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![NCI-H460 [H460] (人大细胞肺癌细胞) (STR鉴定正确)](/Public/upfile/pic/xbx/CL-0299-1.jpg)
![NCI-H460 [H460] (人大细胞肺癌细胞) (STR鉴定正确)](/Public/upfile/pic/xbx/CL-0299-2.jpg)
![NCI-H460 [H460] (人大细胞肺癌细胞) (STR鉴定正确)](/Public/upfile/pic/xbx/CL-0299-3.jpg)
![NCI-H460 [H460] (人大细胞肺癌细胞) (STR鉴定正确)](/Public/upfile/pic/xbx/CL-0299-4.jpg)
![NCI-H460 [H460] (人大细胞肺癌细胞) (STR鉴定正确)](/Public/upfile/pic/xbx/CL-0299-5.jpg)
![NCI-H460 [H460] (人大细胞肺癌细胞) (STR鉴定正确)](/Public/upfile/pic/xbx/CL-0299-6.jpg)
![NCI-H460 [H460] (人大细胞肺癌细胞) (STR鉴定正确)](/Public/upfile/pic/product/cell-line-new.jpg)
产品概述
| 名称 | NCI-H460 [H460] (人大细胞肺癌细胞) (STR鉴定正确) |
| 别称 | NCI.H460; H460; H-460; NCIH460; NCI-HUT-460; NCI-460 |
| 种属 | 人 |
| 生长特性 | 贴壁细胞 |
| 细胞形态 | 上皮细胞样 |
| 冻存条件 | 冻存液：55% 基础培养基+40%FBS+5%DMSO 温度：液氮 |
| 培养方案A（默认） |
培养条件:
气相：空气，95%；CO2，5%, 温度：37℃
|
| 推荐传代比例 | 1:3-1:4 |
| 推荐换液频率 | 2-3次/周 |
| 背景描述 | NCI-H460细胞是从一位大细胞肺癌患者的胸水中建立的。NCI-H460细胞表达的p53 mRNA易于检测，其水平与正常肺细胞相当，未表现出NCI-H460细胞DNA总体结构异常。NCI-H460细胞角蛋白和波形蛋白纤维染色阳性；神经丝三联体蛋白阴性。 |
| 年龄（性别） | 男性 |
| 组织来源 | 肺；转移灶：肋膜渗出癌；大细胞肺癌 |
| 细胞类型 | 肿瘤细胞 |
| 肿瘤类型 | 肺癌细胞 |
| 生物安全等级 | BSL-1 |
| 倍增时间 | ~23-60 hours |
| 致瘤性 | Yes, in nude mice. |
| 保藏机构 | ATCC; HTB-177 DSMZ; ACC-737 |
STR鉴定
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STR位点信息
Amelogenin X,Y CSF1PO 11,12 D2S1338 17,25 D3S1358 15,18 D5S818 9,10 D7S820 9,12 D8S1179 12 D13S317 13 D16S539 9 D18S51 13,15 D19S433 14 D21S11 30 FGA 21,23 PentaD 11,13 PentaE 5 TH01 9.3 TPOX 8 vWA 17 D6S1043 11,14 D12S391 21 D2S441 11,11.3 -
STR鉴定图
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参考文献
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LINC00173.v1 Promotes Angiogenesis and Progression of Lung Squamous Cell Carcinoma by Sponging miR-511-5p to Regulate VEGFA Expression(2020/07/10)
作者：Aibin Liu
期刊：Molecular Cancer
DOI：10.1186/s12943-020-01217-2
影响因子 ：37.300
-
The p23 co-chaperone is a succinate-activated COX-2 transcription factor in lung adenocarcinoma tumorigenesis(2023/06/30)
作者：Zhenlong Yu, Yulin Peng, Jian Gao
期刊：Science Advances
影响因子 ：13.600
-
Tumor-suppressive miR-4732-3p is sorted into fucosylated exosome by hnRNPK to avoid the inhibition of lung cancer progression(2024/04/23)
作者：Wanzhen Zhuang, Chengxiu Liu, Yilin Hong
期刊：JOURNAL OF EXPERIMENTAL & CLINICAL CANCER RESEARCH
DOI：10.1186/s13046-024-03048-1
影响因子 ：11.300
引用产品：CL-0299
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USP7 promotes non‐small‐cell lung cancer cell glycolysis and survival by stabilizing and activating c‐Abl(2023/12/11)
作者：Yuanming He, Shuoyi Jiang, Yueya Zhong
期刊：Clinical and Translational Medicine
影响因子 ：10.600
引用产品：CL-0299
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Discovery of a nitroaromatic nannocystin with potent in vivo anticancer activity against colorectal cancer by targeting AKT1(2024/02/07)
作者：Han Zhang, Fei Xie, Xiaoya Yuan
期刊：ACTA PHARMACOLOGICA SINICA
DOI：10.1038/s41401-024-01231-w
影响因子 ：8.200
引用产品：CL-0299
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TRIM25 activates AKT/mTOR by inhibiting PTEN via K63-linked polyubiquitination in non-small cell lung cancer(2021/04/30)
作者：Yuanming He, Xiumin Zhou, Shuoyi Jiang
期刊：Acta Pharmacologica Sinica
DOI：10.1038/s41401-021-00662-z
影响因子 ：8.200
-
Curcumin suppresses the malignancy of non-small cell lung cancer by modulating the circ-PRKCA/miR-384/ITGB1 pathway(2021/03/06)
作者：Xiaoqing Xu, Xinyue Zhang, Yang Zhang
期刊：Biomedicine & Pharmacotherapy
DOI：10.1016/j.biopha.2021.111439
影响因子 ：7.500
引用产品：164210 164210-50 CL-0005 CL-0016 CL-0165 CL-0166 CL-0299 PB180120 PM150110
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Discovery of a Potent, Cooperative, and Selective SOS1 PROTAC ZZ151 with In Vivo Antitumor Efficacy in KRAS-Mutant Cancers(2023/03/10)
作者：Zehui Zhou, Guizhen Zhou, Chuan Zhou
期刊：JOURNAL OF MEDICINAL CHEMISTRY
DOI：10.1021/acs.jmedchem.3c00075
影响因子 ：7.300
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A subset of VEGFR‐TKIs activates AMPK in LKB1‐mutant lung cancer(2022/12/18)
作者：Lujie Yang, Qin Zhang, Yanli Xiong
期刊：CANCER SCIENCE
影响因子 ：6.518
引用产品：CL-0299
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Long non‑coding RNA lung cancer‑associated transcript 1 regulates ferroptosis via microRNA‑34a‑5p‑mediated GTP cyclohydrolase 1 downregulation in lung cancer cells(2024/05/10)
作者：Fumin Tai, Rui Zhai, Kexin Ding
期刊：INTERNATIONAL JOURNAL OF ONCOLOGY
影响因子 ：5.200
引用产品：CL-0299
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Hsa_circ_0006677 regulates special AT-rich binding protein-2-mediated tumor-suppressive effect via functioning as a miR-1245a sponge in non-small cell lung cancer(2022/01/26)
作者：Xizhong Sui, Zongzhi Liu, Lei Niu
期刊：Bioengineered
DOI：10.1080/21655979.2022.2031400
影响因子 ：4.900
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Hsa_circ_0017639 regulates cisplatin resistance and tumor growth via acting as a miR-1296-5p molecular sponge and modulating sine oculis homeobox 1 expression in non-small cell lung cancer(2022/03/28)
作者：Feiyun Chang , Jiali Li , Quan Sun
期刊：Bioengineered
DOI：10.1080/21655979.2022.2053810
影响因子 ：4.900
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Exosome-transmitted S100A4 induces immunosuppression and non-small cell lung cancer development by activating STAT3(2022/11/12)
作者：Wu Xu, Zhang Hui, Jiang Gang, Peng Minlian, Li Cheng, Lu Jiaxin, Jiang Shiyin, Yang Xiaoping, Jiang Yongliang
期刊：Clinical And Experimental Immunology
影响因子 ：4.600
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Tandem CAR-T cells targeting MUC1 and PSCA combined with anti-PD-1 antibody exhibit potent preclinical activity against non-small cell lung cancer(2023/08/29)
作者：Aying Wang, Tangfeng Lv, Yong Song
期刊：CELLULAR IMMUNOLOGY
DOI：10.1016/j.cellimm.2023.104760
影响因子 ：4.300
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Cancer-associated fibroblasts-derived exosomal METTL3 promotes the proliferation, invasion, stemness and glutaminolysis in non-small cell lung cancer cells by eliciting SLC7A5 m6A modification(2024/04/16)
作者：Yafeng Fan, Yanling Yu
期刊：Human Cell
DOI：10.1007/s13577-024-01056-z
影响因子 ：4.300
引用产品：CL-0299
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Design, synthesis and biological evaluation of rhein–piperazine–furanone hybrids as potential anticancer agents(2024/02/13)
作者：Yu He, Sisi Zhang, Mengxue Wei
期刊：RSC Medicinal Chemistry
影响因子 ：4.100
引用产品：CL-0299
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Circ-SATB2 (hsa_circ_0008928) and miR-150-5p are regulators of TRIM66 in the regulation of NSCLC cell growth and metastasis of NSCLC cells via the ceRNA pathway(2023/12/12)
作者：Liangji Chen, Yuting Zhou, Hongbing Cheng
期刊：JOURNAL OF BIOCHEMICAL AND MOLECULAR TOXICOLOGY
影响因子 ：3.600
引用产品：CL-0299
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Role and mechanism of COL3A1 in regulating the growth, metastasis, and drug sensitivity in cisplatin-resistant non-small cell lung cancer cells(2024/03/26)
作者：Jiankun Ren, Songwei Zhao, Junyu Lai
期刊：CANCER BIOLOGY & THERAPY
DOI：10.1080/15384047.2024.2328382
影响因子 ：3.600
引用产品：CL-0299
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LINC00115 regulates lung adenocarcinoma progression via sponging miR-154-3p to modulate Sp3 expression(2023/03/16)
作者：Kexin Sun, Tingting Lu, Cheng Hu
期刊：MOLECULAR AND CELLULAR PROBES
影响因子 ：3.300
引用产品：CL-0299
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Hsa_circ_0003220 Drives Chemoresistance of Human NSCLC Cells by Modulating miR-489-3p/IGF1(2023/06/16)
作者：Shaofeng Xia, Chenliang Wang
期刊：International Journal of Genomics
影响因子 ：2.900
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Andrographis modulates cisplatin resistance in lung cancer via miR-155-5p/SIRT1 axis(2023/08/02)
作者：Chong Pang, Tengyue Zhang, Yulong Chen
期刊：FUNCTIONAL & INTEGRATIVE GENOMICS
DOI：10.1007/s10142-023-01186-x
影响因子 ：2.900
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The Potential Mechanism of HDAC1-Catalyzed Histone Crotonylation of Caspase-1 in Nonsmall Cell Lung Cancer(2022/08/02)
作者：Chuan Jiang, Jiangrong Liao, Fang Yang
期刊：Evidence-based Complementary and Alternative Medicine
影响因子 ：2.650
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Circular RNA (circ)_0053277 Contributes to Colorectal Cancer Cell Growth, Angiogenesis, Metastasis and Glycolysis(2023/11/02)
作者：Jianbin Zhuang, Weiliang Song, Minghao Li
期刊：MOLECULAR BIOTECHNOLOGY
DOI：10.1007/s12033-023-00936-3
影响因子 ：2.600
引用产品：CL-0299
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Serum Hsa_circ_0005962 Is A Prognostic Biomarker of Paclitaxel Resistance in Nonsmall Cell Lung Cancer Treatment(2023/10/19)
作者：Chenliang Wang, Shaofeng Xia
期刊：INTERNATIONAL JOURNAL OF CLINICAL PRACTICE
影响因子 ：2.600
引用产品：CL-0299
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Antioncogenic roles of USP9Y and DDX3Y in lung cancer: USP9Y stabilizes DDX3Y by preventing its degradation through deubiquitination(2024/01/12)
作者：Lei Xiu, Bo Ma, Lili Ding
期刊：ACTA HISTOCHEMICA
DOI：10.1016/j.acthis.2023.152132
影响因子 ：2.500
引用产品：CL-0299
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Circ_0079530 stimulates THBS2 to promote the malignant progression of non-small cell lung cancer by sponging miR-584-5p(2023/06/01)
作者：Kun Fang, Yibin Deng, Ping Yang
期刊：HISTOLOGY AND HISTOPATHOLOGY
影响因子 ：2.000
引用产品：CL-0299
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Hsa_circ_0003489 Drives PTX Resistance of Human NSCLC Cells Through Modulating miR-98-5p/IGF2(2023/09/04)
作者：Shaofeng Xia, Chenliang Wang
期刊：Pharmacogenomics & Personalized Medicine
影响因子 ：1.900
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Circ_0030411 aggravates cisplatin-resistance in non-small cell lung cancer by serving as a miR-495-3p sponge to enhance CCND1 expression(2023/01/02)
作者：Jing Zhang, Xiaohai Cui, Hangying Qu
期刊：JOURNAL OF CHEMOTHERAPY
DOI：10.1080/1120009X.2022.2162218
影响因子 ：1.800
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Novel macamides from maca (Lepidium meyenii Walpers) root and their cytotoxicity(2018/03/16)
作者：Chen Xia, Jian Chen, Jun-Lin Deng
期刊：Phytochemistry Letters
DOI：10.1016/j.phytol.2018.03.001
影响因子 ：1.700
引用产品：CL-0299
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Synergistic anti-tumor effects of lenalidomide and gefitinib by upregulating ADRB2 and inactivating the mTOR/PI3K/AKT signaling pathway in lung adenocarcinoma(2024/02/29)
作者：Shuangshuang Zhou, Meiyi Deng, Xuyu Bian
期刊：CELLULAR AND MOLECULAR BIOLOGY
影响因子 ：1.600
引用产品：CL-0299
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