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产品概述
平衡盐溶液(Balanced Salt Solution, BSS)也叫生理溶液(Physiological Solution),是集缓冲液的缓冲力、生理盐水的等渗性以及培养液的营养供应特点于一体的,具有维持渗透压、保持PH稳定以及提供简单的营养的作用,可满足体外实验中组织、器官或细胞的生存和代谢的基本需要。一些平衡盐溶液中还添加了少量酚红,用以指示溶液的酸碱度变化。
Hank's平衡盐溶液(Hank's Balanced Salt Solution, HBSS)是细胞分离或培养中常用的磷酸盐缓冲液之一,主要成分包括NaCl、KCl、KH2PO4、Na2HPO4、NaHCO3、CaCl2、MgCl2、MgSO4和葡萄糖。Hank's平衡盐溶液常用于组织块的漂洗、细胞的洗涤、细胞或组织的运输、其他试剂的配制以及作为细胞计数的稀释液等。
产品信息
形态 | 液体 |
浓度 | 1× |
规格 | 500mL |
pH | 7.0-7.4 |
CaCl2(无水) | 140mg/L |
MgCl2•6H2O | 100mg/L |
MgSO4•7H2O | 100mg/L |
NaHCO3 | 350mg/L |
D-葡萄糖 | 1000mg/L |
酚红指示剂 | 无 |
储存条件 | 2-30℃ |
运输条件 | 室温 |
有效期 | 36个月 |
注意事项
1、本产品为即用型,经0.1μm过滤除菌,可直接使用,使用前无需稀释或过滤除菌等任何处理;
2、使用本产品时应注意无菌操作,避免污染;
3、由于Hank's平衡盐溶液含有钙镁离子,易产生少量沉淀,一般温浴后沉淀即可溶解;若沉淀较多且又无法溶解时,请放弃使用;
4、由于Hank's平衡盐溶液NaHCO3含量较低,不能用于5% CO2的环境,若放入CO2培养箱,溶液将迅速变酸,使用时应注意;
5、本产品仅用于科研或进一步研究使用,不用于诊断和治疗。
参考文献
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作者:Jingzhe Niu,Yanhua Guo, Kai Li
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DOI:10.1016/j.msec.2021.111917
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作者:Jun Deng, Hui Ke
期刊:OncoImmunology
DOI:10.1080/2162402X.2022.2152635
影响因子 :7.200
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作者:Guanfei Zhang, Min Wang, Yilin Gao
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Preparation and characterization of a novel composite acellular matrix/hyaluronic acid thermosensitive hydrogel for interstitial cystitis/bladder pain syndrome(2023/11/16)
作者:Wei Guo, Haichao Liu, Jiaxing Zhang
期刊:JOURNAL OF BIOMEDICAL MATERIALS RESEARCH PART A
影响因子 :4.900
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Electrical stimulation activates calpain 2 and subsequently upregulates collagens via the integrin β1/TGF-β1 signaling pathway(2019/3/30)
作者:Yang Li, Cheng Liu, Bingshu Li
期刊:Cellular Signalling
DOI:10.1016/j.cellsig.2019.03.023
影响因子 :4.800
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作者:XiaoLei Zhang, Yu Wang, Jia Lv
期刊:NEUROCHEMISTRY INTERNATIONAL
DOI:10.1016/j.neuint.2024.105683
影响因子 :4.200
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Pharmacological effects of Pugionium cornutum (L.) Gaertn. extracts on gastrointestinal motility are partially mediated by quercetin(2021/09/03)
作者:Chencan Su, Haoyu Li, Bang Chen
期刊:BMC Complementary Medicine and Therapies
DOI:10.1186/s12906-021-03395-y
影响因子 :3.900
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作者:Xinghui Li, Qi Liang, Lu Liu
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DOI:10.1080/0886022X.2024.2322037
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