beneficial elements comparison between photosynthetic bacteria-derived OMVs and extruded nanovesicles(2024/02/26)
作者:Tingshan Xiao, Yichuan Ma, Ziyang Zhang
期刊:Bioactive Materials
DOI:10.1016/j.bioactmat.2024.02.025
影响因子 :18.900
引用产品:CL-0545
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Cancer cell membrane proteins-encapsulated nanovaccine enhances cancer immunotherapy and prevention by provoking antigen-specific cellular immunity via the dendritic cell-targeted delivery(2024/01/06)
作者:Xingyu Luo, Xiaolu Chen, Rongying Ma
期刊:CHEMICAL ENGINEERING JOURNAL
影响因子 :15.100
引用产品:CL-0545
-
Pre-Induced ICD Membrane-Coated Carrier-Free Nanoparticles for the Personalized Lung Cancer Immunotherapy(2023/03/18)
作者:Shilin Li, Shasha Jiang, Muhammad Saif Ur Rahman
期刊:Small Methods
影响因子 :12.400
引用产品:CL-0545
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Delivery of mRNA Vaccine with 1, 2-Diesters-Derived Lipids Elicits Fast Liver Clearance for Safe and Effective Cancer Immunotherapy(2023/11/21)
作者:Yuandong Xu, Yuexiao Hu, Heng Xia
期刊:Advanced Healthcare Materials
影响因子 :10.000
引用产品:CL-0545
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PTT/ PDT-induced Microbial Apoptosis and Wound Healing Depend on Immune Activation and Macrophage Phenotype Transformation(2023/06/25)
作者:Haoyu Chen, Lijuan Wu, Tianyi Wang
期刊:Acta Biomaterialia
DOI:10.1016/j.actbio.2023.06.025
影响因子 :9.700
-
Extracellular vesicles hybrid plasmid-loaded lipid nanovesicles for synergistic cancer immunotherapy(2023/10/27)
作者:Qing Tong, Kexin Li, Fanwei Huang
期刊:Materials Today Bio
DOI:10.1016/j.mtbio.2023.100845
影响因子 :8.200
引用产品:CL-0545
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Na2S2O4@Co-metal organic framework (ZIF-67) @glucose oxidase for biofilm-infecting wound healing with immune activation(2022/11/30)
作者:Guangye Ge, Lijuan Wu, Fenglan Zhang
期刊:INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
DOI:10.1016/j.ijbiomac.2022.11.265
影响因子 :8.025
引用产品:CL-0545
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Doxorubicin-Loaded Platelet Decoys for Enhanced Chemoimmunotherapy Against Triple-Negative Breast Cancer in Mice Model(2023/06/30)
作者:Hang Dong, Meng Gao, Lu Lu
期刊:International Journal of Nanomedicine
影响因子 :8.000
-
Cascade catalysis-coordinated nanorobot toward synergistic cancer chemoimmunotherapy(2023/08/30)
作者:Ruirui Guo, Bin Wang, Dong Liu
期刊:Journal of Materials Chemistry B
影响因子 :7.000
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Interferon-γ regulates immunosuppression in septic mice by promoting the Warburg effect through the PI3K/AKT/mTOR pathway(2023/07/11)
作者:Xuzhe Fu, Yu Wang
期刊:MOLECULAR MEDICINE
DOI:10.1186/s10020-023-00690-x
影响因子 :5.700
-
Dendritic cells originating exosomal miR-193b-3p induces regulatory T cells to alleviate liver transplant rejection(2022/12/21)
作者:Bin Cui, XiaoJie Chen, Jie Sun
期刊:INTERNATIONAL IMMUNOPHARMACOLOGY
DOI:10.1016/j.intimp.2022.109541
影响因子 :5.600
引用产品:CL-0545
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Transcriptomic analysis reveal the responses of dendritic cells to VDBP(2022/03/12)
作者:Biwei Cao, Tao Wen, Meng Wei
期刊:Genes & Genomics
DOI:10.1007/s13258-022-01234-z
影响因子 :2.100
引用产品:CL-0545
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