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产品概述
MEM培养基(Minimum Essential Medium)也称最低必需培养基、最小基本培养基或低限量Eagle培养基,由Harry Eagle在Eagle基本培养基(BEM)的基础上发展而来的,是一种最基本、适用范围最广的培养基,是动物细胞培养中最常用的培养基之一。MEM培养基仅含有12种必需氨基酸、谷氨酰胺和8种维生素,成分简单,主要用于贴壁细胞的培养,配方修改后也可用于其他类型细胞培养。
本产品含有多类细胞培养所需的氨基酸、维生素、无机盐等多种成分,但不含蛋白质、脂类或任何生长因子,故此产品需搭配血清或无血清添加物使用。
成分说明
形态 | 液体 |
浓度 | 1× |
规格 | 500mL |
pH | 7.2-7.4 |
平衡盐 | Earle’s 盐 |
NEAA | 无 |
L-谷氨酰胺 | 2 mM |
NaHCO3 | 2200 mg/L |
D-葡萄糖 | 1000 mg/L |
HEPES缓冲剂 | 无 |
酚红指示剂 | 10 mg/L |
储存条件 | 2-8℃,避光 |
运输条件 | 常温 |
有效期 | 18个月 |
注意事项
1、本产品经0.1 μm过滤除菌,使用时应注意无菌操作,避免污染;
2、为保持本产品的最佳使用效果,请勿进行冻融处理;
3、本产品仅用于科研或进一步研究使用,不用于诊断和治疗。
参考文献
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Single low-dose INC280-loaded theranostic nanoparticles achieve multirooted delivery for MET-targeted primary and liver metastatic NSCLC(2022/12/01)
作者:Yige Sun, Jie Yang, Yingbo Li
期刊:Molecular Cancer
DOI:10.1186/s12943-022-01681-y
影响因子 :37.300
引用产品:PM150411
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Cascade-Responsive “Oxidative Stress Amplifiers” Simultaneously Destroy Lysosomes and Co-Deliver CRISPR/Cas9 to Enhance Oxidative Damage in Tumor(2023/04/28)
作者:Yan Liang, Wenshuai Han, Chenlu Xu
期刊:ADVANCED FUNCTIONAL MATERIALS
影响因子 :19.000
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Rational development of multicomponent mRNA vaccine candidates against mpox(2023/03/31)
作者:Rongrong Zhang, Zhengjian Wang, Yilong Zhu
期刊:Emerging Microbes & Infections
DOI:10.1080/22221751.2023.2192815
影响因子 :13.200
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Injectable and photothermal antibacterial bacterial cellulose cryogel for rapid hemostasis and repair of irregular and deep skin wounds(2023/07/27)
作者:Shujun Cao, Kun Zhang, Qiujing Li
期刊:CARBOHYDRATE POLYMERS
DOI:10.1016/j.carbpol.2023.121239
影响因子 :11.200
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Immunomodulation of MiRNA-223-based nanoplatform for targeted therapy in retinopathy of prematurity(2022/09/12)
作者:Keke Huang, Zhiqing Lin, Yuanyuan Ge
期刊:JOURNAL OF CONTROLLED RELEASE
DOI:10.1016/j.jconrel.2022.08.006
影响因子 :10.800
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Peptidic heterodimer-based radiotracer targeting fibroblast activation protein and integrin αvβ3(2024/01/26)
作者:Kehuang Liu, Tao Jiang, Wanqian Rao
期刊:EUROPEAN JOURNAL OF NUCLEAR MEDICINE AND MOLECULAR IMAGING
DOI:10.1007/s00259-024-06623-y
影响因子 :9.100
引用产品:PM150411
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Effects of soil-extractable metals Cd and Ni from an e-waste dismantling site on human colonic epithelial cells Caco-2: Mechanisms and implications(2021/12/17)
作者:Jiaoyang Ma, Xinchen Bao, Wen Tian
期刊:Chemosphere
DOI:10.1016/j.chemosphere.2021.133361
影响因子 :8.943
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Empirical analysis of lead neurotoxicity mode of action and its application in health risk assessment(2024/03/15)
作者:Hehai Huang, Yanrong Lv, Qingfei Chen
期刊:ENVIRONMENTAL RESEARCH
DOI:10.1016/j.envres.2024.118708
影响因子 :8.300
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作者:Jiaojiao Jia, Zhihao Lin, Jilin Zhu
期刊:INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
DOI:10.1016/j.ijbiomac.2024.129668
影响因子 :8.200
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作者:Lei Fang, Yanling Hu, Zhihao Lin
期刊:INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
DOI:10.1016/j.ijbiomac.2024.130512
影响因子 :8.200
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作者:Shujun Cao, Qiujing Li, Shukun Zhang
期刊:INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
DOI:10.1016/j.ijbiomac.2022.09.172
影响因子 :8.200
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Acid-induced Poria cocos alkali-soluble polysaccharide hydrogel: Gelation behaviour, characteristics, and potential application in drug delivery(2023/04/06)
作者:Wan Li, Kexin Fang, Hao Yuan
期刊:INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
DOI:10.1016/j.ijbiomac.2023.124383
影响因子 :8.200
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Toxicity and oxidative stress of HepG2 and HL-7702 cells induced by PAH4 using oil as a carrier(2024/01/07)
作者:Guoyan Liu, Wanli Zhou, Xu Zhang
期刊:FOOD RESEARCH INTERNATIONAL
DOI:10.1016/j.foodres.2024.113988
影响因子 :8.100
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作者:Qinan Yin, Haodi Ma, Yirui Dong
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DOI:10.1186/s12967-024-04899-0
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Gut microbiome and metabolome to discover pathogenic bacteria and probiotics in ankylosing spondylitis(2024/04/01)
作者:Yupeng Lai, Wenli Tang, Xiao Luo
期刊:Frontiers in Immunology
DOI:10.3389/fimmu.2024.1369116
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Discovery of Novel 1,2,3,4-Tetrahydrobenzofuro[2,3-c]pyridine Histone Deacetylase Inhibitors for Efficient Treatment of Hepatocellular Carcinoma(2023/07/27)
作者:Zhencheng Lai, Hao Ni, Xueping Hu
期刊:JOURNAL OF MEDICINAL CHEMISTRY
DOI:10.1021/acs.jmedchem.3c01008
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SIRT1 activation by 2,3,5,6-tetramethylpyrazine alleviates neuroinflammation via inhibiting M1 microglia polarization(2023/08/04)
作者:Yu Chen, Fu Peng, Chao Yang
期刊:Frontiers in Immunology
DOI:10.3389/fimmu.2023.1206513
影响因子 :7.300
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Identification and validation of chemokine system-related genes in idiopathic pulmonary fibrosis(2023/04/14)
作者:Tianming Zhao, Xu Wu, Xuelei Zhao
期刊:Frontiers in Immunology
DOI:10.3389/fimmu.2023.1159856
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Therapeutic effects against high-grade glioblastoma mediated by engineered induced neural stem cells combined with GD2-specific CAR-NK(2023/07/07)
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DOI:10.1007/s13402-023-00842-5
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DOI:10.1016/j.bbadis.2024.167050
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作者:Luyao Gao, Xiaoduo Zhao, Yunlei Zhang
期刊:BIOMACROMOLECULES
DOI:10.1021/acs.biomac.2c00640
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作者:Meilian Yang, Shengpeng Su, Shuzhen Cheng
期刊:Food & Function
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期刊:LAB ON A CHIP
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Super-resolution imaging of folate receptor alpha on cell membranes using peptide-based probes(2023/10/07)
作者:Yingying Jing, Lilin Huang, Zufu Dong
期刊:TALANTA
DOI:10.1016/j.talanta.2023.125286
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Construction of multifunctional targeted nano-prodrugs based on PAMAM dendrimers for tumor therapy(2023/10/10)
作者:Yuan Gu, Yanjun Cai, Yige Kou
期刊:EUROPEAN POLYMER JOURNAL
DOI:10.1016/j.eurpolymj.2023.112486
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A1BG-AS1 promotes the biological functions of osteosarcoma cells via regulating the microRNA-148a-3p/USP22 axis and stabilizing the expression of SIRT1 through deubiquitinase function(2023/10/03)
作者:Xiuxin Han, Mengfan Yin, Chen Gong
期刊:EXPERT OPINION ON THERAPEUTIC TARGETS
DOI:10.1080/14728222.2023.2263908
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DOI:10.1021/acsbiomaterials.3c01353
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期刊:Frontiers in Oncology
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DOI:10.1016/j.bioorg.2024.107375
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DOI:10.1016/j.labinv.2022.100019
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DOI:10.1016/j.jddst.2023.104960
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DOI:10.1016/j.cellsig.2024.111088
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Antioxidant Effects of Roasted Licorice in a Zebrafish Model and Its Mechanisms(2022/11/10)
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DOI:10.1016/j.aquaculture.2024.740975
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DOI:10.1016/j.taap.2022.116232
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DOI:10.1016/j.phytochem.2024.114018
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Increased extracellular matrix stiffness regulates myofibroblast transformation through induction of autophagy-mediated Kindlin-2 cytoplasmic translocation(2024/02/10)
作者:Zhihan Zhao, Wuyue han, Guotao Huang
期刊:EXPERIMENTAL CELL RESEARCH
DOI:10.1016/j.yexcr.2024.113974
影响因子 :3.700
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Improved Antioxidant Capacity of Akebia trifoliata Fruit Inoculated Fermentation by Plantilactobacillus plantarum, Mechanism of Anti-Oxidative Stress through Network Pharmacology, Molecular Docking and Experiment Validation by HepG2 Cells(2023/04/29)
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期刊:Fermentation-Basel
DOI:10.3390/fermentation9050432
影响因子 :3.700
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FDX1 expression predicts favourable prognosis in clear cell renal cell carcinoma identified by bioinformatics and tissue microarray analysis(2022/09/16)
作者:Xing Huang, Tao Wang, Jiali Ye
期刊:Frontiers in Genetics
影响因子 :3.700
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Long non-coding RNA SNHG7 upregulates FGF9 to alleviate oxygen and glucose deprivation-induced neuron cell injury in a miR-134-5p-dependent manner(2021/10/18)
作者:Wei Sun, Lu Sun,Xiaopeng Sun
期刊:Metabolic Brain Disease
DOI:10.1007/s11011-021-00852-y
影响因子 :3.655
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hsa_circ_0020378 regulating miR-339-3p/COL1A1 promotes osteosarcoma progression(2023/11/18)
作者:Liang Liu, Wenze Huang
期刊:CANCER BIOLOGY & THERAPY
DOI:10.1080/15384047.2023.2274120
影响因子 :3.600
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Aldolase A promotes cervical cancer cell radioresistance by regulating the glycolysis and DNA damage after irradiation(2023/11/27)
作者:Junying Zhou, Ningjing Lei, Bo Qin
期刊:CANCER BIOLOGY & THERAPY
DOI:10.1080/15384047.2023.2287128
影响因子 :3.600
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Cytoprotective effects of C1s enzyme in macrophages in atherosclerosis mediated through the LRP5 and Wnt/β-catenin pathway(2024/01/12)
作者:Dong Yuan, Zhipeng Zheng, Cheng Shen
期刊:MOLECULAR IMMUNOLOGY
DOI:10.1016/j.molimm.2024.01.002
影响因子 :3.600
引用产品:PM150411
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Diverse cell death patterns-related signature for predicting prognosis and drug sensitivity of osteosarcoma patients(2023/10/20)
作者:Yanbin Shi, Song Wu, Xiaolin Zhang
期刊:JOURNAL OF GENE MEDICINE
DOI:10.1002/jgm.3613
影响因子 :3.500
引用产品:PM150411
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Identify CTBP1-DT as an immunological biomarker that promotes lipid synthesis and apoptosis resistance in KIRC(2024/03/21)
作者:Haolin Li, Mintian Fei, Yi Zhang
期刊:GENE
DOI:10.1016/j.gene.2024.148403
影响因子 :3.500
引用产品:PM150411
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Integrative analysis of transcriptome and metabolome provide new insights into mechanisms of Capilliposide A against cisplatin-induced nephrotoxicity(2023/10/27)
作者:Jiaxi Fang, Luping Wang, Di Zhang
期刊:JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS
DOI:10.1016/j.jpba.2023.115814
影响因子 :3.400
引用产品:PM150411
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G protein‐coupled bile acid receptor 1 reduced hepatic immune response and inhibited NFκB, PI3K/AKT and PKC/P38 MAPK signaling pathway in hybrid grouper(2023/09/16)
作者:Jia Xu, Junming Cao, Beiping Tan
期刊:JOURNAL OF ANIMAL SCIENCE
影响因子 :3.300
引用产品:PM150411
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The effect of resolvin D1 on bone regeneration in a rat calvarial defect model(2022/08/18)
作者:Xiaofeng Jiang, Jing Liu, Si Li
期刊:Journal of Tissue Engineering and Regenerative Medicine
影响因子 :3.300
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V-set and immunoglobulin domain containing 4 inhibits oxidative stress, mitochondrial dysfunction, and inflammation to attenuate Parkinsons disease progression by activating the JAK2/STAT3 pathway(2024/04/17)
作者:Pingping Cai, Junmei Wang, Jiangtao Xu
期刊:JOURNAL OF NEUROIMMUNOLOGY
DOI:10.1016/j.jneuroim.2024.578345
影响因子 :3.300
引用产品:PM150411
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Integrated bioinformatics analysis for the identification of idiopathic pulmonary fibrosis–related genes and potential therapeutic drugs(2023/10/04)
作者:Zhang Zhenzhen, Guan Qingzhou, Tian Yange
期刊:BMC Pulmonary Medicine
DOI:10.1186/s12890-023-02678-z
影响因子 :3.100
引用产品:PM150411
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Matrine inhibits cell proliferation, invasion, and stem cell formation in hepatocellular carcinoma by regulating the ELAVL1/RBM3-mediated Wnt/β-catenin signaling pathway(2024/01/23)
作者:Chunhua Song, Hongwei Li, Zhengchun Huang
期刊:Chemical Biology & Drug Design
影响因子 :3.000
引用产品:PM150411
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CircZNF644 aggravates lipopolysaccharide-induced HK-2 cell impairment via the miR-140-5p/MLKL axis(2022/08/17)
作者:Jing Xing, Songtao Fan, Hongyang Liu
期刊:JOURNAL OF BIOENERGETICS AND BIOMEMBRANES
DOI:10.1007/s10863-022-09946-3
影响因子 :3.000
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Proliferation Inhibitory Activity of Quinones from Blaps rynchopetera Defense Secretion on Colorectal Tumor Cells(2023/04/18)
作者:Xiaoli Qian, Di Meng, Heng Liu
期刊:Chinese Journal of Integrative Medicine
影响因子 :2.900
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Circ_0026579 knockdown ameliorates lipopolysaccharide-induced human lung fibroblast cell injury by regulating CXCR1 via miR-370-3p(2023/10/02)
作者:Yuanhong Zhuang, Yuyun Yang, Lihong Peng
期刊:CLINICAL AND EXPERIMENTAL PHARMACOLOGY AND PHYSIOLOGY
影响因子 :2.900
引用产品:PM150411
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ARHGAP44-mediated regulation of the p53/C-myc/Cyclin D1 pathway in modulating the malignant biological behavior of osteosarcoma cells(2023/11/29)
作者:Shizhe Li, Jiancheng Xue, He Zhang
期刊:Journal of Orthopaedic Surgery and Research
DOI:10.1186/s13018-023-04406-z
影响因子 :2.600
引用产品:PM150411
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Knockdown of circ-Gatad1 alleviates LPS induced HK2 cell injury via targeting miR-22-3p/TRPM7 axis in septic acute kidney(2024/03/05)
作者:Pan Zhang, Enwei Guo, Limin Xu
期刊:BMC Nephrology
DOI:10.1186/s12882-024-03513-1
影响因子 :2.300
引用产品:PM150411
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The role of CRYAB in tumor prognosis and immune infiltration: A Pan-cancer analysis(2023/01/13)
作者:Lang Cheng, Xiong Zou, Jiawei Wang
期刊:Frontiers in Surgery
DOI:10.3389/fsurg.2022.1117307
影响因子 :1.800
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