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产品概述
产品信息
| 别名 | Urogastrone, URG |
| 物种 | Human |
| 表达宿主 | E.coli |
| 序列信息 | MNSDSECPLSHDGYCLHDGVCMYIEALDKYACNCVVGYIGERCQYRDLKWWELR with polyhistidine tag at the C-terminus |
| 检索号 | P01133.2 |
| 分子量 | 7.16 kDa |
| 表观分子量 | 9-11 kDa |
| 标签 | His-tag at the C-terminus |
| 生物活性 | Measure by its ability to induce 3T3 cells proliferation. The ED50 for this effect is 0.05-0.12 ng/mL. The specific activity of recombinant human EGF is approximately >1.4 x 10^6 IU/mg. |
产品特性
| 纯度 | >95% as determined by SDS-PAGE. Ni-NTA chromatography. |
| 内毒素 | <0.1 EU per 1 μg of the protein by the LAL method. |
| 保存 | Lyophilized protein should be stored at -5~-20°C for 1 year. Upon reconstitution, store at 2-8°C for up to 1 week. Further dilute in a buffer containing a carrier protein or stabilizer (e.g.0.1% BSA,10%FBS,5%HSA or 5% trehalose solution), protein aliquots should be stored at -5~-20°C or -80°C for 3-6 months. |
| 运输 | Ambient temperature or ice pack. |
| 制剂 | The protein was lyophilized from a 0.2 µm filtered solution containing 1X PBS, pH 8.0. |
| 复融 | It is recommended to reconstitute the lyophilized protein in sterile water to a concentration not less than 100 μg/mL.Do Not Vortex! Vigorous shaking may impair the biological activity of the protein. |
背景介绍
Epidermal growth factor (EGF) stimulates cell growth and differentiation by binding to its receptor, EGFR. Human EGF is a 6-kDa protein with 53 amino acid residues and three intramolecular disulfide bonds. EGF is present in various body fluids, including blood, milk, urine, saliva, seminal fluid, pancreatic juice, cerebrospinal fluid, and amniotic fluid. Biological activities ascribed to EGF include epithelial development, angiogenesis, inhibition of gastric acid secretion, fibroblast proliferation, and colony formation of epidermal cells in culture.
参考文献
文献列表
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Activating SIRT3 in peritoneal mesothelial cells alleviates postsurgical peritoneal adhesion formation by decreasing oxidative stress and inhibiting the NLRP3 inflammasome(2022/09/13)
作者：Tianli Shen, Yunhua Wu, Xingjie Wang
期刊：EXPERIMENTAL AND MOLECULAR MEDICINE
DOI：10.1038/s12276-022-00848-3
影响因子 ：12.800
-
Tumor-suppressive E3 ubiquitin ligase CHIP inhibits the PBK/ERK axis to repress stem cell properties and radioresistance in non-small cell lung cancer(2022/11/27)
作者：Tan, Bo, Zhang, Jingwei, Wang, Wen, Ma, Haibo, Yang, Yuanyuan
期刊：APOPTOSIS
DOI：10.1007/s10495-022-01789-y
影响因子 ：7.200
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