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产品概述
名称 | SiHa (人子宫颈鳞癌细胞) (STR鉴定正确) |
别称 | Siha; SIHA |
种属 | 人 |
生长特性 | 贴壁细胞 |
细胞形态 | 上皮细胞样 |
冻存条件 | 冻存液:55% 基础培养基+40%FBS+5%DMSO 温度:液氮 |
培养方案A(默认) |
生长培养基:
培养条件:
气相:空气,95%;CO2,5%, 温度:37℃
|
推荐传代比例 | 1:3-1:4 |
推荐换液频率 | 2-3次/周 |
背景描述 | SiHa细胞是建自一个日本病人的外科手术的原位组织样品。电镜观察表明,在SiHa细胞连接处有典型的桥粒,在SiHa细胞胞质中有丰富的张力丝。在裸鼠中,SiHa细胞能形成低分化的表皮样癌(Ⅲ级);SiHa细胞中,癌基因PRB和p53阳性。 |
年龄(性别) | 女性;55岁 |
组织来源 | 子宫颈鳞癌 |
细胞类型 | 肿瘤细胞 |
肿瘤类型 | 宫颈癌细胞 |
生物安全等级 | BSL-2 [Cells contain human papilloma virus] |
倍增时间 | ~50-60 hours |
致瘤性 | Yes, in nude mice; forms poorly differentiated epidermoid carcinoma (grade III). |
基因表达情况 | Oncogenes: p53+; pRB+ |
保藏机构 | ATCC; HTB-35 |
STR鉴定
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STR位点信息
Amelogenin X CSF1PO 12 D2S1338 24 D3S1358 16,17 D5S818 9 D7S820 10 D8S1179 13,16 D13S317 11 D16S539 12 D18S51 15 D19S433 14.2 D21S11 31 FGA 21 PentaD 9 PentaE 10,12 TH01 6,9 TPOX 8 vWA 14,17 D6S1043 18 D12S391 19,22 D2S441 10 -
STR鉴定图
参考文献
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Gene signatures, immune infiltration, and drug sensitivity based on a comprehensive analysis of m6a RNA methylation regulators in cervical cancer(2022/09/04)
作者:Xiaoqin Lu, Rui Li, Yanqi Ying
期刊:Journal of Translational Medicine
DOI:10.1186/s12967-022-03600-7
影响因子 :7.400
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Design of Selective PARP-1 Inhibitors and Antitumor Studies(2024/05/22)
作者:Yiting Zhang, Xiangqian Li, Fang Liu
期刊:JOURNAL OF MEDICINAL CHEMISTRY
DOI:10.1021/acs.jmedchem.3c02460
影响因子 :7.300
引用产品:CL-0210
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Acetylation-stabilized chloride intracellular channel 1 exerts a tumor-promoting effect on cervical cancer cells by activating NF-κB(2021/01/19)
作者:Wanyue Wang, Xin Li, Ye Xu
期刊:Cellular Oncology
DOI:10.1007/s13402-020-00582-w
影响因子 :6.600
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Prognosis, immune microenvironment, and personalized treatment prediction in Rho GTPase-activating protein 4-mutant cervical cancer: Computer strategies for precision oncology(2023/01/03)
作者:Xiaoqin Lu, Yanqi Ying, Wenyi Zhang
期刊:Life Sciences
影响因子 :6.100
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Long Intervening Noncoding 00467 RNA Contributes to Tumorigenesis by Acting as a Competing Endogenous RNA against miR-107 in Cervical Cancer Cells(2019/11/01)
作者:Guangcai Li, Li Xin, Yongsheng Wang
期刊:Am J Pathol
DOI:10.1016/j.ajpath.2019.07.012
影响因子 :6.000
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Overexpression of mircoRNA-137 inhibits cervical cancer cell invasion, migration and epithelial–mesenchymal transition by suppressing the TGF-β/smad pathway via binding to GREM1(2019/05/23)
作者:Hui Miao, Nuan Wang, Linxin Shi
期刊:Cancer Cell International
影响因子 :5.800
引用产品:CL-0210
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Synergistic effects of sodium butyrate and cisplatin against cervical carcinoma in vitro and in vivo(2022/10/21)
作者:Huijun Chu, Xiaoyuan Sun, Jia Wang
期刊:Frontiers in Oncology
影响因子 :5.738
引用产品:CL-0210
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Methionine enkephalin inhibited cervical carcinoma via apoptosis promotion and reduction of myeloid derived suppressor cell infiltrated in tumor(2022/06/20)
作者:Na Qu, Ruizhe Wang, Yiming Meng
期刊:INTERNATIONAL IMMUNOPHARMACOLOGY
DOI:10.1016/j.intimp.2022.108933
影响因子 :5.714
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3-Hydroxymorindone from Knoxia roxburghii (Spreng.) M. A. Rau induces ROS-mediated mitochondrial dysfunction cervical cancer cells apoptosis via inhibition of PI3K/AKT/NF-κB signaling pathway(2023/03/11)
作者:Xiaojiao Chen, Zhibo Liu, Xue Li
期刊:Journal of Functional Foods
影响因子 :5.600
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Silencing DTX3L Inhibits the Progression of Cervical Carcinoma by Regulating PI3K/AKT/mTOR Signaling Pathway(2023/01/03)
作者:Wei Hu, Yaorui Hu, Yao Pei
期刊:INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
影响因子 :5.600
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Methionine enkephalin inhibited cervical cancer migration as well as invasion and activated CD11b+ NCR1+ NKs of tumor microenvironment(2023/09/21)
作者:Na Qu, Yiming Meng, Jingbo Zhai
期刊:INTERNATIONAL IMMUNOPHARMACOLOGY
DOI:10.1016/j.intimp.2023.110967
影响因子 :5.600
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The anticancer effect of extract of medicinal mushroom Sanghuangprous vaninii against human cervical cancer cell via endoplasmic reticulum stress-mitochondrial apoptotic pathway(2021/6/17)
作者:Pingya He, Yuhao Hou, Yue Yang
期刊:Journal Of Ethnopharmacology
影响因子 :5.400
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USF1 regulated circPRDM4 modulates tumorigenesis and immune escape in chemoresistant cervical cancer(2023/09/04)
作者:Yan Zhang, Xing Li, Jun Zhang
期刊:JOURNAL OF CELLULAR AND MOLECULAR MEDICINE
影响因子 :5.300
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KMT2A regulates cervical cancer cell growth through targeting VDAC1.(2020/05/21)
作者:Changlin Zhang, Yijun Hua, Huijuan Qiu
期刊:Aging-US
影响因子 :5.200
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CDK6 increases glycolysis and suppresses autophagy by mTORC1-HK2 pathway activation in cervical cancer cells(2022/02/15)
作者:Xiaoxi Zhang, Yunxia Sun, Siyao Cheng
期刊:Cell Cycle
DOI:10.1080/15384101.2022.2039981
影响因子 :5.173
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Integral membrane protein 2A enhances sensitivity to chemotherapy via notch signaling pathway in cervical cancer(2021/12/07)
作者:Yan Li, Jianhua Wang, Chengzhen Gao
期刊:Bioengineered
DOI:10.1080/21655979.2021.2001218
影响因子 :4.900
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Kinetochore-associated protein 1 promotes the invasion and tumorigenicity of cervical cancer cells via matrix metalloproteinase-2 and matrix metalloproteinase-9(2022/04/07)
作者:Caimei Wang, Yiyuan Wang, Congrong Liu
期刊:Bioengineered
DOI:10.1080/21655979.2022.2061144
影响因子 :4.900
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Lymph node metastasis-related gene signature shows good performance in predicting prognosis and immune infiltration in cervical cancer(2023/06/22)
作者:Yilin Guo, Lu Wang, Zhen Xu
期刊:Frontiers in Oncology
影响因子 :4.700
引用产品:CL-0210
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The CXCLs-CXCR2 axis modulates the cross-communication between tumor-associated neutrophils and tumor cells in cervical cancer(2024/01/15)
作者:Haizhou Ji, Bin Liu, Mi Ren
期刊:Expert Review of Clinical Immunology
DOI:10.1080/1744666X.2024.2305808
影响因子 :4.400
引用产品:CL-0210
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Long non-coding RNA LINC00460 promotes proliferation and inhibits apoptosis of cervical cancer cells by targeting microRNA-503-5p(2020/8/1)
作者:Lin Lin, Bing Xin, Tao Jiang
期刊:MOLECULAR AND CELLULAR BIOCHEMISTRY
DOI:10.1007/s11010-020-03853-0.
影响因子 :4.300
引用产品:CL-0210
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USP21 regulates Hippo signaling to promote radioresistance by deubiquitinating FOXM1 in cervical cancer(2021/11/25)
作者:Zhengliang Li , Xiaojing Liu , Haizhou Yu
期刊:Human Cell
DOI:10.1007/s13577-021-00650-9
影响因子 :4.300
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OTX1 promotes tumorigenesis and progression of cervical cancer by regulating the Wnt signaling pathway(2022/09/29)
作者:Limin Zhou, Hongying Li, Dunlan Zhang
期刊:ONCOLOGY REPORTS
影响因子 :4.200
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Nocardia rubra cell-wall skeleton influences the development of cervical carcinoma by promoting the antitumor effect of macrophages and dendritic cells(2022/01/07)
作者:Siyang Zhang, Han Wang, Yisi Liu
期刊:Cancer Medicine
影响因子 :4.000
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HPV16 E6 promoting cervical cancer progression through down-regulation of miR-320a to increase TOP2A expression(2024/01/11)
作者:Jianing Zhang, Xiaohui Yu, Yi Guo
期刊:Cancer Medicine
影响因子 :4.000
引用产品:CL-0210
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APLN promotes the proliferation, migration, and glycolysis of cervical cancer through the PI3K/AKT/mTOR pathway(2024/03/30)
作者:Qi Wang, Bingyu Wang, Wenjing Zhang
期刊:ARCHIVES OF BIOCHEMISTRY AND BIOPHYSICS
影响因子 :3.900
引用产品:CL-0210
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Transient receptor potential channels’ genes forecast cervical cancer outcomes and illuminate its impact on tumor cells(2024/05/09)
作者:Shan Jiang, Xuefen Lin, Qiaoling Wu
期刊:Frontiers in Genetics
DOI:10.3389/fgene.2024.1391842
影响因子 :3.700
引用产品:CL-0210
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Characterization of the microenvironment in different immune-metabolism subtypes of cervical cancer with prognostic significance(2023/02/03)
作者:Wujiang Lai, Jinrong Liao, Xiaoxuan Li
期刊:Frontiers in Genetics
DOI:10.3389/fgene.2023.1067666
影响因子 :3.700
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Hypoxic tumor cell-derived small extracellular vesicle miR-152-3p promotes cervical cancer radioresistance through KLF15 protein(2023/11/07)
作者:Junying Zhou, Ningjing Lei, Wanjia Tian
期刊:Radiation Oncology
DOI:10.1186/s13014-023-02369-3
影响因子 :3.600
引用产品:CL-0210
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Inhibition of miR-574-5p suppresses cell growth and metastasis and enhances chemosensitivity by targeting RNA binding protein QKI in cervical cancer cells(2019/11/30)
作者:Rui Tong, Jingru Zhang, Chunyan Wang
期刊:Naunyn-Schmiedebergs Archives of Pharmacology
DOI:10.1007/s00210-019-01772-6
影响因子 :3.600
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USP7 promotes cervical cancer progression by stabilizing MTDH expression through deubiquitination(2024/04/16)
作者:Na Wang, Jing Xu, Yujing Wang
期刊:JOURNAL OF CANCER RESEARCH AND CLINICAL ONCOLOGY
DOI:10.1007/s00432-024-05710-9
影响因子 :3.600
引用产品:CL-0210
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hsa_circ_0119412 overexpression promotes cervical cancer progression by targeting miR-217 to upregulate anterior gradient 2(2022/03/11)
作者:Yumei Lv, Mingyi Wang, Mingli Chen
期刊:Journal Of Clinical Laboratory Analysis
影响因子 :3.124
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LINC00460 knockdown sensitizes cervical cancer to cisplatin by downregulating TGFBI(2023/12/28)
作者:Ping Tian, Yuanyuan Feng, Ling Tao
期刊:Chemical Biology & Drug Design
影响因子 :3.000
引用产品:CL-0210
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Transcription factor Dp-1 knockdown downregulates thymidine kinase 1 expression to protect against proliferation and epithelial-mesenchymal transition in cervical cancer(2023/09/16)
作者:Mei Wu, Mingji Ye
期刊:FUNCTIONAL & INTEGRATIVE GENOMICS
DOI:10.1007/s10142-023-01218-6
影响因子 :2.900
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Artesunate promotes cervical cancer cell apoptosis by regulating Bcl2 family molecules and reducing the mitochondrial membrane potential(2024/05/14)
作者:Qianying Zhang, Xing Li, Caiyi He
期刊:Oncology Letters
影响因子 :2.900
引用产品:CL-0210
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SHCBP1 contributes to the proliferation and self‑renewal of cervical cancer cells and activation of the NF‑κB signaling pathway through EIF5A(2023/04/21)
作者:Boya Deng, Ailin Li, Ying Zhu
期刊:Oncology Letters
影响因子 :2.900
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N6-methyladenosine methylation on FSCN1 mediated by METTL14/IGF2BP3 contributes to human papillomavirus type 16-infected cervical squamous cell carcinoma(2024/04/28)
作者:Qingqing Tian, Juqing Huang, Qin Zhang
期刊:CLINICAL AND EXPERIMENTAL PHARMACOLOGY AND PHYSIOLOGY
影响因子 :2.900
引用产品:CL-0210
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FBXO39 predicts poor prognosis and correlates with tumor progression in cervical squamous cell carcinoma(2022/08/28)
作者:Yanru Yang, Yun Zhao, Guorui Sun
期刊:PATHOLOGY RESEARCH AND PRACTICE
影响因子 :2.800
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TRIM21 promotes tumor progression and cancer stemness in cervical squamous cell carcinoma(2023/07/23)
作者:Yanru Yang, Saijie Zuo, Wenqing Li
期刊:PATHOLOGY RESEARCH AND PRACTICE
影响因子 :2.800
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CLCA2 overexpression suppresses epithelial-to-mesenchymal transition in cervical cancer cells through inactivation of ERK/JNK/p38-MAPK signaling pathways(2022/10/25)
作者:Wenhu Xin, Jian Zhang, Haibin Zhang
期刊:BMC Molecular and Cell Biology
DOI:10.1186/s12860-022-00440-7
影响因子 :2.800
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ZIC5 promotes aggressiveness and cancer stemness in cervical squamous cell carcinoma(2022/12/05)
作者:Qingge Jia, Junyang Song, Tianqi Xu
期刊:PATHOLOGY RESEARCH AND PRACTICE
影响因子 :2.800
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Mesencephalic astrocyte-derived neurotrophic factor inhibits cervical cancer progression via regulating macrophage phenotype(2024/05/12)
作者:Miaomiao Huang, Jingjing Hu, Yueran Chen
期刊:MOLECULAR BIOLOGY REPORTS
DOI:10.1007/s11033-024-09602-6
影响因子 :2.800
引用产品:CL-0210
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Relevance research between the expression of p16INK4a, Notch1, and hTERC genes: The development of HPV16‐positive cervical cancer(2020/1/24)
作者:Wenyan Huo, Shuaiyu Zhai, Yanbo Wang
期刊:Journal Of Clinical Laboratory Analysis
影响因子 :2.700
引用产品:CL-0210
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Circular RNA circ_0000119 promotes cervical cancer cell growth and migration via miR-433-3p/PAK2 axis(2023/08/04)
作者:Junxiao Zhang, Guanghua Chu, Lihua Zheng
期刊:JOURNAL OF APPLIED GENETICS
DOI:10.1007/s13353-023-00772-w
影响因子 :2.400
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Cellular Retinoic Acid Binding Protein 2 (CRABP2), Up-regulated by HPV E6/E7, Leads to Aberrant Activation of the Integrin β1/FAK/ERK Signaling Pathway and Aggravates the Malignant Phenotypes of Cervical Cancer(2023/11/24)
作者:Jiaxin Liu, Lu Tang, Wenzhu Chu
期刊:BIOCHEMICAL GENETICS
DOI:10.1007/s10528-023-10568-6
影响因子 :2.400
引用产品:CL-0210
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Circular RNA circVPRBP serves as a microRNA-106b-5p sponge to regulate proliferation and metastasis of cervical cancer cells via tripartite motif-containing protein 3(2022/08/10)
作者:Yingying Jiang, Wei Xu, Junzhen Tao
期刊:ANTI-CANCER DRUGS
DOI:10.1097/CAD.0000000000001335
影响因子 :2.300
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PLAC8 contributes to the malignant behaviors of cervical cancer cells by activating the SOX4-mediated AKT pathway(2023/01/05)
作者:Boya Deng, Siyang Zhang, Yingying Zhou
期刊:HISTOCHEMISTRY AND CELL BIOLOGY
DOI:10.1007/s00418-022-02175-0
影响因子 :2.300
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HIV gp120/Tat protein-induced epithelial–mesenchymal transition promotes the progression of cervical lesions(2023/11/19)
作者:Peizhi Wang, Baojun Yang, Huang Huang
期刊:AIDS Research and Therapy
DOI:10.1186/s12981-023-00577-1
影响因子 :2.200
引用产品:CL-0210
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LncRNA Opa interacting protein 5-antisense RNA 1 (OIP5-AS1) promotes the migration, invasion and epithelial-mesenchymal transition (EMT) through targeting miR-147a/insulin-like growth factor 1 receptor (IGF1R) pathway in cervical cancer tissues and c(2022/03/01)
作者:Limei Zhang, Yufei Cai, Chenchen Tian
期刊:Journal Of Obstetrics And Gynaecology Research
影响因子 :1.600
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