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大鼠骨髓间充质干细胞
Rat Bone Marrow Mesenchymal Stem Cells
货号：CP-R131
规格：5×10⁵Cells/T25培养瓶
价格： ￥3380
培养基货号： CM-R131
产品概述
大鼠骨髓间充质干细胞
Cat NO.: CP-R131
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产品名称：大鼠骨髓间充质干细胞
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英文简称：BMSCs,MSCs
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组织来源：骨髓
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产品规格：5×105cells/T25细胞培养瓶
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细胞简介：
大鼠骨髓间充质干细胞(BMSC)分离自骨髓；骨髓是机体的造血组织，位于身体的许多骨骼内。成年动物的骨髓分两种：红骨髓和黄骨髓。红骨髓能制造红细胞、血小板和各种白细胞。血小板有止血作用，白细胞能杀灭与抑制各种病原体，包括细菌、病毒等；某些淋巴细胞能制造抗体。因此，骨髓不但是造血器官，它还是重要的免疫器官。骨髓是存在于长骨(如肱骨、股骨)的骨髓腔和扁平骨(如髂骨)的稀松骨质间的网眼中，是一种海绵状的组织，能产生血细胞的骨髓略呈红色，称为红骨髓。出生时，红骨髓充满全身骨髓腔，随着年龄增大，脂肪细胞增多，相当部分红骨髓被黄骨髓取代，最后几乎只有扁平骨骨髓腔中有红骨髓。骨髓基质系统内存在的骨髓间充质干细胞是一种除造血干细胞以外的、具有高度自我更新能力和多向分化潜能的干细胞，可以向骨、软骨、肌组织、皮肤、脂肪、神经等多种组织分化，因此可以作为组织工程中的种子细胞。在骨髓中，BMSC占骨髓有核细胞总数的0.001%-0.1%，含量极低。骨髓间充质干细胞的体外培养条件要求较高，在培养过程中，受贴壁时间、种植密度、血清含量、培养温度和培养基pH值等条件的影响。
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方法简介：
普诺赛实验室分离的大鼠骨髓间充质干细胞采用冲洗骨髓、密度梯度离心、差速贴壁法结合培养基筛选制备而来，细胞总量约为5×10⁵cells/瓶。
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质量检测：
普诺赛实验室分离的大鼠骨髓间充质干细胞经CD90或CD44免疫荧光鉴定，纯度可达90%以上，且不含有HIV-1、HBV、HCV、支原体、细菌、酵母和真菌等。
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培养信息：
包被条件 培养基 含FBS、生长添加剂、Penicillin、Streptomycin等 产品货号 CM-R131 换液频率 每2-3天换液一次 生长特性 贴壁 细胞形态 成纤维细胞样 传代特性 可传5代左右；3代以内状态最佳 消化液 0.25%胰蛋白酶 培养条件 气相：空气，95%；CO2，5% 大鼠骨髓间充质干细胞体外培养周期有限；建议使用普诺赛配套的专用生长培养基及正确的操作方法来培养，以此保证该细胞的最佳培养状态。
参考文献
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Selenoprotein-Regulated Hydrogel for Ultrasound-Controlled Microenvironment Remodeling to Promote Bone Defect Repair(2024/01/14)
作者：Renhao Xu, Yuanyuan You, Wenyi Zheng
期刊：ADVANCED FUNCTIONAL MATERIALS
影响因子 ：19.000
引用产品：CP-R131
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Injectable and in situ foaming shape-adaptive porous Bio-based polyurethane scaffold used for cartilage regeneration(2024/05/13)
作者：Abudureheman Bahatibieke, Shuai Wei, Han Feng
期刊：Bioactive Materials
DOI：10.1016/j.bioactmat.2024.03.012
影响因子 ：18.900
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An injectable and coagulation-independent Tetra-PEG hydrogel bioadhesive for post-extraction hemostasis and alveolar bone regeneration(2024/03/19)
作者：Gang He, Yiwen Xian, Huajun Lin
期刊：Bioactive Materials
DOI：10.1016/j.bioactmat.2024.03.015
影响因子 ：18.900
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An anti-infection and biodegradable TFRD-loaded porous scaffold promotes bone regeneration in segmental bone defects -experimental studies(2024/03/19)
作者：Haixiong Lin, Zige Li, Zhenze Xie
期刊：International Journal of Surgery
DOI：10.1097/JS9.0000000000001291
影响因子 ：15.300
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Electrospun photocrosslinkable hydrogel fibrous membrane with metal ion trapping capability as an artificial periosteum to promote bone regeneration(2023/12/22)
作者：Xi He, Ruideng Wang, Li Wang
期刊：COMPOSITES PART B-ENGINEERING
DOI：10.1016/j.compositesb.2023.111147
影响因子 ：13.100
引用产品：CP-R131
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Natural micropatterned fish scales combing direct osteogenesis and osteoimmunomodulatory functions for enhancing bone regeneration(2023/02/13)
作者：Di Qin, Xinguo You, Haonan Wang
期刊：COMPOSITES PART B-ENGINEERING
DOI：10.1016/j.compositesb.2023.110620
影响因子 ：13.100
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In situ preparation of nano cone-like structures on 3D printed titanium alloy implants via one-step femtosecond laser manufacturing for better osseointegration, anti-corrosion, and anti-fatigue(2024/04/25)
作者：Yazhou Hu, Hongshui Wang, Donghui Wang
期刊：JOURNAL OF MATERIALS SCIENCE & TECHNOLOGY
DOI：10.1016/j.jmst.2024.03.070
影响因子 ：10.900
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Inducing in situ M2 macrophage polarization to promote the repair of bone defects via scaffold-mediated sustained delivery of luteolin(2023/12/18)
作者：Yan Hu, Lixi Tang, Zheng Wang
期刊：JOURNAL OF CONTROLLED RELEASE
DOI：10.1016/j.jconrel.2023.11.015
影响因子 ：10.800
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Cu–Sr Bilayer Bioactive Glass Nanoparticles/Polydopamine Functionalized Polyetheretherketone Enhances Osteogenic Activity and Prevents Implant-Associated Infections through Spatiotemporal Immunomodulation(2023/09/18)
作者：Yuezhou Wu, Shicheng Huo, Shu Liu
期刊：Advanced Healthcare Materials
影响因子 ：10.000
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Functionalized 3D Hydroxyapatite Scaffold by Fusion Peptides-Mediated Small Extracellular Vesicles of Stem Cells for Bone Tissue Regeneration(2024/01/12)
作者：Shiqing Ma, Beibei Ma, Yilin Yang
期刊：ACS Applied Materials & Interfaces
影响因子 ：9.500
引用产品：CP-R131
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Titanium dioxide nanotubes increase purinergic receptor P2Y6 expression and activate its downstream PKCα-ERK1/2 pathway in bone marrow mesenchymal stem cells under osteogenic induction(2022/11/26)
作者：Chen Wang, Yanchang Liu, Xuyang Hu, Xianbo Shang, Sai Ma, Huihui Guo, Xiao Ma, Dechao Cai, Zhongyao Hu, Yao Zhao, Yakun Zhu, Zhengpin Cao, Haoran Yu, Wendan Cheng
期刊：Acta Biomaterialia
DOI：10.1016/j.actbio.2022.11.045
影响因子 ：9.300
引用产品：CP-R131
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Bi-lineage inducible and immunoregulatory electrospun fibers scaffolds for synchronous regeneration of tendon-to-bone interface(2023/07/26)
作者：Haihan Gao, Liren Wang, Zhiqi Lin
期刊：Materials Today Bio
DOI：10.1016/j.mtbio.2023.100749
影响因子 ：8.200
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2D silicene nanosheets-loaded coating for combating implant-associated infection(2023/10/21)
作者：Junkai Zeng, Changjiang Gu, Feihui Zeng
期刊：INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
DOI：10.1016/j.ijbiomac.2023.127585
影响因子 ：8.200
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Multifunctional tannic acid-based nanocomposite methacrylated silk fibroin hydrogel with the ability to scavenge reactive oxygen species and reduce inflammation for bone regeneration(2024/04/03)
作者：Ruideng Wang, Xi He, Shilong Su
期刊：INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
DOI：10.1016/j.ijbiomac.2024.131357
影响因子 ：8.200
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3D printed-electrospun PCL/Hydroxyapatite/MWCNTs scaffolds for the repair of subchondral bone(2022/12/14)
作者：Yanyan Cao, Lei Sun, Zixian Liu
期刊：Regenerative Biomaterials
影响因子 ：6.700
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Research on the osteogenesis and biosafety of ECM–Loaded 3D–Printed Gel/SA/58sBG scaffolds(2022/08/17)
作者：Guozhong Tan, Rongfeng Chen, Xinran Tu
期刊：Frontiers in Bioengineering and Biotechnology
影响因子 ：6.064
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Coatless modification of 3D-printed Ti6Al4V implants through tailored Cu ion implantation combined with UV photofunctionalization to enhance cell attachment, osteogenesis and angiogenesis(2024/04/06)
作者：Jiedong Wang, Zehao Jing, Chuan Yin
期刊：COLLOIDS AND SURFACES B-BIOINTERFACES
DOI：10.1016/j.colsurfb.2024.113891
影响因子 ：5.800
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Bioinspired strontium magnesium phosphate cement prepared utilizing the precursor method for bone tissue engineering(2023/02/03)
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期刊：Frontiers in Bioengineering and Biotechnology
DOI：10.3389/fbioe.2023.1142095
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Preparation and In Vitro Characterization of Gelatin Methacrylate for Corneal Tissue Engineering(2021/10/19)
作者：Yayun Yan, Yanyan Cao, Rong Cheng
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DOI：10.1007/s13770-021-00393-6
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Osteogenic Effect of tsRNA-10277-Loaded Exosome Derived from Bone Mesenchymal Stem Cells on Steroid-Induced Osteonecrosis of the Femoral Head(2022/10/31)
作者：Shanhong Fang, Tianmin He, Jiarun Jiang
期刊：Drug Design Development and Therapy
影响因子 ：4.319
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In situ fabrication of an anisotropic double-layer hydrogel as a bio-scaffold for repairing articular cartilage and subchondral bone injuries(2023/11/30)
作者：Xiaotian Yu, Zhantao Deng, Han Li
期刊：RSC Advances
影响因子 ：3.900
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Exploring the mechanism of Astragali radix for promoting osteogenic differentiation based on network pharmacology, molecular docking, and experimental validation(2023/09/10)
作者：Zenghui Tian, Yingying Li, Xiaoying Wang
期刊：Chemical Biology & Drug Design
影响因子 ：3.000
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Exosome-shuttled MYCBPAP from bone marrow mesenchymal stem cells regulates synaptic remodeling and ameliorates ischemic stroke in rats(2023/07/07)
作者：Qiuyue Yan, Yong Yin, Xuechun Li
期刊：JOURNAL OF CHEMICAL NEUROANATOMY
DOI：10.1016/j.jchemneu.2023.102309
影响因子 ：2.800
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A novel natural-derived tilapia skin collagen mineralized with hydroxyapatite as a potential bone-grafting scaffold:(2022/03/29)
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A New Method Regulates Bone Fracture Tissue Exosome lncRNA-mRNA to Promote Mesenchymal Stem Cell Proliferation and Migration(2023/11/14)
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期刊：INJURY-INTERNATIONAL JOURNAL OF THE CARE OF THE INJURED
DOI：10.1016/j.injury.2023.111210
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Bone mesenchymal stem cell-derived exosomal miR-26a-3p promotes autophagy to attenuate LPS-induced apoptosis and inflammation in pulmonary microvascular endothelial cells(2024/02/29)
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期刊：CELLULAR AND MOLECULAR BIOLOGY
影响因子 ：1.600
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