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![293 [HEK-293] (人胚肾细胞) (STR鉴定正确)](/Public/upfile/pic/xbx/CL-0001-1-1.jpg)
![293 [HEK-293] (人胚肾细胞) (STR鉴定正确)](/Public/upfile/pic/xbx/CL-0001-1-2.jpg)
![293 [HEK-293] (人胚肾细胞) (STR鉴定正确)](/Public/upfile/pic/xbx/CL-0001-1-3.jpg)
![293 [HEK-293] (人胚肾细胞) (STR鉴定正确)](/Public/upfile/pic/xbx/CL-0001-1-4.jpg)
![293 [HEK-293] (人胚肾细胞) (STR鉴定正确)](/Public/upfile/pic/xbx/CL-0001-1-5.jpg)
![293 [HEK-293] (人胚肾细胞) (STR鉴定正确)](/Public/upfile/pic/xbx/CL-0001-1-6.jpg)
![293 [HEK-293] (人胚肾细胞) (STR鉴定正确)](/Public/upfile/pic/product/cell-line-new.jpg)
产品概述
| 名称 | 293 [HEK-293] (人胚肾细胞) (STR鉴定正确) |
| 别称 | Hek293; HEK-293; HEK 293; HEK:293; 293; 293 HEK; Human Embryonic Kidney 293 |
| 种属 | 人 |
| 生长特性 | 贴壁细胞 |
| 细胞形态 | 上皮细胞样 |
| 冻存条件 | 冻存液：55% 基础培养基+40%FBS+5%DMSO 温度：液氮 |
| 培养方案A（默认） |
生长培养基:
培养条件:
气相：空气，95%；CO2，5%, 温度：37℃
|
| 推荐传代比例 | 1:3-1:4 |
| 推荐换液频率 | 2-3次/周 |
| 注意事项 | 该细胞贴壁松散，操作时请尽量轻柔；换液时需预热培养基；收货如有大块脱落的细胞团，为正常现象，请按照收货注意事项处理。 |
| 背景描述 | 293 [HEK-293]细胞是剪切过的人腺病毒5(Ad5)转染的人胚肾细胞形成的永生化细胞，293 [HEK-293]细胞包含并表达转染的Ad5基因。早期报道中指出，293 [HEK-293]细胞基因组中含有腺病毒5(Ad5)基因组的左侧端和右侧端的DNA，但是现在明确了只存在其左侧端的DNA。经过对Ad5的插入点的克隆测序发现，Ad5的1-4344位线性核苷酸整合入293 [HEK-293]细胞19号染色体(19q13.2)。293 [HEK-293]细胞为人类腺病毒载体扩增的宿主，可表达异常的玻连蛋白的细胞表面受体，由整合素β1亚单位和玻连蛋白受体α-v亚单位组成。 |
| 年龄（性别） | 胎儿 |
| 组织来源 | 肾；以腺病毒5DNA进行转化 |
| 细胞类型 | 转化细胞系 |
| 生物安全等级 | BSL-2 |
| 倍增时间 | ~24-30 hours |
| 致瘤性 | Yes, forms tumors in nude mice. |
| 受体表达情况 | vitronectin |
| 保藏机构 | ATCC; CRL-1573 ATCC： PTA-4488 DSMZ： ACC-305 ECACC： 85120602 |
STR鉴定
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STR位点信息
Amelogenin X CSF1PO 12 D2S1338 19 D3S1358 15,17 D5S818 8 D7S820 11,12 D8S1179 12,14 D13S317 12,14 D16S539 9,13 D18S51 17,18 D19S433 15,16.2,18 D21S11 28,30.2 FGA 23 PentaD 9,10 PentaE 7,15 TH01 7,9.3 TPOX 11 vWA 16,19 D6S1043 11 D12S391 19,21 D2S441 11,15 -
STR鉴定图
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参考文献
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Landscape of pathogenic mutations in premature ovarian insufficiency(2023/02/02)
作者：Hanni Ke, Shuyan Tang, Ting Guo
期刊：NATURE MEDICINE
DOI：10.1038/s41591-022-02194-3
影响因子 ：82.900
-
Simulation guided intramolecular orthogonal reporters for dissecting cellular oxidative stress and response(2022/08/05)
作者：Yecan Pan, Rui Weng, Linghao Zhang
期刊：Nano Today
DOI：10.1016/j.nantod.2022.101573
影响因子 ：17.400
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ANGPTL8 negatively regulates NF-κB activation byfacilitating selective autophagic degradation ofIKKγ(2017/12/18)
作者：Yu Zhang, Xian Guo, Wanyao Yan
期刊：Nature Communications
DOI：10.1038/s41467-017-02355-w
影响因子 ：16.600
-
Gelatin-Encapsulated Tetrahedral DNA Nanostructure Enhances Cellular Internalization for Treating Noise-Induced Hearing Loss(2024/02/08)
作者：Ke Xu, Yiwei Du, Baoying Xu
期刊：Small
影响因子 ：13.300
引用产品：CL-0001
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Delivery of mRNA Vaccine with 1, 2-Diesters-Derived Lipids Elicits Fast Liver Clearance for Safe and Effective Cancer Immunotherapy(2023/11/21)
作者：Yuandong Xu, Yuexiao Hu, Heng Xia
期刊：Advanced Healthcare Materials
影响因子 ：10.000
引用产品：CL-0001
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Rational synthesis of carbon dots with phosphate ester group for direct mapping of endogenous alkaline phosphatase and polarity monitoring in living cells(2023/02/28)
作者：Yanzhi Xing, Yulong Xu, Yanna Lin
期刊：JOURNAL OF COLLOID AND INTERFACE SCIENCE
DOI：10.1016/j.jcis.2023.02.133
影响因子 ：9.900
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The novel protein complex FAPα/ITGA5 is involved in the bone destruction of apical periodontitis(2023/11/17)
作者：Shuo Liu, QianYang Chen, Lili Wang
期刊：INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
DOI：10.1016/j.ijbiomac.2023.128200
影响因子 ：8.200
引用产品：CL-0001
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Combined Doxorubicin Mesoporous Carbon Nanospheres for Effective Tumor Lymphatic Metastasis by Multi-Modal Chemo-Photothermal Treatment in vivo(2023/08/11)
作者：Yipengchen Yin, Jiaxin Sun, Tiaoyan Jiang
期刊：International Journal of Nanomedicine
影响因子 ：8.000
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Loss of renal tubular G9a benefits acute kidney injury by lowering focal lipid accumulation via CES1(2023/04/12)
作者：Dong Yang, Yu Fan, Mingrui Xiong
期刊：EMBO REPORTS
影响因子 ：7.700
引用产品：CL-0001
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Precise Detection on Cell–Cell Fusion by a Facile Molecular Beacon-Based Method(2022/12/01)
作者：Chang Xu, Xiaohe Ren, Di Han
期刊：Analytical Chemistry
DOI：10.1021/acs.analchem.2c04852
影响因子 ：7.400
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Discovery of Novel Coumarin-quinolinium Derivatives as Pan-KRAS Translation Inhibitors by Targeting 5′-UTR RNA G-Quadruplexes(2024/01/25)
作者：Maolin Li, Letian Dai, Zhuoyu Gao
期刊：JOURNAL OF MEDICINAL CHEMISTRY
DOI：10.1021/acs.jmedchem.3c01773
影响因子 ：7.300
引用产品：CL-0001
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Novel Functional Recombinant Human Follicle-Stimulating Hormone Acquired from Goat Milk(2021/03/01)
作者：Rongmao Hua, Jianxi Liu, Yuan Li
期刊：Journal Of Agricultural And Food Chemistry
影响因子 ：6.100
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Rapid selection of a novel GLP-1/GIP dual receptor agonist with prolonged glycemic control and weight loss in rodent animals(2020/6/26)
作者：Yumin Wu, Tiemei Ji, Jie Lv
期刊：Life Sciences
影响因子 ：6.100
引用产品：CL-0001
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Asparanin A from Asparagus officinalis L. Induces G0/G1 Cell Cycle Arrest and Apoptosis in Human Endometrial Carcinoma Ishikawa Cells via Mitochondrial and PI3K/AKT Signaling Pathways(2019/12/27)
作者：Fan Zhang, Yuanyuan Zhang, Yasai Sun
期刊：Journal Of Agricultural And Food Chemistry
影响因子 ：6.100
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miR-34a Regulates Lipid Droplet Deposition in 3T3-L1 and C2C12 Cells by Targeting LEF1(2022/12/30)
作者：Lixue Wang, Yuhuai Xie, Wei Chen
期刊：Cells
影响因子 ：6.000
-
Enhanced MiR-711 transcription by PPARγ induces endoplasmic reticulum stress-mediated apoptosis targeting calnexin in rat cardiomyocytes after myocardial infarction(2018/03/06)
作者：Na Zhao, Lan Mi, Xunjun Zhang
期刊：Journal Of Molecular And Cellular Cardiology
DOI：10.1016/j.yjmcc.2018.03.006
影响因子 ：5.763
引用产品：CL-0001
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BKCa channel participates in insulin‐induced lipid deposition in adipocytes by increasing intracellular calcium(2021/01/11)
作者：Jie Ren, Yuan Cheng, Xinxin Wen
期刊：Journal Of Cellular Physiology
影响因子 ：5.600
引用产品：CL-0001
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The extracellular loop of the auxiliary β1-subunit is involved in the regulation of BKCa channel mechanosensitivity(2018/9/14)
作者：Fang Xin, Yuan Cheng, Jie Ren
期刊：American Journal Of Physiology-Cell Physiology
DOI：10.1152/ajpcell.00037.2018
影响因子 ：5.500
引用产品：CL-0001
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Migration of Human Renal Tubular Epithelial Cells in Response to Physiological Electric Signals(2021/09/14)
作者：Linbo Guan, Ping Fan, Xinghui Liu
期刊：Frontiers in Cell and Developmental Biology
影响因子 ：5.500
-
DEPDC1B-mediated USP5 deubiquitination of β-catenin promotes breast cancer metastasis by activating the wnt/β-catenin pathway(2023/08/29)
作者：Qingqing Wang, Fengxia Chen, Ningning Yang
期刊：AMERICAN JOURNAL OF PHYSIOLOGY-CELL PHYSIOLOGY
DOI：10.1152/ajpcell.00249.2023
影响因子 ：5.500
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Bombyx batryticatus extract activates coagulation factor Ⅻ to promote angiogenesis in rats with cerebral ischemia/reperfusion injury(2023/08/25)
作者：Shanshan Wang, Hao Xu, Anqi Ge
期刊：JOURNAL OF ETHNOPHARMACOLOGY
影响因子 ：5.400
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Delta Opioid Peptide Targets Brain Microvascular Endothelial Cells Reducing Apoptosis to Relieve Hypoxia-Ischemic/Reperfusion Injury(2022/12/23)
作者：Ran Zhang, Meixuan Chen, Zhongfang Deng
期刊：Pharmaceutics
DOI：10.3390/pharmaceutics15010046
影响因子 ：5.400
引用产品：CL-0001
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lncRNA DGCR 5/miR‑27a‑3p/BNIP3 promotes cell apoptosis in pancreatic cancer by regulating the p38 MAPK pathway(2020/06/04)
作者：Xianjie Li, Shanxue Zhou, Tianyi Fan
期刊：International Journal of Molecular Medicine
影响因子 ：5.400
引用产品：CL-0001
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Exosome‑encapsulated miR‑26a attenuates aldosterone‑induced tubulointerstitial fibrosis by inhibiting the CTGF/SMAD3 signaling pathway(2022/12/12)
作者：Hui Zheng, Jialing Ji, Tangming Zhao
期刊：International Journal Of Molecular Medicine
影响因子 ：5.400
引用产品：CL-0001
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Integrated miRNA and mRNA omics reveal dioscin suppresses migration and invasion via MEK/ERK and JNK signaling pathways in human endometrial carcinoma in vivo and in vitro(2022/12/09)
作者：Xiaoli Li, Xiuxiu Zhang, Runhui Ma
期刊：JOURNAL OF ETHNOPHARMACOLOGY
影响因子 ：5.400
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Antibacterial and Antifungal Properties of a Novel Antimicrobial Peptide GK-19 and Its Application in Skin and Soft Tissue Infections Induced by MRSA or Candida albicans(2022/09/13)
作者：Chenghua Song, Ruichao Wen, Jiaxuan Zhou
期刊：Pharmaceutics
DOI：10.3390/pharmaceutics14091937
影响因子 ：5.400
引用产品：CL-0001
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FXII regulates the formation of deep vein thrombosis via the PI3K/AKT signaling pathway in mice(2021/03/23)
作者：Yan Meng, Qian Yin, Qiang Ma
期刊：International Journal Of Molecular Medicine
影响因子 ：5.314
引用产品：CL-0001
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Characterization of the metabolites of Eucommiae Cortex in rats provides a further insight into its estrogen-like effective substances(2023/12/20)
作者：Liqin Ding, Tianyi Qiu, Yanwen Sun
期刊：BIOORGANIC CHEMISTRY
DOI：10.1016/j.bioorg.2023.107052
影响因子 ：5.100
引用产品：CL-0001
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Silencing circular RNAPTPN12 promoted the growth of keloid fibroblasts by activating Wnt signaling pathway via targeting microRNA-21-5p(2022/01/22)
作者：Fei Liu, Tao Li, Xiaoan Zhan
期刊：Bioengineered
DOI：10.1080/21655979.2022.2029108
影响因子 ：4.900
引用产品：CL-0001
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Ubiquitin ligase Triad1 promotes neurite outgrowth by inhibiting MDM2-mediated ubiquitination of the neuroprotective factor pleiotropin(2022/08/31)
作者：Chunshuai Wu, Guanhua Xu, Guofeng Bao
期刊：JOURNAL OF BIOLOGICAL CHEMISTRY
影响因子 ：4.800
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Metal-bridged DNA-functionalized silica nanoparticles for multifacet biological applications(2021/11/28)
作者：Yue Huang, Jiehua Ma, Yuting Yan
期刊：Microchemical Journal
DOI：10.1016/j.microc.2021.107017
影响因子 ：4.800
引用产品：CL-0001
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Supramolecular Switch for the Regulation of Antibacterial Efficacy of Near-Infrared Photosensitizer(2024/02/28)
作者：Yuna Jiang, Manqi Tan, Chenglong He
期刊：MOLECULES
影响因子 ：4.600
引用产品：CL-0001
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Whole genome sequencing identifies a deletion mutation in the unknown-functional KCNG2 from familial sick sinus syndrome(2022/04/01)
作者：Chen Sun, Ning Li, Qianqian Wang
期刊：PHYSIOLOGICAL GENOMICS
DOI：10.1152/physiolgenomics.00132.2021
影响因子 ：4.600
引用产品：CL-0001
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Associations of Gut and Circulating Microbiota with Circulating Vitamin D3, Type I Interferon, and Systemic Inflammation in Chronic Spontaneous Urticaria Patients(2024/05/06)
作者：Zhi Yang, Yao Song, Bangtao Chen
期刊：Journal of Inflammation Research
影响因子 ：4.500
引用产品：CL-0001
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Silencing ApoC3 alleviates LPS-induced acute lung injury by inhibiting TLR signaling pathway(2023/04/10)
作者：Yongjie Qi, Chen Chen, Xuejun Li
期刊：IMMUNOLOGIC RESEARCH
DOI：10.1007/s12026-023-09379-z
影响因子 ：4.400
引用产品：CL-0001
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Cancer stem cell-like cells-derived exosomal lncRNA CDKN2B-AS1 promotes biological characteristics in thyroid cancer via miR-122-5p/P4HA1 axis(2022/12/14)
作者：Qinghua Wu, Yonggang He, Xin Liu
期刊：Regenerative Therapy
DOI：10.1016/j.reth.2022.11.005
影响因子 ：4.300
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DNA nanolantern-based split aptamer probes for in situ ATP imaging in living cells and lighting up mitochondria(2021/03/02)
作者：Yaxin Wang, Dongxia Wang, Jiayi Ma
期刊：Analyst
影响因子 ：4.200
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Glioma-Derived Exosome Lncrna Agap2-As1 Promotes Glioma Proliferation and Metastasis By Mediating Tgf-β1 Secretion of Myeloid-Derived Suppressor Cells(2024/04/18)
作者：Yanlong Tian, Xiao Gao, Xuechao Yang
期刊：Heliyon
DOI：10.1016/j.heliyon.2024.e29949
影响因子 ：4.000
引用产品：CL-0001
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Preparation of porous calcium phosphate microspheres with phosphate-containing molecules at room temperature for drug delivery and osteogenic differentiation(2018/7/16)
作者：Junfeng Liu, Lu Wei, Dilixiati Duolikun
期刊：RSC Advances
影响因子 ：3.900
引用产品：CL-0001
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Effects of Lycium barbarum glycopeptide on renal and testicular injury induced by di(2-ethylhexyl) phthalate(2022/04/01)
作者：Xianling Zhou, Zhigang Zhang, Heng Shi
期刊：CELL STRESS & CHAPERONES
DOI：10.1007/s12192-022-01266-0
影响因子 ：3.800
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YBX2 modulates mRNA stability via interaction with YTHDF2 in endometrial cancer cells(2023/04/06)
作者：Ying Cai, Na Li, Huaibiao Li
期刊：EXPERIMENTAL CELL RESEARCH
DOI：10.1016/j.yexcr.2023.113586
影响因子 ：3.700
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Antitumor effect of Escherichia coli-derived outer membrane vesicles on neuroblastoma in vitro and in vivo : Effect of E. coli-OMVs on neuroblastoma(2022/09/20)
作者：Liming Jin, Zhaoxia Zhang, Xiaojun Tan
期刊：ACTA BIOCHIMICA ET BIOPHYSICA SINICA
影响因子 ：3.511
引用产品：CL-0001
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ADAR3 alleviated inflammation and pyroptosis of neuropathic pain by targeting NLRP3 in chronic constriction injury mice(2021/08/19)
作者：Zongji Li, Jiajia Zhu, Yin Wang
期刊：Gene
DOI：10.1016/j.gene.2021.145909
影响因子 ：3.500
引用产品：CL-0001
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Bta-miR-106b Regulates Bovine Mammary Epithelial Cell Proliferation, Cell Cycle, and Milk Protein Synthesis by Targeting the CDKN1A Gene(2022/12/07)
作者：Xin Wu, Jinfeng Huang, Yanan Liu
期刊：Genes
影响因子 ：3.500
引用产品：CL-0001
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NCK1-AS1 Increases Drug Resistance of Glioma Cells to Temozolomide by Modulating miR-137/TRIM24(2020/03/05)
作者：Mingsheng Chen, Yingying Cheng, Zhihai Yuan
期刊：CANCER BIOTHERAPY AND RADIOPHARMACEUTICALS
影响因子 ：3.400
引用产品：CL-0001
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KLF5-mediated aquaporin 3 activated autophagy to facilitate cisplatin resistance of gastric cancer(2022/09/09)
作者：Xudong Dai, Yong Chen, Ning Chen
期刊：IMMUNOPHARMACOLOGY AND IMMUNOTOXICOLOGY
DOI：10.1080/08923973.2022.2122498
影响因子 ：3.300
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LncPVT1 promotes cartilage degradation in diabetic OA mice by downregulating miR-146a and activating TGF-β/SMAD4 signaling(2021/02/10)
作者：Yanzhi Wang, Yaoli, Shengkai Liang
期刊：Journal of Bone and Mineral Metabolism
DOI：10.1007/s00774-020-01199-7
影响因子 ：3.300
引用产品：CL-0001
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LINC00839 Regulates Proliferation, Migration, Invasion, Apoptosis and Glycolysis in Neuroblastoma Cells Through miR-338-3p/GLUT1 Axis(2021/6/21)
作者：Lixia Yang, Liangyan Pei, Jilong Yi
期刊：Neuropsychiatric Disease and Treatment
影响因子 ：3.200
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Progesterone Changes the Pregnancy-Induced Adaptation of Cardiomyocyte Kv2.1 Channels via MicroRNA-29b(2022/04/07)
作者：Shuang Liang, Yushuang Sun, Lu Li
期刊：Cardiovascular Therapeutics
影响因子 ：3.100
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Inhibition of Kv7/M Channel Currents by Fangchinoline(2022/12/14)
作者：Han Li, DanDan Geng, Rong Zheng
期刊：PHARMACOLOGY
影响因子 ：3.100
引用产品：CL-0001
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CircHDAC9 regulates myocardial ischemia-reperfusion injury via miR-671-5p/SOX4 signaling axis(2023/11/07)
作者：Qin Liu, Yanhui Hu, Huanhuan Jie
期刊：AMERICAN JOURNAL OF THE MEDICAL SCIENCES
DOI：10.1016/j.amjms.2023.11.001
影响因子 ：3.100
引用产品：CL-0001
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LINC00839 Regulates Proliferation, Migration, Invasion, Apoptosis and Glycolysis in Neuroblastoma Cells Through miR-338-3p/GLUT1 Axis(2022/12/19)
作者：Lixia Yang, Liangyan Pei, Jilong Yi
期刊：Neuropsychiatric Disease and Treatment
影响因子 ：2.989
引用产品：CL-0001
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Circ_0116061 regulated the proliferation, apoptosis, and inflammation of osteoarthritis chondrocytes through regulating the miR-200b-3p/SMURF2 axis(2021/4/13)
作者：Wei Zheng, Guanhua Hou, Yong Li
期刊：Journal of Orthopaedic Surgery and Research
DOI：10.1186/s13018-021-02391-9
影响因子 ：2.600
引用产品：CL-0001
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IRF1 promotes the chondrogenesis of human adipose-derived stem cells through regulating HILPDA(2023/02/21)
作者：Yujun Zhao, Xiaotie Wang, Keke Nie
期刊：TISSUE & CELL
DOI：10.1016/j.tice.2023.102046
影响因子 ：2.600
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Proinflammatory Bone Marrow Mesenchymal Stem Cell-Derived Exosomal miR-150-3p Suppresses Proinflammatory Polarization of Alveolar Macrophages in Sepsis by Targeting Inhibin Subunit Beta A(2023/10/11)
作者：Guojin Liang, Yueying Feng, Wan Tang
期刊：JOURNAL OF INTERFERON AND CYTOKINE RESEARCH
影响因子 ：2.300
引用产品：CL-0001
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Elevated histone deacetylase 10 expression promotes the progression of clear cell renal cell carcinoma by Notch-1-PTEN signaling axis(2024/05/11)
作者：Bin Zheng, Xue Jiang, Yaqing Liu
期刊：Discover Oncology
DOI：10.1007/s12672-024-01018-9
影响因子 ：2.200
引用产品：CL-0001
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Long Non-Coding RNA CASC2 Overexpression Ameliorates Sepsis-Associated Acute Kidney Injury by Regulating MiR-545-3p/PPARA axis(2021/05/03)
作者：Qionghua Hu, Weiwei Zen, Ming Zhang
期刊：Journal of Surgical Research
影响因子 ：2.200
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LPS-induced PTGS2 manipulates the inflammatory response through trophoblast invasion in preeclampsia via NF-κB pathway(2022/10/31)
作者：Ruolan Li, Jirong Xie, Wei Xu
期刊：Reproductive Biology
DOI：10.1016/j.repbio.2022.100696
影响因子 ：2.100
引用产品：CL-0001
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