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产品概述
Ham's F-12营养混合物（Ham's F-12 Nutrient Mixture）是由Ham在1969年以Ham's F-10营养混合物为基础设计而成的，最初用于CHO细胞的无血清培养。Ham's F-12常作为无血清培养的基础培养液，在低血清含量下时，特别适合单细胞培养和克隆化培养，添加血清后也广泛应用于癌细胞和原代细胞的培养，如大鼠肝细胞、大鼠前列腺上皮细胞、软骨细胞、大鼠成肌细胞、鸡胚胎细胞等。此外，Ham's F-12与DMEM等体积混合后，得到的DMEM/F12培养基营养更为丰富，使用更为广泛。
本产品含有多类细胞培养所需的氨基酸、维生素、无机盐等多种成分，但不含蛋白质、脂类或任何生长因子，故此产品需搭配血清或无血清添加物使用。
成分说明
形态 | 液体 |
浓度 | 1× |
规格 | 500mL |
pH | 7.2-7.4 |
L-谷氨酰胺 | 1 mM |
NaHCO3 | 1176 mg/L |
D-葡萄糖 | 1802 mg/L |
HEPES缓冲剂 | 无 |
酚红指示剂 | 1.2 mg/L |
储存条件 | 2-8℃，避光 |
运输条件 | 常温 |
有效期 | 18个月 |
注意事项
1、本产品经0.1 μm过滤除菌，使用时应注意无菌操作，避免污染；
2、为保持本产品的最佳使用效果，请勿进行冻融处理；
3、本产品仅用于科研或进一步研究使用，不用于诊断和治疗。
参考文献
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TEAD4 functions as a prognostic biomarker and triggers EMT via PI3K/AKT pathway in bladder cancer(2022/05/17)
作者：Ming Chi, Jiao Liu, Chenxue Mei
期刊：JOURNAL OF EXPERIMENTAL & CLINICAL CANCER RESEARCH
DOI：10.1186/s13046-022-02377-3
影响因子 ：12.658
-
The alternation of halobenzoquinone disinfection byproduct on toxicogenomics of DNA damage and repair in uroepithelial cells(2023/12/22)
作者：Xu Zhang, Lifang Liu, Jun Wang
期刊：ENVIRONMENT INTERNATIONAL
DOI：10.1016/j.envint.2023.108407
影响因子 ：11.800
引用产品：PM150810
-
A novel 450-nm laser-mediated sinoporphyrin sodium-based photodynamic therapy induces autophagic cell death in gastric cancer through regulation of the ROS/PI3K/Akt/mTOR signaling pathway(2022/12/08)
作者：Xing Li, Lijiang Gu, Yuhang Chen
期刊：BMC Medicine
DOI：10.1186/s12916-022-02676-8
影响因子 ：9.300
引用产品：PM150810
-
Dual PI3K/mTOR inhibitor PF-04979064 regulates tumor growth in gastric cancer and enhances drug sensitivity of gastric cancer cells to 5-FU(2023/12/29)
作者：Ziyuan Zhong, Tengkai Wang, Ruochen Zang
期刊：BIOMEDICINE & PHARMACOTHERAPY
DOI：10.1016/j.biopha.2023.116086
影响因子 ：7.500
引用产品：PM150810
-
Quercetin induces ferroptosis in gastric cancer cells by targeting SLC1A5 and regulating the p-Camk2/p-DRP1 and NRF2/GPX4 Axes(2024/01/06)
作者：Lixian Ding, Shuwei Dang, Mingjun Sun
期刊：FREE RADICAL BIOLOGY AND MEDICINE
DOI：10.1016/j.freeradbiomed.2024.01.002
影响因子 ：7.400
引用产品：PM150810
-
Synergistic protection of quercetin and lycopene against oxidative stress via SIRT1-Nox4-ROS axis in HUVEC cells(2022/10/18)
作者：Xuan Chen, Liufeng Zheng, Bing Zhang
期刊：Current Research in Food Science
DOI：10.1016/j.crfs.2022.10.018
影响因子 ：6.300
引用产品：PM150810
-
Asiaticoside promoted ferroptosis and suppressed immune escape in gastric cancer cells by downregulating the Wnt/β-catenin pathway(2024/05/10)
作者：Chenmin Ye, Zhichao Yao, Yaoyao Wang
期刊：INTERNATIONAL IMMUNOPHARMACOLOGY
DOI：10.1016/j.intimp.2024.112175
影响因子 ：5.600
引用产品：PM150810
-
KIRREL promotes the proliferation of gastric cancer cells and angiogenesis through the PI3K/AKT/mTOR pathway(2023/11/01)
作者：Tao Wang, Shuo Chen, Ziliang Wang
期刊：JOURNAL OF CELLULAR AND MOLECULAR MEDICINE
影响因子 ：5.300
引用产品：PM150810
-
Isolation, Identification and Chemical Modification of Bufadienolides from Bufo melanostictus Schneider and Their Cytotoxic Activities against Prostate Cancer Cells(2024/03/31)
作者：Qingmei Ye, Rong Lin, Zeping Chen
期刊：MOLECULES
影响因子 ：4.600
引用产品：PM150810
-
FTIR microspectroscopic study of gastric cancer AGS cells apoptosis induced by As2O3(2024/02/08)
作者：Chao Li, Jie Shi, Yongan Wang
期刊：SPECTROCHIMICA ACTA PART A-MOLECULAR AND BIOMOLECULAR SPECTROSCOPY
影响因子 ：4.400
引用产品：PM150810
-
Cancer-associated fibroblast expression of glutamine fructose-6-phosphate aminotransferase 2 (GFPT2) is a prognostic marker in gastric cancer(2023/07/03)
作者：Shuo Yang, Guoli Li, Xin Yin
期刊：Journal of Pathology Clinical Research
DOI：10.1002/cjp2.333
影响因子 ：4.100
-
Irradiation Induces Gasdermin E-Triggered Tumor Immunity to Inhibit Esophageal Carcinoma Cell Survival(2023/11/29)
作者：Bingxu Tan, Nana Wang, Shengsi Yang
期刊：ACS Omega
影响因子 ：4.100
引用产品：PM150810
-
Melatonin suppresses Akt/mTOR/S6K activity, induces cell apoptosis, and synergistically inhibits cell growth with sunitinib in renal carcinoma cells via reversing Warburg effect(2023/08/29)
作者：Kaihua Xue, Yifan Jiang, Jiyu Bai
期刊：REDOX REPORT
DOI：10.1080/13510002.2023.2251234
影响因子 ：3.800
引用产品：PM150810
-
YTHDF2 promotes gastric cancer progression and enhances chemoradiotherapy resistance(2024/04/14)
作者：Jian Yang, Yawen Chen, Yang He
期刊：DRUG DEVELOPMENT RESEARCH
影响因子 ：3.800
引用产品：PM150810
-
Circular RNA CircSLC22A23 Promotes Gastric Cancer Progression by Activating HNRNPU Expression(2024/02/24)
作者：Xinxin Wu, Chunli Cao, Zhe Li
期刊：DIGESTIVE DISEASES AND SCIENCES
DOI：10.1007/s10620-024-08291-2
影响因子 ：3.100
引用产品：PM150810
-
Chrysophanol induces apoptosis and ferroptosis of gastric cancer cells by targeted regulation of mTOR(2024/01/07)
作者：Sujuan Xi, Wei Ding, Danping Weng
期刊：Chemical Biology & Drug Design
影响因子 ：3.000
引用产品：PM150810
-
LINC01268 promotes epithelial-mesenchymal transition, invasion and metastasis of gastric cancer via the PI3K/Akt signaling pathway and targeting MARCKS(2023/08/15)
作者：Linghan Tang, Pengcheng Ye, Lin Yao
期刊：World Journal of Gastrointestinal Oncology
影响因子 ：3.000
-
Long non-coding RNA GATA6-AS1 is mediated by N6-methyladenosine methylation and inhibits the proliferation and metastasis of gastric cancer(2024/03/15)
作者：Junjie Shen, Minchang Li, Shaoqi Tian
期刊：World Journal of Gastrointestinal Oncology
影响因子 ：3.000
引用产品：PM150810
-
A prognostic marker LTBP1 is associated with epithelial mesenchymal transition and can promote the progression of gastric cancer(2024/02/15)
作者：Xinju Jiang, Shengjie Yin, Xin Yin
期刊：FUNCTIONAL & INTEGRATIVE GENOMICS
DOI：10.1007/s10142-024-01311-4
影响因子 ：2.900
引用产品：PM150810
-
Rosiglitazone attenuates high glucose-induced proliferation, inflammation, oxidative stress and extracellular matrix accumulation in mouse mesangial cells through the Gm26917/miR-185-5p pathway(2021/03/30)
作者：Dongbo Zhao, Junli Guo, Lingping Liu
期刊：Endocrine Journal
影响因子 ：2.860
-
Oxymatrine induces apoptosis in non-small cell lung cancer cells by downregulating TRIM46(2024/05/23)
作者：Yi Zhong, Biaobiao Luo, Min Hong
期刊：TOXICON
DOI：10.1016/j.toxicon.2024.107773
影响因子 ：2.800
引用产品：PM150810
-
Upregulation of KIF18B facilitates malignant phenotype of esophageal squamous cell carcinoma by activating CDCA8/mTORC1 pathway(2022/09/09)
作者：Jiangliu Xie, Bo Wang, Wenjie Luo
期刊：JOURNAL OF CLINICAL LABORATORY ANALYSIS
影响因子 ：2.700
-
Comprehensive analysis of COMMD10 as a novel prognostic biomarker for gastric cancer(2023/03/09)
作者：Wenfang Zhao, Jiahui Lin, Sha Cheng
期刊：PeerJ
影响因子 ：2.700
-
E2F1 induced neuroblastoma cell migration and invasion via activation of CENPE/FOXM1 signaling pathway(2022/10/03)
作者：Jing Wang, Wang Dai, Ming Zhang
期刊：INTERNATIONAL JOURNAL OF NEUROSCIENCE
DOI：10.1080/00207454.2022.2126772
影响因子 ：2.590
引用产品：PM150810
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Knockdown circZNF131 Inhibits Cell Progression and Glycolysis in Gastric Cancer Through miR-186-5p/PFKFB2 Axis(2022/01/21)
作者：Xingjie Shen, Xiaoyan Zhu, Peixin Hu
期刊：Biochemical Genetics
DOI：10.1007/s10528-021-10165-5
影响因子 ：2.400
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