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产品概述
| 名称 | B16-F10 (小鼠皮肤黑色素瘤细胞) (STR鉴定正确) |
| 别称 | B16/F10; B16 F10; B16F10; B16 melanoma F10 |
| 种属 | 小鼠 |
| 生长特性 | 贴壁细胞 |
| 细胞形态 | 梭形细胞样；上皮样细胞样 |
| 冻存条件 | 冻存液：55% 基础培养基+40%FBS+5%DMSO 温度：液氮 |
| 培养方案A（默认） |
培养条件:
气相：空气，95%；CO2，5%, 温度：37℃
|
| 推荐传代比例 | 1:3-1:4 |
| 推荐换液频率 | 2-3次/周 |
| 注意事项 | B16系列细胞，使用DMEM培养时可能出现黑色素快速积累，细胞不增殖或死亡的情况，该细胞正常扩增冻存请使用RPMI-1640完培，若您需要进行分泌黑色素等后续相关实验，可以在扩增冻存细胞后更换为DMEM培养来满足实验设计。 |
| 背景描述 | B16-F10细胞是B16-F0细胞的亚系。 |
| 年龄（性别） | 不详 |
| 组织来源 | 器官：皮肤；品系：C57BL/6J；疾病：黑色素瘤 |
| 细胞类型 | 肿瘤细胞 |
| 肿瘤类型 | 黑色素瘤细胞 |
| 生物安全等级 | BSL-1 |
| 保藏机构 | ATCC; CRL-6475 |
STR鉴定
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STR位点信息
18-3 15,16 6-7 15 5-5 16,20 X-1 28 1-2 19,20 7-1 26.2 8-1 16,17 1-1 17,18,19 19-2 13,14 15-3 22.3,23.3 12-1 17,18 6-4 18,19 4-2 20.3,21.3 3-2 14,15 2-1 16 13-1 17.1,18.1
11-2 16,17 17-2 15,16,17 -
STR鉴定图
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参考文献
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Engineered extracellular vesicles enable high-efficient delivery of intracellular therapeutic proteins(2024/03/22)
作者：Ding Ma, An Xie, Jiahui Lv
期刊：Protein & Cell
影响因子 ：21.100
引用产品：CL-0319
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A self-monitoring microneedle patch for light-controlled synergistic treatment of melanoma(2023/03/27)
作者：Chenyuan Wang, Yongnian Zeng, Kaifeng Chen
期刊：Bioactive Materials
DOI：10.1016/j.bioactmat.2023.03.016
影响因子 ：18.900
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An all-in-one tetrazine reagent for cysteine-selective labeling and bioorthogonal activable prodrug construction(2024/04/02)
作者：Xinyu He, Jie Li, Xinxin Liang
期刊：Nature Communications
DOI：10.1038/s41467-024-47188-6
影响因子 ：16.600
引用产品：CL-0319
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Mn-CaCO3-based nanosystem for augmented sonodynamic-chemodynamic immunotherapy via PI3K/Akt signaling pathway(2024/02/09)
作者：Mashiti Sha, Huansong Li, Yangsui Liu
期刊：CHEMICAL ENGINEERING JOURNAL
影响因子 ：15.100
引用产品：CL-0319
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Establishment of tumor inflammasome clusters with distinct immunogenomic landscape aids immunotherapy(2021/10/17)
作者：Qingyu Liang, Jianqi Wu, Xin Zhao
期刊：Theranostics
影响因子 ：12.400
引用产品：CL-0319
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Enzyme Responsiveness Enhances the Specificity and Effectiveness ofNanoparticles for the Treatment of B16F10 Melanoma(2019/11/02)
作者：Yi Huang, Yanbin Shi, Qingjie Wang
期刊：Journal Of Controlled Release
DOI：10.1016/j.jconrel.2019.10.052
影响因子 ：10.800
引用产品：CL-0319
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Nanotechnology-mediated immunochemotherapy combined with docetaxel and PD-L1 antibody increase therapeutic effects anddecrease systemic toxicity(2018/08/07)
作者：Zili Gu, Qingjie Wang, Yanbin Shi
期刊：Journal Of Controlled Release
DOI：10.1016/j.jconrel.2018.08.011
影响因子 ：10.800
引用产品：CL-0319
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Suppression of Tumor or Host Intrinsic CMTM6 Drives Antitumor Cytotoxicity in a PD-L1–Independent Manner(2023/02/03)
作者：Yiru Long, Runqiu Chen, Xiaolu Yu
期刊：Cancer Immunology Research
DOI：10.1158/2326-6066.CIR-22-0439
影响因子 ：10.100
引用产品：CL-0005 CL-0007 CL-0019 CL-0071 CL-0105 CL-0129 CL-0140 CL-0196 CL-0246 CL-0319
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A novel mitochondria-targeting DHODH inhibitor induces robust ferroptosis and alleviates immune suppression(2024/02/27)
作者：Yongrui Hai, Renming Fan, Ting Zhao
期刊：PHARMACOLOGICAL RESEARCH
DOI：10.1016/j.phrs.2024.107115
影响因子 ：9.300
引用产品：CL-0319
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Skin healthcare protection with antioxidant and anti-melanogenesis activity of polysaccharide purification from Bletilla striata(2024/02/15)
作者：Haoying Chen, Yan Wu, Bin Wang
期刊：INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
DOI：10.1016/j.ijbiomac.2024.130016
影响因子 ：8.200
引用产品：CL-0319
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MC1R-targeted NIR-II aggregation-induced emission nanoparticles for melanoma imaging(2023/09/21)
作者：Zhaoying Liu, Shurui Pei, Kaikai Wen
期刊：Science China-Materials
影响因子 ：8.100
引用产品：CL-0319
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Engineered in vivo and in vitro tumor model recapitulates vasculogenic mimicry signatures in melanoma(2024/01/27)
作者：Qizhi Shuai, Xinrui Xu, Yuxiang Liang
期刊：Bioengineering & Translational Medicine
影响因子 ：7.400
引用产品：CL-0319
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M1 polarization enhances the antitumor activity of chimeric antigen receptor macrophages in solid tumors(2023/03/28)
作者：Yi Huo, Han Zhang, Longqi Sa
期刊：Journal of Translational Medicine
DOI：10.1186/s12967-023-04061-2
影响因子 ：7.400
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RNF8 enhances the sensitivity of PD-L1 inhibitor against melanoma through ubiquitination of galectin-3 in stroma(2023/06/30)
作者：Yanan Guo, Rong Shen, Keren Yang
期刊：Cell Death Discovery
DOI：10.1038/s41420-023-01500-3
影响因子 ：7.000
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MRI-guided photothermal/photodynamic immune activation combined with PD-1 inhibitor for the multimodal combination therapy of melanoma and metastases(2024/03/14)
作者：Changqiang Wu, Wei Chen, Shuang Yan
期刊：Regenerative Biomaterials
影响因子 ：6.700
引用产品：CL-0319
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Thermosensitive Polymer Conjugated Prodrug-Activating Enzyme with Enhanced Tumor Retention and Antitumor Efficacy(2022/10/20)
作者：Xiang Zhang, Fei Duan, Fan Zhang
期刊：BIOMACROMOLECULES
DOI：10.1021/acs.biomac.2c01006
影响因子 ：6.200
引用产品：CL-0319
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Effects of novel cellulase (Cel 906) and probiotic yeast fermentation on antioxidant and anti-inflammatory activities of vine tea (Ampelopsis grossedentata).(2022/09/15)
作者：Jin Xu, Mubasher Hussain, Wenfeng Su
期刊：Frontiers in Bioengineering and Biotechnology
DOI：10.3389/fbioe.2022.1006316
影响因子 ：5.700
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Metabolites 13,14-Dihydro-15-keto-PGE2 Participates in Bifidobacterium animalis F1-7 to Alleviate Opioid-Induced Constipation by 5-HT Pathway(2023/12/06)
作者：Youyou Lu, Xiaohong Zhou, Yeting Wu
期刊：MOLECULAR NUTRITION & FOOD RESEARCH
影响因子 ：5.200
引用产品：CL-0319
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Linear Peptide-Based PET Tracers for Imaging PD-L1 in Tumors(2023/06/27)
作者：Lulu Zhang, Siqi Zhang, Jiang Wu
期刊：MOLECULAR PHARMACEUTICS
DOI：10.1021/acs.molpharmaceut.3c00382
影响因子 ：4.900
引用产品：CL-0319
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Bifidobacterium animalis sup F1-7 Acts as an Effective Activator to Regulate Immune Response Via Casepase-3 and Bak of FAS/CD95 Pathway(2022/08/22)
作者：Youyou Lu, Xi Liang, Yeting Wu
期刊：Probiotics and Antimicrobial Proteins
DOI：10.1007/s12602-022-09975-9
影响因子 ：4.900
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Identification and characterization of two novel non-coding Tyrosinase (TYR) gene variants leading to Oculocutaneous albinism type 1(2022/04/10)
作者：Chaoyi Li, Qian Chen, Junjiao Wu
期刊：JOURNAL OF BIOLOGICAL CHEMISTRY
影响因子 ：4.800
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Screening of Key Components for Melanogenesis Inhibition of Polygonum cuspidatum Extract Based on the Spectrum–Effect Relationship and Molecular Docking(2024/02/15)
作者：Ruojun Du, Lichun Ye, Xinyan Chen
期刊：MOLECULES
影响因子 ：4.600
引用产品：CL-0319
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Fractionation of Simulated Digestive Products of Bird’s Nest In Vitro: Skin Whitening Activity of Fractions(2023/08/25)
作者：Haili Yi, Meizi Piao, Qianru Chen
期刊：JOURNAL OF FOOD BIOCHEMISTRY
影响因子 ：4.000
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Nonspecific immunoglobulin G is effective in preventing and treating cancer in mice(2019/03/07)
作者：Qian Xu, Zaiping Zhang, Zhiming Chen
期刊：Cancer Management and Research
影响因子 ：3.300
引用产品：CL-0319
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Neospora caninum inhibits tumor development by activating the immune response and destroying tumor cells in a B16F10 melanoma model(2022/09/23)
作者：Xiaojin Li, Meng Qi, Kai He
期刊：Parasites & Vectors
DOI：10.1186/s13071-022-05456-8
影响因子 ：3.200
引用产品：CL-0319
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Licorice zinc suppresses melanogenesis via inhibiting the activation of P38MAPK and JNK signaling pathway in C57BL/6J mice skin(2022/12/19)
作者：Jingyan Wang, Xingyu Xie, Ying Deng
期刊：Acta Cirurgica Brasileira
影响因子 ：1.100
引用产品：CL-0319
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