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产品概述
名称 | Calu-3 (人肺腺癌细胞(胸水)) (STR鉴定正确) |
别称 | CaLu-3; CALU-3; Calu 3; Calu3; CALU3 |
种属 | 人 |
生长特性 | 贴壁细胞 |
细胞形态 | 上皮细胞样 |
冻存条件 | 冻存液:55% 基础培养基+40%FBS+5%DMSO 温度:液氮 |
培养方案A(默认) |
生长培养基:
培养条件:
气相:空气,95%;CO2,5%, 温度:37℃
|
推荐传代比例 | 1:3-1:4 |
推荐换液频率 | 2-3次/周 |
注意事项 | 该细胞较难消化,注意延长消化时间,消化到细胞收缩变圆,轻拍培养瓶侧边,细胞可以滑落方可终止消化。 |
背景描述 | Calu-3细胞是从一名25岁的白人男性肺腺癌患者的胸水中分离建系的;该患者曾使用过环磷酰胺、博来霉素、阿霉素进行治疗。Calu-3细胞接种至裸鼠可成瘤;Calu-3细胞亦可作转染宿主。 |
年龄(性别) | 男性;25岁 |
组织来源 | 肺;转移灶;胸水腺癌 |
细胞类型 | 肿瘤细胞 |
肿瘤类型 | 肺癌细胞 |
生物安全等级 | BSL-1 |
倍增时间 | ~35-45 hours |
致瘤性 | Yes, forms well differentiated grade I adenocarcinoma in nude mice. |
抗原表达情况 | Blood Type A; Rh+ |
基因表达情况 | Blood Type A; Rh+ |
保藏机构 | ATCC; HTB-55 BCRJ; 0264 |
STR鉴定
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STR位点信息
Amelogenin X CSF1PO 11,12 D2S1338 19 D3S1358 15,18 D5S818 11 D7S820 10,11 D8S1179 11,15 D13S317 12 D16S539 12,14 D18S51 14 ,17 D19S433 12,13 D21S11 28,30 FGA 25 PentaD 9,16 PentaE 5,21 TH01 6,9.3 TPOX 8 vWA 16,17 D6S1043 11 D12S391 21,22 D2S441 14 -
STR鉴定图
参考文献
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APOE interacts with ACE2 inhibiting SARS-CoV-2 cellular entry and inflammation in COVID-19 patients(2022/08/01)
作者:Hongsheng Zhang, Lin Shao, Zhihao Lin
期刊:Signal Transduction and Targeted Therapy
DOI:10.1038/s41392-022-01118-4
影响因子 :39.300
引用产品:CL-0054
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The HNF4α-BC200-FMR1 positive feedback loop promotes growth and metastasis in invasive mucinous lung adenocarcinoma(2021/01/01)
作者:Xiong Chen, Yujie Zhao, Daxuan Wang
期刊:Cancer Res
DOI:10.1158/0008-5472.CAN-21-0980
影响因子 :11.200
引用产品:CL-0054
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Downregulation of the Circadian Rhythm Regulator HLF Promotes Multiple-Organ Distant Metastases in Non-Small Cell Lung Cancer Through PPAR NF-κb Signaling(2020/07/10)
作者:Aibin Liu
期刊:Cancer Letters
DOI:10.1016/j.canlet.2020.04.007
影响因子 :9.700
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Targeting TYK2 alleviates Rab27A-induced malignant progression of non-small cell lung cancer via disrupting IFNα-TYK2-STAT-HSPA5 axis(2024/03/23)
作者:Yuanyuan Zeng, Jian Zhao, Zhengyan Wu
期刊:npj Precision Oncology
DOI:10.1038/s41698-024-00574-1
影响因子 :7.900
引用产品:CL-0054
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Activation of Hypoxia Inducible Factor-1 Alpha-Mediated DNA Methylation Enzymes (DNMT3a and TET2) Under Hypoxic Conditions Regulates S100A6 Transcription to Promote Lung Cancer Cell Growth and Metastasis(2024/03/18)
作者:Tengfei Wang, Genbao Zhu, Bo Wang
期刊:ANTIOXIDANTS & REDOX SIGNALING
影响因子 :6.600
引用产品:CL-0054
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Characteristics of replication and pathogenicity of SARS-CoV-2 Alpha and Delta isolates(2022/09/24)
作者:Xiaoli Feng, Dandan Yu, Mi Zhang
期刊:VIROLOGICA SINICA
DOI:10.1016/j.virs.2022.09.007
影响因子 :5.500
引用产品:CL-0054
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GPR37-centered ceRNA network contributes to metastatic potential in lung adenocarcinoma: Evidence from high-throughput sequencing(2023/11/16)
作者:Chuanhui Chen, Mengzhi Wan, Xiong Peng
期刊:Translational Oncology
DOI:10.1016/j.tranon.2023.101819
影响因子 :5.000
引用产品:CL-0054
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Synergistic inhibition effects of andrographolide and baicalin on coronavirus mechanisms by downregulation of ACE2 protein level(2024/02/21)
作者:Lina Wan, Yuchen Li, Wenhao Liao
期刊:Scientific Reports
DOI:10.1038/s41598-024-54722-5
影响因子 :4.600
引用产品:CL-0054
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Decreased Sphingosine Due to Down-Regulation of Acid Ceramidase Expression in Airway of Bronchiectasis Patients: A Potential Contributor to Pseudomonas aeruginosa Infection(2023/04/27)
作者:Qian Qi, Jiawei Xu, Yujiao Wang
期刊:Infection and Drug Resistance
影响因子 :3.900
引用产品:CL-0054
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Comprehensive Analysis of a Novel Immune-Related Gene Signature in Lung Adenocarcinoma(2022/10/19)
作者:Hongxiang Feng, Chaoyang Liang, Yuhui Shi
期刊:Journal of Clinical Medicine
影响因子 :3.900
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CircRNA ZNF609 Knockdown Represses the Development of Non-Small Cell Lung Cancer via miR-623/FOXM1 Axis(2022/12/21)
作者:Fanghan Wang, Xiangfeng Li, Xigao Jia
期刊:Cancer Management and Research
影响因子 :3.602
引用产品:CL-0054
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Hsa_circ_0080608 Attenuates Lung Cancer Progression by Functioning as a Competitive Endogenous RNA to Regulate the miR-661/ADRA1A Pathway(2023/10/11)
作者:Chengbo Ren, Ling Cui, Ruibiao Li
期刊:HORMONE AND METABOLIC RESEARCH
影响因子 :2.200
引用产品:CL-0054
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