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产品概述
| 名称 | J774A.1 (小鼠单核巨噬细胞) (STR鉴定正确) |
| 别称 | J-774A.1; J774.A1; J774 A1; J774A.1; J 774A.1; J774 A.1 |
| 种属 | 小鼠 |
| 生长特性 | 贴壁细胞，不用胰酶消化 |
| 细胞形态 | 单核细胞、巨噬细胞样 |
| 冻存条件 | 冻存液：55% 基础培养基+40%FBS+5%DMSO 温度：液氮 |
| 培养方案A（默认） |
培养条件:
气相：空气，95%；CO2，5%, 温度：37℃
|
| 推荐传代比例 | 1:3-1:4 |
| 推荐换液频率 | 2-3次/周 |
| 注意事项 | 1.该细胞不建议使用胰酶消化，胰酶会刺激分化；2.超过3天不传代细胞容易分化；3.培养时，存在少量分化细胞，属于正常现象。 |
| 背景描述 | J774A.1细胞来源于BABL/cN小鼠，有抗体依赖的吞噬作用，生长受硫酸葡聚糖、PPD和LPS的抑制；J774A.1细胞可产生IL-1β和大量的溶菌酶。 |
| 年龄（性别） | 雌性；成年 |
| 组织来源 | 巨噬细胞 |
| 细胞类型 | 肿瘤细胞 |
| 肿瘤类型 | 肉瘤细胞 |
| 生物安全等级 | BSL-1 |
| 倍增时间 | ~17 hours |
| 受体表达情况 | Complement (C3), expressed;Fc receptor, IgG, high affinity I (Fcgr1), expressed |
| 保藏机构 | ATCC; TIB-67 DSMZ; ACC-170 ECACC; 91051511 |
STR鉴定
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STR位点信息
18-3 18 6-7 12 5-5 14 X-1 25 1-2 17,18 7-1 24.2,25.2 8-1 13,14 1-1 16,17 19-2 14,15 15-3 22.3 12-1 16 6-4 18 4-2 21.3 3-2 14 2-1 16,17 13-1 15.2,16.2
11-2 16,17 17-2 15,16,17 -
STR鉴定图
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参考文献
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An Engineered Bionic Nanoparticle Sponge as a Cytokine Trap and Reactive Oxygen Species Scavenger to Relieve Disc Degeneration and Discogenic Pain(2024/01/18)
作者：Wenbo Yang, Kanglu Li, Qing Pan
期刊：ACS Nano
影响因子 ：17.100
引用产品：CL-0370
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Inhalable vaccine of bacterial culture supernatant extract mediates protection against fatal pulmonary anthrax(2023/03/31)
作者：Lina Zhai, Yue Zhao, Xiaolin Song
期刊：Emerging Microbes & Infections
DOI：10.1080/22221751.2023.2191741
影响因子 ：13.200
引用产品：CL-0370
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Acute exposure to polystyrene nanoparticles promotes liver injury by inducing mitochondrial ROS-dependent necroptosis and augmenting macrophage-hepatocyte crosstalk(2024/04/12)
作者：Junjie Fan, Li Liu, Yongling Lu
期刊：Particle and Fibre Toxicology
DOI：10.1186/s12989-024-00578-6
影响因子 ：10.000
引用产品：CL-0370
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Cell-Penetrating Peptide-Functionalized Persistent Luminescence Nanoparticles for Tracking J774A.1 Macrophages Homing to Inflamed Tissues(2019/05/13)
作者：Lijian Chen, Xu Zhao, Xiuping Yan
期刊：ACS Applied Materials & Interfaces
影响因子 ：9.500
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The mechanism of adipose mesenchymal stem cells to stabilize the immune microenvironment of pelvic floor injury by regulating pyroptosis and promoting tissue repair(2023/12/18)
作者：Xiaotong Wu, Fengshi Zhang, Xiaolin Mao
期刊：Materials Today Bio
DOI：10.1016/j.mtbio.2023.100910
影响因子 ：8.200
引用产品：CL-0370
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Chalcone derivatives ameliorate lipopolysaccharide-induced acute lung injury and inflammation by targeting MD2(2021/09/03)
作者：Yali Zhang, Wenxin Zhang, Jueqian Yan
期刊：Acta Pharmacologica Sinica
DOI：10.1038/s41401-021-00764-8
影响因子 ：8.200
引用产品：CL-0370
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Repairing gastric ulcer with hyaluronic acid/extracellular matrix composite through promoting M2-type polarization of macrophages(2023/06/24)
作者：Renhao Ni, Yang Luo, Lingjing Jiang
期刊：INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
DOI：10.1016/j.ijbiomac.2023.125556
影响因子 ：8.200
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Mitigation of Sepsis-Induced Acute Lung Injury by BMSC-Derived Exosomal miR-125b-5p Through STAT3-Mediated Suppression of Macrophage Pyroptosis(2023/11/28)
作者：Yiming Tao, Xinxin Xu, Bin Yang
期刊：International Journal of Nanomedicine
影响因子 ：8.000
引用产品：CL-0370
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Xijiao Dihuang Decoction combined with Yinqiao Powder promotes autophagy-dependent ROS decrease to inhibit ROS/NLRP3/pyroptosis regulation axis in influenza virus infection(2024/02/10)
作者：Di Deng, Mengfan Zhao, Huanwei Liu
期刊：PHYTOMEDICINE
DOI：10.1016/j.phymed.2024.155446
影响因子 ：7.900
引用产品：CL-0370
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Precise Detection on Cell–Cell Fusion by a Facile Molecular Beacon-Based Method(2022/12/01)
作者：Chang Xu, Xiaohe Ren, Di Han
期刊：Analytical Chemistry
DOI：10.1021/acs.analchem.2c04852
影响因子 ：7.400
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M1 polarization enhances the antitumor activity of chimeric antigen receptor macrophages in solid tumors(2023/03/28)
作者：Yi Huo, Han Zhang, Longqi Sa
期刊：Journal of Translational Medicine
DOI：10.1186/s12967-023-04061-2
影响因子 ：7.400
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Salmonella spvC Gene Inhibits Autophagy of Host Cells and Suppresses NLRP3 as Well as NLRC4(2021/07/14)
作者：Liting Zhou, Yuanyuan Li, Song Gao
期刊：Frontiers in Immunology
影响因子 ：7.300
引用产品：CL-0370
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Silica-induced macrophage pyroptosis propels pulmonary fibrosis through coordinated activation of relaxin and osteoclast differentiation signaling to reprogram fibroblasts(2024/02/19)
作者：Jiaqi Tian, Dandan Song, Yanjie Peng
期刊：ECOTOXICOLOGY AND ENVIRONMENTAL SAFETY
DOI：10.1016/j.ecoenv.2024.116106
影响因子 ：6.800
引用产品：CL-0370
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Salmonella spvC gene suppresses macrophage/neutrophil antibacterial defense mediated by gasdermin D(2023/12/23)
作者：Liting Zhou, Yuanyuan Li, Jiayi You
期刊：INFLAMMATION RESEARCH
DOI：10.1007/s00011-023-01818-9
影响因子 ：6.700
引用产品：CL-0370
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Discovery of novel osthole derivatives exerting anti-inflammatory effect on DSS-induced ulcerative colitis and LPS-induced acute lung injury in mice(2024/02/22)
作者：Ying Zhou, Zhiteng Du, Qianqian Wu
期刊：EUROPEAN JOURNAL OF MEDICINAL CHEMISTRY
DOI：10.1016/j.ejmech.2024.116252
影响因子 ：6.700
引用产品：CL-0370
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Discovery of 4-oxo-N-phenyl-1,4-dihydroquinoline-3-carboxamide derivatives as novel anti-inflammatory agents for the treatment of acute lung injury and sepsis(2023/01/23)
作者：Jun Yang, Minxiu Wang, Yulan Xu
期刊：EUROPEAN JOURNAL OF MEDICINAL CHEMISTRY
DOI：10.1016/j.ejmech.2023.115144
影响因子 ：6.700
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Immunoactivity of a hybrid membrane biosurface on nanoparticles: enhancing interaction with dendritic cells to augment anti-tumor immune responses(2023/12/29)
作者：Luying Yu, Ao Zhou, Jingyan Jia
期刊：Biomaterials Science
影响因子 ：6.600
引用产品：CL-0370
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1,25(OH)2D3 Promotes Macrophage Efferocytosis Partly by Upregulating ASAP2 Transcription via the VDR-Bound Enhancer Region and ASAP2 May Affect Antiviral Immunity(2022/11/21)
作者：Hui Shi, Jiangling Duan, Jiayu Wang, Haohao Li, Zhiheng Wu, Shuaideng Wang, Xueyan Wu, Ming Lu
期刊：Nutrients
影响因子 ：5.900
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Inhibition of STAT3 phosphorylation by colchicine regulates NLRP3 activation to alleviate sepsis-induced acute lung injury(2023/04/28)
作者：Yuanshui Liu, Hang Yang, Fei Zhu
期刊：INFLAMMOPHARMACOLOGY
DOI：10.1007/s10787-023-01199-9
影响因子 ：5.800
引用产品：CL-0370
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Periplaneta americana extract alleviates steatohepatitis in a mouse model by modulating HMGB1-mediated inflammatory response(2022/10/03)
作者：Yang Xiao, Chongqing Gao, Junru Wu
期刊：Frontiers in Pharmacology
影响因子 ：5.600
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Selective Expression of a SNARE-Cleaving Protease in Peripheral Sensory Neurons Attenuates Pain-Related Gene Transcription and Neuropeptide Release(2021/8/17)
作者：Wanzhi Wang, Miaomiao Kong, Yu Dou
期刊：International Journal Of Molecular Sciences
影响因子 ：5.600
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Exosomes derived from vMIP-II-Lamp2b gene-modified M2 cells provide neuroprotection by targeting the injured spinal cord, inhibiting chemokine signals and modulating microglia/macrophage polarization in mice(2024/04/19)
作者：Guiqiang Fu, Yangyang Wang, Yaomei Xu
期刊：EXPERIMENTAL NEUROLOGY
DOI：10.1016/j.expneurol.2024.114784
影响因子 ：5.300
引用产品：CL-0370
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Salmonella spvC Gene Inhibits Pyroptosis and Intestinal Inflammation to Aggravate Systemic Infection in Mice(2020/12/15)
作者：Lingli Zuo, Liting Zhou, Chaoyi Wu
期刊：Frontiers in Microbiology
影响因子 ：5.200
引用产品：CL-0370
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Discovery of pterostilbene analogs as novel NLRP3 inflammasome inhibitors for potential treatment of DSS-induced colitis in mice(2023/02/18)
作者：Banfeng Ruan, Minghui Rong, Zibei Ming
期刊：BIOORGANIC CHEMISTRY
DOI：10.1016/j.bioorg.2023.106429
影响因子 ：5.100
引用产品：CL-0370
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The Extract of Scutellaria baicalensis Attenuates the Pattern Recognition Receptor Pathway Activation Induced by Influenza A Virus in Macrophages(2023/07/08)
作者：Mingrui Yang, Luyao Ma, Rina Su
期刊：Viruses-Basel
影响因子 ：4.700
引用产品：CL-0370
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Hunteriasines A – D, tryptamine-derived alkaloids from Hunteria umbellata(2023/06/15)
作者：Daijing Wei, Yun Yang, Ruiying Xi
期刊：PHYTOCHEMISTRY
DOI：10.1016/j.phytochem.2023.113752
影响因子 ：3.800
引用产品：CL-0370
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TWEAK promotes inflammatory response in liver fibrosis(2023/07/28)
作者：Yun Kong, Yi Yang, Shasha Wu
期刊：JOURNAL OF BIOCHEMICAL AND MOLECULAR TOXICOLOGY
影响因子 ：3.600
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Bif‑1 inhibits activation of inflammasome through autophagy regulatory mechanism(2024/03/01)
作者：Yuehui Zhang, Wenhui Su, Yaoyun Niu
期刊：Molecular Medicine Reports
影响因子 ：3.400
引用产品：CL-0370
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产品资料
识别码示意图
![Sp2/0-Ag14[SP2/0] (小鼠骨髓瘤细胞) (STR鉴定正确)](/Public/upfile/pic/xbx/CL-0445-1.jpg)
