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人胚肺成纤维细胞
Human Embryonic Lung Fibroblast Cells
货号:CP-H159
规格:5×10⁵Cells/T25培养瓶
价格: ¥6880
培养基货号: CM-H159
产品概述
人胚肺成纤维细胞
Cat NO.: CP-H159
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产品名称:人胚肺成纤维细胞
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组织来源:胚胎;肺组织
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产品规格:5×10⁵Cells/T25培养瓶
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细胞简介:
人胚肺成纤维细胞分离自胚肺组织;肺是机体的呼吸器官,位于胸腔,左右各一,覆盖于心之上。肺有分叶,左二右三,共五叶。肺经肺系(指气管、支气管等)与喉、鼻相连,故称喉为肺之门户,鼻为肺之外窍。成纤维细胞(Fibroblast)是疏松结缔组织的主要细胞成分,由胚胎时期的间充质细胞分化而来。成纤维细胞较大,轮廓清楚,多为突起的纺锤形或星形的扁平状结构,其细胞核呈规则的卵圆形,核仁大而明显。成纤维细胞功能活动旺盛,细胞质嗜弱碱性,具明显的蛋白质合成和分泌活动,在一定条件下,它可以实现跟纤维细胞的互相转化;成纤维细胞对不同程度的细胞变性、坏死和组织缺损的修复有着十分重要的作用。刚分离的胚肺成纤维细胞呈圆形、折光性良好,悬浮于培养基中。30min细胞贴壁,其中部分开始伸出伪足,表现为小的突起;6h后细胞基本贴壁完全,伸展成梭形,胞核清晰,分布较均匀,散在生长,不聚集成团;细胞生长迅速,5-7天即呈融合状态,细胞排列紧密,有的交叉重叠生长,平坦、胞体较大,细胞质透明,细胞核较大,呈椭圆形,颜色淡。细胞融合,并彼此连接成网状;细胞呈突起的纺锤形或星形的扁平分布。细胞呈突起的纺锤形或星形的扁平分布。该细胞在合成和分泌细胞因子、维持血管内外和凝血和纤溶的的动态平衡中起重要作用。
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方法简介:
普诺赛实验室分离的人胚肺成纤维细胞采用胰蛋白酶 - 胶原酶混合消化法结合差速贴壁法制备而来,细胞总量约为5×10⁵cells/瓶。
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质量检测:
普诺赛实验室分离的人胚肺成纤维细胞经Vimentin免疫荧光鉴定,纯度可达90%以上,且不含有HIV-1、HBV、HCV、支原体、细菌、酵母和真菌等。
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培养信息:
培养基 含FBS、生长添加剂、Penicillin、Streptomycin等 产品货号 CM-H159 换液频率 每2-3天换液一次 生长特性 贴壁 细胞形态 成纤维细胞样 传代特性 可传5代左右;3代以内状态最佳 消化液 0.25%胰蛋白酶 人胚肺成纤维细胞体外培养周期有限;建议使用普诺赛配套的专用生长培养基及正确的操作方法来培养,以此保证该细胞的最佳培养状态。
参考文献
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ROS-sensitive Crocin-loaded chitosan microspheres for lung targeting and attenuation of radiation-induced lung injury (2023-01-28)
作者:Lu Wang:1.State Key Laboratory of Radiation Medicine and Protection, School for Radiological and Interdisciplinary Sciences (RAD-X), Collaborative Innovation Center of Radiological Medicine of Jiangsu Higher Education Institutions, Soochow University, Suzhou 215123, China. ; Chang Liu:1.State Key Laboratory of Radiation Medicine and Protection, School for Radiological and Interdisciplinary Sciences (RAD-X), Collaborative Innovation Center of Radiological Medicine of Jiangsu Higher Education Institutions, Soochow University, Suzhou 215123, China. ; Weihong Lu:1.State Key Laboratory of Radiation Medicine and Protection, School for Radiological and Interdisciplinary Sciences (RAD-X), Collaborative Innovation Center of Radiological Medicine of Jiangsu Higher Education Institutions, Soochow University, Suzhou 215123, China. Electronic address: luweihong@suda.edu.cn. ; Longjiang Xu:1.Department of Pathology, The Second Affiliated Hospital of Soochow University, Suzhou 215000, China. Elect
期刊:ROS-sensitive Crocin-loaded chitosan microspheres for lung targeting and attenuation of radiation-induced lung injury
DOI:10.1016/j.carbpol.2023.120628
影响因子 :11.2
引用产品: BEAS-2B 细胞 , 人胚肺成纤维细胞 , 人脐静脉内皮细胞
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Integrated bioinformatics analysis for the identification of idiopathic pulmonary fibrosis–related genes and potential therapeutic drugs (2023-10-04)
作者:Zhenzhen Zhang:1.Academy of Chinese Medical Sciences, Henan University of Chinese Medicine, Zhengzhou, 450046, China. ; 2.Henan Key Laboratory of Chinese Medicine for Respiratory Disease, Collaborative Innovation Center for Chinese Medicine and Respiratory Diseases Co-Constructed By Henan Province and Education Ministry of P.R. China, Henan University of Chinese Medicine, Zhengzhou, 450046, China. ; Qingzhou Guan:1.Academy of Chinese Medical Sciences, Henan University of Chinese Medicine, Zhengzhou, 450046, China. a5410980@163.com. ; 2.Henan Key Laboratory of Chinese Medicine for Respiratory Disease, Collaborative Innovation Center for Chinese Medicine and Respiratory Diseases Co-Constructed By Henan Province and Education Ministry of P.R. China, Henan University of Chinese Medicine, Zhengzhou, 450046, China. a5410980@163.com. ; Yange Tian:1.Academy of Chinese Medical Sciences, Henan University of Chinese Medicine, Zhengzhou, 450046, China. ; 2.Henan Key Laboratory of Chinese Medicin
期刊:Integrated bioinformatics analysis for the identification of idiopathic pulmonary fibrosis–related genes and potential therapeutic drugs
DOI:10.1186/s12890-023-02678-z
影响因子 :3.1
FAQs
Q:原代上皮细胞是否可以用除鼠尾胶原外的其他的包被试剂包被
A:理论上各种包被试剂都是具有增加吸附性的效果,鼠尾胶原蛋白I型是一种常用的细胞外基质成分,能够模拟体内细胞生长的真实环境,特别适合于促进上皮细胞等需要良好贴壁的细胞类型贴附和生长。上皮细胞在体内外都需要特定的细胞-细胞和细胞-基质相互作用才能正常增殖和分化,鼠尾胶原能够提供这样的有利基质,帮助细胞更好地粘附和伸展,从而维持正常的细胞功能和形态。
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