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产品概述
| 名称 | HCT-15 (人结直肠腺癌细胞) (STR鉴定正确) |
| 别称 | HCT-15; HCT15 |
| 种属 | 人 |
| 生长特性 | 贴壁细胞 |
| 细胞形态 | 上皮细胞样 |
| 冻存条件 | 冻存液：55% 基础培养基+40%FBS+5%DMSO 温度：液氮 |
| 培养方案A（默认） |
培养条件:
气相：空气，95%；CO2，5%, 温度：37℃
|
| 推荐传代比例 | 1:3-1:4 |
| 推荐换液频率 | 2-3次/周 |
| 背景描述 | DNA指纹鉴定证据表明，HCT-15细胞和DLD-1细胞来源于同一个人；但同工酶及细胞染色体组型分析仍存疑问。HCT-15细胞呈CSAp阴性(CSAp-)；HCT-15角蛋白免疫过氧化物酶染色阳性。 |
| 年龄（性别） | 男性 |
| 组织来源 | 结直肠腺癌 |
| 细胞类型 | 肿瘤细胞 |
| 肿瘤类型 | 肠癌细胞 |
| 生物安全等级 | BSL-1 |
| 倍增时间 | ~20-25 hours |
| 致瘤性 | Yes, in nude mice (Tumors developed within 21 days at 100% frequency (5/5) in nude mice inoculated subcutaneously with 1×10^7 cells). |
| 基因表达情况 | carcinoembryonic antigen (CEA) 5.4 ng/10^6 cells/10 days. The cells are positive for keratin by immunoperoxidase staining. |
| 保藏机构 | ATCC; CCL-225 BCRC; 60539 DSMZ; ACC-357 ECACC; 91030712 |
STR鉴定
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STR位点信息
Amelogenin X,Y CSF1PO 12 D2S1338 17,25 D3S1358 17 D5S818 13 D7S820 10,12 D8S1179 15 D13S317 8,11 D16S539 12,13 D18S51 11,17 D19S433 14,16 D21S11 29,32.2 FGA 22 PentaD 9,14 PentaE 7,14 TH01 7,9.3 TPOX 8,11 vWA 18,19 D6S1043 11,13 D12S391 19,22 D2S441 13,15 -
STR鉴定图
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参考文献
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D3S-001, a KRAS G12C inhibitor with rapid target engagement kinetics, overcomes nucleotide cycling and demonstrates robust preclinical and clinical activities(2024/05/08)
作者：Jing Zhang, Sunmin Lim, Mira Yu
期刊：Cancer Discovery
DOI：10.1158/2159-8290.CD-24-0006
影响因子 ：28.200
引用产品：CL-0097
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Elevated expression of the rhythm gene NFIL3 promotes the progression of TNBC by activating NF-κB signaling through suppression of NFKBIA transcription(2022/02/18)
作者：Weiwei Yang , Jing Li, Minghui Zhang
期刊：Journal Of Experimental & Clinical Cancer Research
DOI：10.1186/s13046-022-02260-1
影响因子 ：11.300
引用产品：CL-0016 CL-0041 CL-0097 CL-0101 CL-0114 CL-0184 CL-0215 CL-0525
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Design, Synthesis, and Antitumor Activity Evaluation of Novel VISTA Small Molecule Inhibitors(2024/02/27)
作者：Chengliang Sun, Yuling He, Gefei Wang
期刊：JOURNAL OF MEDICINAL CHEMISTRY
DOI：10.1021/acs.jmedchem.3c02039
影响因子 ：7.300
引用产品：CL-0097
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LncRNA-ENST00000543604 exerts a tumor-promoting effect via miRNA 564/AEG-1 or ZNF326/EMT and predicts the prognosis of and chemotherapeutic effect in colorectal cancer.(2022/08/23)
作者：Weimin Wang, Zhen Zhou, Xiaojun Dai
期刊：Frontiers in Oncology
影响因子 ：5.738
引用产品：CL-0097
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CircFAM120B Blocks the Development of Colorectal Cancer by Activating TGF-Beta Receptor II Expression via Targeting miR-645(2021/07/26)
作者：You Yu, Xiao Lei
期刊：Frontiers in Cell and Developmental Biology
影响因子 ：5.500
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Discovery of Novel 4-Hydroxyquinazoline Derivatives: In Silico, In Vivo and In Vitro Studies Using Primary PARPi-Resistant Cell Lines(2024/03/21)
作者：Lijie Zhu, Binzhuo Liu, Feng Jin
期刊：MOLECULES
影响因子 ：4.600
引用产品：CL-0097
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Identification of KIF21B as a Biomarker for Colorectal Cancer and Associated with Poor Prognosis(2022/11/21)
作者：Shanshan Xu, Youran Li, Hua Huang, Xian Miao, Yunfei Gu
期刊：Journal of Oncology
影响因子 ：4.501
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LncRNA ENSG00000254615 Modulates Proliferation and 5-FU Resistance by Regulating p21 and Cyclin D1 in Colorectal Cancer(2021/5/1)
作者：Yang Fu, Runqing Huang, Jianxia Li
期刊：Cancer Investigation
DOI：10.1080/07357907.2021.1923727
影响因子 ：2.400
引用产品：CL-0097
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