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产品概述
名称 | A-431 (人表皮癌细胞) (STR鉴定正确) |
别称 | A431; A431/P |
种属 | 人 |
生长特性 | 贴壁细胞 |
细胞形态 | 上皮细胞样 |
冻存条件 | 冻存液:55% 基础培养基+40%FBS+5%DMSO 温度:液氮 |
培养方案A(默认) |
培养条件:
气相:空气,95%;CO2,5%, 温度:37℃
|
推荐传代比例 | 1:3-1:4 |
推荐换液频率 | 2-3次/周 |
注意事项 | 1.细胞成片生长,生长速度缓慢;2.细胞贴壁很牢、较难消化;如有必要,可分次消化。 |
背景描述 | 皮肤癌细胞株A-431源自一位85岁女性,是由D·J·Giard等人建立的一系列细胞株中的一株。A-431细胞在抗胸腺细胞球蛋白、血清处理的NIH瑞士小鼠中形成类似于恶性黑素瘤快速增长的皮下肿瘤,在普通成纤维细胞和琼脂上形成克隆。A-431细胞是一个超三倍体人细胞株。 |
年龄(性别) | 女性;85岁 |
组织来源 | 皮肤;上皮;皮肤癌 |
细胞类型 | 肿瘤细胞 |
肿瘤类型 | 其它肿瘤细胞 |
生物安全等级 | BSL-1 |
倍增时间 | ~80-100 hours |
致瘤性 | Yes, forms rapidly growing subcutaneous tumors in immunosuppressed mice and colonies in soft agar. |
保藏机构 | ATCC; CRL-1555 ATCC; CRL-7907BCRC; 60161 BCRJ; 0032 DSMZ; ACC-91 ECACC; 85090402 |
STR鉴定
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STR位点信息
Amelogenin X CSF1PO 11,12 D2S1338 17,20 D3S1358 14 D5S818 12,13 D7S820 10 D8S1179 13 D13S317 9,13 D16S539 12,14 D18S51 13,17 D19S433 15,15.2 D21S11 28,30 FGA 20 PentaD 9,11 PentaE 12,13 TH01 9 TPOX 11 vWA 15,17 D6S1043 12,18 D12S391 18,23 D2S441 11.3,15 -
STR鉴定图
参考文献
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Exosome-tuned MOF signal amplifier boosting tumor exosome phenotyping with high-affinity nanostars(2023/11/11)
作者:Xiaojie Qin, Binqi Wei, Yuanhang Xiang
期刊:BIOSENSORS & BIOELECTRONICS
DOI:10.1016/j.bios.2023.115828
影响因子 :12.600
引用产品:CL-0015
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Near-Infrared Light-Activated Oxygen Generator a Multidynamic Photo-Nanoplatform for Effective Anti-Cutaneous Squamous Cell Carcinoma Treatment(2022/11/28)
作者:Xin Zhang, Xiangbo Bu, Wenyu Jia, Yu Ying, Shanrong Lv, Guan Jiang
期刊:International Journal of Nanomedicine
影响因子 :8.000
引用产品:CL-0015
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Self-Assembly of Erlotinib-Platinum(II) Complexes for Epidermal Growth Factor Receptor-Targeted Photodynamic Therapy(2024/01/06)
作者:Haobing Wang, Yidan Lai, Dan Li
期刊:JOURNAL OF MEDICINAL CHEMISTRY
DOI:10.1021/acs.jmedchem.3c01889
影响因子 :7.300
引用产品:CL-0015
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Novel 4-anilinoquinazoline derivatives featuring an 1-adamantylmoiety as potent EGFR inhibitors with enhanced activity againstNSCLC cell lines(2016/01/26)
作者:Haiqing Yu, Yanxia Li, Yang Ge
期刊:European Journal of Medicinal Chemistry
DOI:DOI: 10.1016/j.ejmech.2016.01.045
影响因子 :6.700
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Regulatory role of methionine enkephalin in myeloid-derived suppressor cells and macrophages in human cutaneous squamous cell carcinoma(2021/07/23)
作者:Xueli Bai, Fengping Shan, Na Qu
期刊:International Immunopharmacology
DOI:10.1016/j.intimp.2021.107996
影响因子 :5.600
引用产品:CL-0015
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ATP Citrate Lyase is a General Tumour Biomarker and Contributes to the Development of Cutaneous Squamous Cell Carcinoma(2024/04/08)
作者:Ruiting Luo, Yingjian Huang, Ruimin Bai
期刊:ACTA DERMATO-VENEREOLOGICA
影响因子 :3.600
引用产品:CL-0015
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The Fgl2 interaction with Tyrobp promotes the proliferation of cutaneous squamous cell carcinoma by regulating ERK-dependent autophagy(2022/01/01)
作者:Mei Zeng, Qingxiang Li, Junzhao Chen
期刊:International Journal of Medical Sciences
影响因子 :3.600
引用产品:CL-0015
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Alkannin exerts antitumor properties in cutaneous squamous cell carcinoma by inducing apoptosis and shifting the M1/M2 polarization of tumor-associated macrophages by upregulating PTEN(2022/11/11)
作者:Zhong-Zhao Zhang, Chang-Hui Wen, Min Jia, Hong-Qiang Zhang, Shao-Qin Sun
期刊:KAOHSIUNG JOURNAL OF MEDICAL SCIENCES
影响因子 :3.300
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NR5A2 promotes malignancy progression and mediates the effect of cisplatin in cutaneous squamous cell carcinoma(2024/02/15)
作者:Ye Wang, Yaxuan Cao, Shanshan Tang
期刊:Immunity Inflammation and Disease
影响因子 :3.200
引用产品:CL-0015
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Depletion of RIPK4 parallels higher malignancy potential in cutaneous squamous cell carcinoma(2022/02/10)
作者:Jing Xu, Dongping Wu, Bicheng Zhang
期刊:PeerJ
影响因子 :2.700
引用产品:CL-0015
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Artesunate restrains the malignant progression of human cutaneous squamous cell carcinoma cells via the suppression of the PI3K/AKT pathway(2022/03/19)
作者:Xinyan Huang, Songzhao Zhang, Wenxi Wang
期刊:Tissue & Cell
DOI:10.1016/j.tice.2022.101789
影响因子 :2.600
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UBE2C promotes malignancy of cutaneous squamous cell carcinoma(2023/11/16)
作者:Ruiting Luo, Ruimin Bai, Jiaqi Guo
期刊:SKIN RESEARCH AND TECHNOLOGY
影响因子 :2.200
引用产品:CL-0015
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