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人骨髓间充质干细胞
Human Bone Marrow Mesenchymal Stem Cells
货号：CP-H166
规格：5×10⁵Cells/T25培养瓶
价格： ￥7380
培养基货号： CM-H166
产品概述
人骨髓间充质干细胞
Cat NO.: CP-H166
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产品名称：人骨髓间充质干细胞
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英文简称：BMSCs,MSCs
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组织来源：骨髓
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产品规格：5×105cells/T25细胞培养瓶
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细胞简介：
人骨髓间充质干细胞(BMSC)分离自骨髓；骨髓是机体的造血组织，位于身体的许多骨骼内。成年动物的骨髓分两种：红骨髓和黄骨髓。红骨髓能制造红细胞、血小板和各种白细胞。血小板有止血作用，白细胞能杀灭与抑制各种病原体，包括细菌、病毒等；某些淋巴细胞能制造抗体。因此，骨髓不但是造血器官，它还是重要的免疫器官。骨髓是存在于长骨(如肱骨、股骨)的骨髓腔和扁平骨(如髂骨)的稀松骨质间的网眼中，是一种海绵状的组织，能产生血细胞的骨髓略呈红色，称为红骨髓。出生时，红骨髓充满全身骨髓腔，随着年龄增大，脂肪细胞增多，相当部分红骨髓被黄骨髓取代，最后几乎只有扁平骨骨髓腔中有红骨髓。骨髓基质系统内存在的骨髓间充质干细胞是一种除造血干细胞以外的、具有高度自我更新能力和多向分化潜能的干细胞，可以向骨、软骨、肌组织、皮肤、脂肪、神经等多种组织分化，因此可以作为组织工程中的种子细胞。在骨髓中，BMSC占骨髓有核细胞总数的0.001%-0.1%，含量极低。骨髓间充质干细胞的体外培养条件要求较高，在培养过程中，受贴壁时间、种植密度、血清含量、培养温度和培养基pH值等条件的影响。
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方法简介：
普诺赛实验室分离的人骨髓间充质干细胞采用冲洗骨髓、密度梯度离心、差速贴壁法结合培养基筛选制备而来，细胞总量约为5×10⁵cells/瓶。
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质量检测：
普诺赛实验室分离的人骨髓间充质干细胞经CD29或CD90免疫荧光鉴定，纯度可达90%以上，且不含有HIV-1、HBV、HCV、支原体、细菌、酵母和真菌等。
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培养信息：
包被条件 培养基 含FBS、生长添加剂、Penicillin、Streptomycin等 产品货号 CM-H166 换液频率 每2-3天换液一次 生长特性 贴壁 细胞形态 成纤维细胞样 传代特性 可传3-5代左右；3代以内状态最佳 消化液 0.25%胰蛋白酶 培养条件 气相：空气，95%；CO2，5% 人骨髓间充质干细胞体外培养周期有限；建议使用普诺赛配套的专用生长培养基及正确的操作方法来培养，以此保证该细胞的最佳培养状态。
参考文献
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Spontaneous immunomodulation and regulation of angiogenesis and osteogenesis by Sr/Cu-borosilicate glass (BSG) bone cement to repair critical bone defects(2022/11/09)
作者：Shuaijie Li, Liyan Zhang, Chunyu Liu
期刊：Bioactive Materials
DOI：10.1016/j.bioactmat.2022.10.021
影响因子 ：16.874
引用产品：CP-H166
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An injectable self-adaptive polymer as a drug carrier for the treatment of nontraumatic early-stage osteonecrosis of the femoral head(2022/03/12)
作者：Ning Kong, Hang Yang, Run Tian
期刊：Bone Research
DOI：10.1038/s41413-022-00196-y
影响因子 ：12.700
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Cell membrane vesicles derived from hBMSCs and hUVECs enhance bone regeneration(2024/04/09)
作者：Dandan Wang, Yaru Guo, Xuehui Zhang
期刊：Bone Research
DOI：10.1038/s41413-024-00325-9
影响因子 ：12.700
引用产品：CP-H166
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pH-triggered chitosan-sodium caseinate nanocarriers with charge-switching property: Characterization and applications in dental care(2024/02/23)
作者：Mingxia Wang, Yaqian Li, Yuxuan Zhao
期刊：FOOD HYDROCOLLOIDS
DOI：10.1016/j.foodhyd.2024.109919
影响因子 ：10.700
引用产品：CP-H166
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HWJMSC-EVs promote cartilage regeneration and repair via the ITGB1/TGF-β/Smad2/3 axis mediated by microfractures(2024/04/12)
作者：Zhian Chen, Tianhua Zhou, Huan Luo
期刊：JOURNAL OF NANOBIOTECHNOLOGY
DOI：10.1186/s12951-024-02451-2
影响因子 ：10.200
引用产品：CP-H166
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Bone-targeting exosome nanoparticles activate Keap1 / Nrf2 / GPX4 signaling pathway to induce ferroptosis in osteosarcoma cells(2023/09/30)
作者：Wenkai Chen, Zongguang Li, Naichun Yu
期刊：JOURNAL OF NANOBIOTECHNOLOGY
DOI：10.1186/s12951-023-02129-1
影响因子 ：10.200
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Activation of Dynamin-Related Protein 1 and Induction of Mitochondrial Apoptosis by Exosome-Rifampicin Nanoparticles Exerts Anti-Osteosarcoma Effect(2022/11/17)
作者：Wenkai Chen, Wenping Lin, Naichun Yu, Linlin Zhang, Zuoxing Wu, Yongjie Chen, Zongguang Li, Fengqing Gong, Na Li, Xiaohui Chen, Xu He, Yue Wu, Xiangchen Zeng, Yuting Yueh, Ren Xu, Guangrong Ji
期刊：International Journal of Nanomedicine
影响因子 ：8.000
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Bioinformatic analysis of related immune cell infiltration and key genes in the progression of osteonecrosis of the femoral head(2024/01/11)
作者：Xudong Duan, Fangze Xing, Jiewen Zhang
期刊：Frontiers in Immunology
DOI：10.3389/fimmu.2023.1340446
影响因子 ：7.300
引用产品：CP-H166
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Human bone marrow mesenchymal stem cell-derived extracellular vesicles reduce inflammation and pyroptosis in acute kidney injury via miR-223-3p/HDAC2/SNRK(2023/01/14)
作者：Zhijuan Xie, Jun Tang, Zhong Chen
期刊：Inflammation Research
DOI：10.1007/s00011-022-01653-4
影响因子 ：6.700
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CircRNA AFF4 induced by KDM1A promotes osteogenic differentiation through FNDC5/Irisin pathway(2022/11/18)
作者：Liu Ansong, Chen Yong, Zhong Da, Wang Chenggong, Yu Mi, Liu Chao, Yang Zhijun, Chen Wenkang, Yin Ke
期刊：MOLECULAR MEDICINE
DOI：10.1186/s10020-022-00557-7
影响因子 ：5.700
引用产品：CP-H166
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Osteogenesis-Related Long Noncoding RNA GAS5 as a Novel Biomarker for Osteonecrosis of Femoral Head.(2022/03/22)
作者：Guanzhi Liu, Sen Luo, Yutian Lei
期刊：Frontiers in Cell and Developmental Biology
影响因子 ：5.500
引用产品：CP-H166
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Inhibitory role of bone marrow mesenchymal stem cells-derived exosome in non-small-cell lung cancer: microRNA-30b-5p, EZH2 and PI3K/AKT pathway(2023/09/12)
作者：Tong Wu, Qi Tian, Ruiji Liu
期刊：JOURNAL OF CELLULAR AND MOLECULAR MEDICINE
影响因子 ：5.300
引用产品：164210 CL-0005 CL-0016 CL-0165 CM-H166 CP-H166 PB180120 PM150210 PM150910
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Trifluoperazine activates AMPK / mTOR / ULK1 signaling pathway to induce mitophagy in osteosarcoma cells(2024/02/13)
作者：Wenhui Shen, Xianhui Zeng, Xiangchen Zeng
期刊：CHEMICO-BIOLOGICAL INTERACTIONS
影响因子 ：5.100
引用产品：CP-H166
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Injectable EC-BMSC hydrogel with prolonged VEGF action for enhanced angiogenesis(2023/06/21)
作者：Shuqin Chen, Bing Han, Yanran Zhao
期刊：POLYMER TESTING
DOI：10.1016/j.polymertesting.2023.108109
影响因子 ：5.100
引用产品：CP-H166
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Exosomal microRNA-618 derived from mesenchymal stem cells attenuate the progression of hepatic fibrosis by targeting Smad4(2022/02/24)
作者：Chao Sun, Cuicui Shi, Xiaoyan Duan
期刊：Bioengineered
DOI：10.1080/21655979.2021.2023799
影响因子 ：4.900
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MSC-Derived Exosomes Ameliorate Intervertebral Disc Degeneration By Regulating the Keap1/Nrf2 Axis(2023/08/01)
作者：Guangyu Xu, Xiao Lu, Siyang Liu
期刊：Stem Cell Reviews and Reports
DOI：10.1007/s12015-023-10570-w
影响因子 ：4.800
引用产品：CP-H166
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Conductive material Gel@Ti3C2/MT regulates the PI3K/AKT/HIF-1α/TIMP1 pathway affecting MT1 for inhibiting osteosarcoma(2024/04/16)
作者：Weilin Zhang, Jinguo Liang, Dingbin Li
期刊：CHEMICAL ENGINEERING SCIENCE
影响因子 ：4.700
引用产品：CP-H166
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Incorporating GSE4 peptide in PEG/hyaluronic acid hydrogels to promote the alveolar epithelial differentiation of mesenchymal stem cells(2023/03/09)
作者：Xiaoqiong Wang, Leisha Cui, Jing Hong
期刊：POLYMER
DOI：10.1016/j.polymer.2023.125861
影响因子 ：4.600
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Mesenchymal stem cell-derived exosomal miR-548x-3p inhibits pyroptosis of vascular endothelial cells through HMGB1 in heat stroke(2023/09/25)
作者：Yanfang Pei, Wenfeng Ma, Huifang Wang
期刊：GENOMICS
DOI：10.1016/j.ygeno.2023.110719
影响因子 ：4.400
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Extracellular vesicles derived from hypoxia-preconditioned bone marrow mesenchymal stem cells ameliorate lower limb ischemia by delivering miR-34c(2023/02/02)
作者：Xitao Peng, Junhua Liu, Lijun Ren
期刊：MOLECULAR AND CELLULAR BIOCHEMISTRY
DOI：10.1007/s11010-023-04666-7
影响因子 ：4.300
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Long non-coding RNA DANCR modulates osteogenic differentiation by regulating the miR-1301-3p/PROX1 axis(2021/2/24)
作者：Wei Weng, Shengdi Di, Shitong Xing
期刊：Molecular And Cellular Biochemistry
DOI：10.1007/s11010-021-04074-9
影响因子 ：4.300
引用产品：CP-H166
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Analysis of the potential biological mechanisms of Danyu Gukang Pill against osteonecrosis of the femoral head based on network pharmacology(2023/01/31)
作者：Yongchang Guo, Wenxi Li, Yuju Cao
期刊：BMC Complementary Medicine and Therapies
DOI：10.1186/s12906-023-03843-x
影响因子 ：3.900
引用产品：CP-H166
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Exosomes derived from TSG-6 modified mesenchymal stromal cells attenuate scar formation during wound healing(2020/08/12)
作者：Lan Jiang, Yanguo Zhang, Tao Liu
期刊：Biochimie
DOI：10.1016/j.biochi.2020.08.003
影响因子 ：3.900
引用产品：CP-H166
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GelMA/κ-carrageenan double-network hydrogels with superior mechanics and biocompatibility(2023/01/06)
作者：Xueqi Gan, Chen Li, Jiyu Sun
期刊：RSC Advances
影响因子 ：3.900
引用产品：CP-H166
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Mesenchymal stem cell exosomes inhibit nucleus pulposus cell apoptosis via the miR-125b-5p/TRAF6/NF-κB pathway axis: Exosomes attenuate disc degeneration through the miR-125b/TRAF6/NF-κB axis(2023/11/15)
作者：Yang Duan, Cheng Yu, Wenhao Kuang
期刊：ACTA BIOCHIMICA ET BIOPHYSICA SINICA
影响因子 ：3.700
引用产品：CP-H166
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Hypoxia pretreatment improves the therapeutic potential of bone marrow mesenchymal stem cells in hindlimb ischemia via upregulation of NRG-1(2022/01/29)
作者：Xitao Peng, Bing Liang, Haisheng Wang
期刊：Cell And Tissue Research
DOI：10.1007/s00441-021-03562-0
影响因子 ：3.600
引用产品：CP-H166
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Mesenchymal stromal cells can repair radiation-induced pulmonary fibrosis via a DKK-1-mediated Wnt/β-catenin pathway(2021/01/26)
作者：Lihong Shao, Yuyu Zhang
期刊：Cell And Tissue Research
DOI：10.1007/s00441-020-03325-3
影响因子 ：3.600
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Mesenchymal stem cell exosomal tsRNA-21109 alleviate systemic lupus erythematosus by inhibiting macrophage M1 polarization(2021/08/28)
作者：Rui Dou, Xiulei Zhang, Xiangdong Xu
期刊：Molecular Immunology
DOI：10.1016/j.molimm.2021.08.015
影响因子 ：3.600
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Mesenchymal stem cells-derived exosomes ameliorate high glucose and lipopolysaccharide-induced HPMECs injury through the Nrf2/HO-1 pathway(2023/12/31)
作者：Hongyan Sun, Shitao Lu, Gaowei Qu
期刊：AUTOIMMUNITY
DOI：10.1080/08916934.2023.2290357
影响因子 ：3.500
引用产品：CP-H166
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MEG3 shuttled by exosomes released from human bone marrow mesenchymal stem cells promotes TP53 stability to regulate MCM5 transcription in keloid fibroblasts(2024/04/30)
作者：Feibin Zhu, Yuanjian Ye, Ying Shao
期刊：JOURNAL OF GENE MEDICINE
DOI：10.1002/jgm.3688
影响因子 ：3.500
引用产品：CP-H166
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Omaveloxolone ameliorates glucocorticoid-induced osteonecrosis of the femoral head by promoting osteogenesis and angiogenesis(2024/05/31)
作者：Changtao Wan, Chuancai Liang, Hao Peng
期刊：BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
DOI：10.1016/j.bbrc.2024.150188
影响因子 ：3.100
引用产品：CP-H166
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High glucose levels delay the senescence of stem cells from human exfoliated deciduous teeth by suppressing autophagy(2023/11/17)
作者：Jinjin Lu, Lixin Zhang, Ningxin Zhu
期刊：ARCHIVES OF ORAL BIOLOGY
DOI：10.1016/j.archoralbio.2023.105851
影响因子 ：3.000
引用产品：CP-H166
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Notoginsenoside R1 promotes osteogenic differentiation of human bone marrow mesenchymal stem cells via ERα/GSK-3β/β-catenin signalling pathway(2023/10/30)
作者：Wei Lu, Yuanxin Shi, Minglei Qian
期刊：INTERNATIONAL JOURNAL OF EXPERIMENTAL PATHOLOGY
影响因子 ：3.000
引用产品：CP-H166
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Human bone marrow mesenchymal stem cells-derived exosomes protect against nerve injury via regulating immune microenvironment in neonatal hypoxic-ischemic brain damage model(2022/01/10)
作者：Jiaping Shu, Li Jiang, Meiqiu Wang
期刊：Immunobiology
DOI：10.1016/j.imbio.2022.152178
影响因子 ：2.800
引用产品：CP-H166
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Cimigenol depresses acute myeloid leukemia cells protected by breaking bone marrow stromal cells via CXCR4/SDF‑1α(2022/12/30)
作者：Bangyun Ma, Huibo Dai, Xingbin Dai
期刊：Experimental and Therapeutic Medicine
影响因子 ：2.700
引用产品：CP-H166
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Long non-coding RNA taurine upregulated gene 1 is downregulated in osteoporosis and influences the osteogenic differentiation of bone marrow mesenchymal stem cells(2021/4/20)
作者：Zhaowei Teng, Yun Zhu, Qinggang Hao
期刊：PeerJ
影响因子 ：2.700
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LINC01234 Sponging of the miR-513a-5p/AOX1 Axis is Upregulated in Osteoporosis and Regulates Osteogenic Differentiation of Bone Marrow Mesenchymal Stem Cells(2023/03/24)
作者：Zhiyuan Yan, Qiansong He
期刊：MOLECULAR BIOTECHNOLOGY
DOI：10.1007/s12033-023-00712-3
影响因子 ：2.600
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MiR-145 inhibits the differentiation and proliferation of bone marrow stromal mesenchymal stem cells by GABARAPL1 in steroid-induced femoral head necrosis(2022/11/26)
作者：Xu Pengfei, Chang Jun, Ma Guangwen, Liao Faxue, Xu Tangbing, Wu Yunfeng, Yin Zongsheng
期刊：BMC MUSCULOSKELETAL DISORDERS
DOI：10.1186/s12891-022-05928-z
影响因子 ：2.300
引用产品：CP-H166
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YTHDF2-Mediated m6A methylation inhibition by miR27a as a protective mechanism against hormonal osteonecrosis in BMSCs(2024/05/06)
作者：Tianyi Yuan, Hongjiang Liu, Abudoukadier Maimaitiyibubaji
期刊：BMC MUSCULOSKELETAL DISORDERS
DOI：10.1186/s12891-024-07481-3
影响因子 ：2.300
引用产品：CP-H166
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Ubiquitination of ASCL1 mediates CD47 transcriptional activation of the AKT signaling pathway, and glycolysis promotes osteogenic differentiation of hBMSCs(2023/10/02)
作者：Jimei Zhang, Ling Zhu, Jianping Zhou
期刊：IN VITRO CELLULAR & DEVELOPMENTAL BIOLOGY-ANIMAL
DOI：10.1007/s11626-023-00811-0
影响因子 ：2.100
引用产品：CP-H166
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LncRNA TUG regulates osteogenic differentiation of bone marrow mesenchymal stem cells via miRNA-204/SIRT 1(2022/09/01)
作者：Xiao Ouyang, Yunzhi Ding, Li Yu
期刊：JOURNAL OF MUSCULOSKELETAL & NEURONAL INTERACTIONS
DOI：PMID:36046997
影响因子 ：1.900
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MicroRNA-4461 derived from bone marrow mesenchymal stem cell exosomes inhibits tumorigenesis by downregulating COPB2 expression in colorectal cancer(2019/10/21)
作者：Huili Chen, Jiujiang Li, Fei Jiang
期刊：Bioscience Biotechnology And Biochemistry
DOI：10.1080/09168451.2019.1677452
影响因子 ：1.600
引用产品：CP-H166
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