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![SW480 [SW-480] (人结肠癌细胞) (STR鉴定正确)](/Public/upfile/pic/xbx/CL-0223-1.jpg)
![SW480 [SW-480] (人结肠癌细胞) (STR鉴定正确)](/Public/upfile/pic/xbx/CL-0223-2.jpg)
![SW480 [SW-480] (人结肠癌细胞) (STR鉴定正确)](/Public/upfile/pic/xbx/CL-0223-3.jpg)
![SW480 [SW-480] (人结肠癌细胞) (STR鉴定正确)](/Public/upfile/pic/xbx/CL-0223-4.jpg)
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产品概述
| 名称 | SW480 [SW-480] (人结肠癌细胞) (STR鉴定正确) |
| 别称 | SW-480; SW 480; SW480E |
| 种属 | 人类 |
| 生长特性 | 贴壁细胞 |
| 细胞形态 | 上皮细胞样 |
| 冻存条件 | 冻存液：55% 基础培养基+40%FBS+5%DMSO 温度：液氮 |
| 培养方案A（默认） |
生长培养基:
培养条件:
气相：空气，100%, 温度：37℃
|
| 培养方案B（可选） |
培养条件:
气相：空气，95%；CO2，5%, 温度：37℃
|
| 推荐传代比例 | 1:2-1:4 |
| 推荐换液频率 | 2-3次/周 |
| 注意事项 | 1. 该细胞推荐使用Leibovitz's L-15培养基进行培养，Leibovitz's L-15不可以通入二氧化碳，会产生细胞毒性； 2. 该细胞不建议更换为DMEM培养，圆形较多，效果不好。 |
| 背景描述 | SW480 [SW-480]细胞源自原位直肠腺癌，和SW620细胞源自同一病人一年后的淋巴结转移。CSAp和直肠抗体3阴性；角蛋白免疫过氧化物酶染色阳性。p53基因第273位密码子的G→A突变引起Arg→His替代，309位密码子的C→T突变导致Pro→Ser替代。细胞p53蛋白表达水平提高，癌基因c-myc、K-ras、H-ras、N-ras、myb、sis和fos的表达呈阳性，癌基因N-myc的表达未做检测。SW480 [SW-480]细胞不表达细胞溶解酶，一种与肿瘤入侵相关的金属蛋白酶。有报道称，SW480 [SW-480]细胞表达GM-CSF。SW480 [SW-480]细胞ras原癌基因的12位密码子有一个突变，可以用作PCR法检测该突变的阳性对照。1978年11月，A·Leibovitz将其提交给ATCC时已传代至第91代。 |
| 年龄（性别） | 男性；50岁 |
| 组织来源 | 直肠；结直肠腺癌 |
| 细胞类型 | 肿瘤细胞 |
| 肿瘤类型 | 肠癌细胞 |
| 生物安全等级 | BSL-1 |
| 倍增时间 | ~30-50 hours |
| 致瘤性 | Yes. Tumors developed within 21 days at 100% frequency (5/5) in nude mice inoculated subcutaneously with 1×10^7 cells. |
| 受体表达情况 | epidermal growth factor (EGF) |
| 基因表达情况 | carcinoembryonic antigen (CEA) 0.7 ng/10^6 cells/10 days; keratin; transforming growth factor beta, myc+; myb+ ; ras+; fos+; sis+; p53+; abl -; ros -; src -, HLA A2, B8, B17; Blood Type A; Rh+, The cells are positive for keratin by immunoperoxidase staining., The line is positive for expression of c-myc, K-ras, H-ras, N-ras, myb, sis and fos oncogenes. |
| 保藏机构 | ATCC; CCL-228 DSMZ; ACC-313 ECACC; 87092801 |
STR鉴定
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STR位点信息
Amelogenin X CSF1PO 13,14 D2S1338 17,24 D3S1358 15 D5S818 13 D7S820 8 D8S1179 13 D13S317 12 D16S539 13 D18S51 13 D19S433 13 D21S11 30,30.2 FGA 24 PentaD 9,15 PentaE 10 TH01 8 TPOX 11 vWA 16 D6S1043 11,12 D12S391 17 D2S441 10,15 -
STR鉴定图
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参考文献
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D3S-001, a KRAS G12C inhibitor with rapid target engagement kinetics, overcomes nucleotide cycling and demonstrates robust preclinical and clinical activities(2024/05/08)
作者：Jing Zhang, Sunmin Lim, Mira Yu
期刊：Cancer Discovery
DOI：10.1158/2159-8290.CD-24-0006
影响因子 ：28.200
引用产品：CL-0223
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Mitochondrial genome transfer drives metabolic reprogramming in adjacent colonic epithelial cells promoting TGFβ1-mediated tumor progression(2024/04/30)
作者：Bingjie Guan, Youdong Liu, Bowen Xie
期刊：Nature Communications
DOI：10.1038/s41467-024-48100-y
影响因子 ：16.600
引用产品：CL-0223
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CHIP-mediated ubiquitination of Galectin-1 predicts colorectal cancer prognosis(2020/01/14)
作者：Weimin Wang, Zhen Zhou, Liangliang Xiang
期刊：International Journal of Biological Sciences
影响因子 ：9.200
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Mitochondrial DNA content reduction induces aerobic glycolysis and reversible resistance to drug-induced apoptosis in SW480 colorectal cancer cells(2018/04/24)
作者：Jingjing Mou, Jin Peng, Yingying Shi
期刊：Biomedicine & Pharmacotherapy
DOI：10.1016/j.biopha.2018.04.099
影响因子 ：7.500
引用产品：CL-0223
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Discovery of a Potent, Cooperative, and Selective SOS1 PROTAC ZZ151 with In Vivo Antitumor Efficacy in KRAS-Mutant Cancers(2023/03/10)
作者：Zehui Zhou, Guizhen Zhou, Chuan Zhou
期刊：JOURNAL OF MEDICINAL CHEMISTRY
DOI：10.1021/acs.jmedchem.3c00075
影响因子 ：7.300
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A 3-circular RNA signature as a noninvasive biomarker for diagnosis of colorectal cancer(2019/11/04)
作者：Daoxiong Ye, Sisi Wang, Ying Huang
期刊：Cancer Cell International
影响因子 ：5.800
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LncRNA-ENST00000543604 exerts a tumor-promoting effect via miRNA 564/AEG-1 or ZNF326/EMT and predicts the prognosis of and chemotherapeutic effect in colorectal cancer.(2022/08/23)
作者：Weimin Wang, Zhen Zhou, Xiaojun Dai
期刊：Frontiers in Oncology
影响因子 ：5.738
引用产品：CL-0223
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SERPINB5 promotes colorectal cancer invasion and migration by promoting EMT and angiogenesis via the TNF-α/NF-κB pathway(2024/03/08)
作者：Bixia Liu, Yang Xie, Jiayu Zhang
期刊：INTERNATIONAL IMMUNOPHARMACOLOGY
DOI：10.1016/j.intimp.2024.111759
影响因子 ：5.600
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Circ_0011385 knockdown inhibits cell proliferation, migration and invasion, whereas promotes cell apoptosis by regulating miR-330-3p/MYO6 axis in colorectal cancer(2022/01/26)
作者：Jing Wang, Shaobo Ke, Yi Gong
期刊：Biomedical Journal
影响因子 ：5.500
引用产品：164210 164210-50 CL-0096 CL-0144 CL-0223 CL-0225 PM150110 PM150210
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Raltitrexed as a synergistic hyperthermia chemotherapy drug screened in patient-derived colorectal cancer organoids(2021/8/15)
作者：Lisi Zeng, Quanxing Liao, Haoran Zhao
期刊：Cancer Biology & Medicine
DOI：10.20892/j.issn.2095-3941.2020.0566
影响因子 ：5.500
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m6A methyltransferase METTL16 mediates immune evasion of colorectal cancer cells via epigenetically regulating PD-L1 expression(2023/08/29)
作者：Ailei Wang, Yingjie Sun, Xince Wang
期刊：Aging-US
影响因子 ：5.200
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Lycorine Induces autophagy-associated apoptosis by targeting MEK2 and enhances vemurafenib activity in colorectal cancer(2020/01/03)
作者：Man Hu, Zhaomin Yu, Peiyuan Mei
期刊：Aging-US
影响因子 ：5.200
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MicroRNA-934 facilitates cell proliferation, migration, invasion and angiogenesis in colorectal cancer by targeting B-cell translocation gene 2(2021/10/16)
作者：Bo Li, Xianyi Liu, Guogang Wu
期刊：Bioengineered
DOI：10.1080/21655979.2021.1996505.
影响因子 ：4.900
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RUNX1-induced upregulation of PTGS2 enhances cell growth, migration and invasion in colorectal cancer cells(2024/05/22)
作者：Mengchi Chen, Jingfeng Zhang, Hongyuan Huang
期刊：Scientific Reports
DOI：10.1038/s41598-024-60296-z
影响因子 ：4.600
引用产品：CL-0223
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Isoliquiritigenin Inhibits the Growth of Colorectal Cancer Cells through the ESR2/PI3K/AKT Signalling Pathway(2023/12/27)
作者：Fenglin Luo, Yimeng Tang, Lin Zheng
期刊：Pharmaceuticals
影响因子 ：4.600
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Hsa_circ_0003602 Contributes to the Progression of Colorectal Cancer by Mediating the miR-149-5p/SLC38A1 Axis(2022/09/23)
作者：Rong Wu, Shiyu Tang, Qiuxiao Wang
期刊：Gut and Liver
影响因子 ：4.321
引用产品：CL-0223
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Hsa-miR-425-5p promotes tumor growth and metastasis by activating the CTNND1-mediated β-catenin pathway and EMT in colorectal cancer(2020/06/28)
作者：Dingsheng Liu, Hong Zhang, Mingming Cui
期刊：Cell Cycle
DOI：10.1080/15384101.2020.1783058
影响因子 ：4.300
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Oct4 promoted proliferation, migration, invasion, and epithelial-mesenchymal transition (EMT) in colon cancer cells by activating the SCF/c-Kit signaling pathway(2022/10/18)
作者：Xiaoqian Bu, Yulong Liu, Lin Wang
期刊：CELL CYCLE
DOI：10.1080/15384101.2022.2112486
影响因子 ：4.300
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Circ_0056618 enhances PRRG4 expression by competitively binding to miR-411-5p to promote the malignant progression of colorectal cancer(2022/08/02)
作者：Bo Zhang, Wenbin Cao, Yang Liu
期刊：MOLECULAR AND CELLULAR BIOCHEMISTRY
DOI：10.1007/s11010-022-04525-x
影响因子 ：4.300
引用产品：164210 164210-50 CL-0096 CL-0144 CL-0223 CL-0225 PM150710 PM151010
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MicroRNA‑129 inhibits colorectal cancer cell proliferation, invasion and epithelial‑to‑mesenchymal transition by targeting SOX4(2021/03/24)
作者：Zhiping Chen, Tianyu Zhong, Jinghua Zhong
期刊：Oncol Rep
影响因子 ：4.136
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Deoxycholic acid-modified microporous SiO2 nanofibers mimicking colorectal microenvironment to optimize radiotherapy-chemotherapy combined therapy(2021/10/12)
作者：Lihuan Wang, Congzhao Zhao, Haoru Shan
期刊：Biomedical Materials
影响因子 ：4.103
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miR-145-5p restrained cell growth, invasion, migration and tumorigenesis via modulating RHBDD1 in colorectal cancer via the EGFR-associated signaling pathway(2019/11/03)
作者：Ying Niu, Jinping Zhang, Yalin Tong
期刊：International Journal Of Biochemistry & Cell Biology
DOI：10.1016/j.biocel.2019.105641
影响因子 ：4.000
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Intestinal metabolite xylulose inhibits colorectal cancer by inducing apoptosis through the MAPK signalling pathway(2024/05/10)
作者：Qing Hu, Qiao Zheng, Xinhao Du
期刊：TOXICOLOGY AND APPLIED PHARMACOLOGY
DOI：10.1016/j.taap.2024.116960
影响因子 ：3.800
引用产品：CL-0223
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NSUN6 mediates 5-methylcytosine modification of METTL3 and promotes colon adenocarcinoma progression(2024/05/27)
作者：Yuanbo Cui, Pengju Lv, Chunyan Zhang
期刊：JOURNAL OF BIOCHEMICAL AND MOLECULAR TOXICOLOGY
影响因子 ：3.600
引用产品：CL-0223
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HSPA5 repressed ferroptosis to promote colorectal cancer development by maintaining GPX4 stability(2022/09/01)
作者：Ronglin Wang, Lei Hua, Peixiang Ma
期刊：NEOPLASMA
DOI：10.4149/neo_2022_220331N363
影响因子 ：3.409
引用产品：CL-0223
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The bromodomain and extra-terminal domain inhibitor JQ1 synergistically sensitizes human colorectal cancer cells to topoisomerase I inhibitors through repression of Mre11-mediated DNA repair pathway(2020/09/26)
作者：Linping Lei, Xuqin Xie, Long He
期刊：Investigational New Drugs
DOI：10.1007/s10637-020-01014-0
影响因子 ：3.400
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Photodynamic effect of chlorin e6 on cytoskeleton protein of human colon cancer SW480 cells(2021/01/30)
作者：Haixiu Ma, Kaizhen Yang, Hongxia Li
期刊：Photodiagnosis and Photodynamic Therapy
DOI：10.1016/j.pdpdt.2021.102201
影响因子 ：3.300
引用产品：CL-0223
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Circ_0029803 serves as the sponge of miR-216b-5p to promote the progression of colorectal cancer by regulating SKIL expression(2021/09/03)
作者：Linfei Huang , Lei Zhu , Sheng Pan
期刊：World Journal of Surgical Oncology
DOI：10.1186/s12957-021-02368-2
影响因子 ：3.200
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SPNS2 promotes the malignancy of colorectal cancer cells via regulating Akt and ERK pathway(2019/6/30)
作者：Xinyue Gu, Yang Jiang, Weinan Xue
期刊：Clinical And Experimental Pharmacology And Physiology
影响因子 ：2.900
引用产品：CL-0223
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NDRG4 sensitizes CRC cells to 5-FU by upregulating DDIT3 expression(2021/9/13)
作者：Ruikai Li, Chenxiang He, Liangliang Shen
期刊：Oncology Letters
影响因子 ：2.900
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microRNA-4429-5p suppresses the malignant development of colon cancer by targeting matrix metalloproteinase 16(2021/08/26)
作者：Wei Li, Zhe Song, Nan Jia
期刊：In Vitro Cellular & Developmental Biology-Animal
DOI：10.1007/s11626-021-00603-4
影响因子 ：2.723
引用产品：CL-0050 CL-0096 CL-0144 CL-0223 CL-0225 PM150710 PM150910 PM151010
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circASS1 overexpression inhibits the proliferation, invasion and migration of colorectal cancer cells by regulating the miR‑1269a/VASH1 axis(2021/08/10)
作者：HaiLin Xiong, XiaoHua Zhong, XiaoHong Guo
期刊：Experimental and Therapeutic Medicine
影响因子 ：2.700
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Exosomes-derived miR-548am-5p promotes colorectal cancer progression(2023/11/30)
作者：Fangfang Li, Minglei Zhang, Xiaodong Yin
期刊：CELLULAR AND MOLECULAR BIOLOGY
DOI：10.14715/cmb/2023.69.12.17
影响因子 ：1.600
引用产品：CL-0223
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The Upregulation of GSTO2 is Associated with Colon Cancer Progression and a Poor Prognosis(2023/01/11)
作者：Xingyu Peng, Jinfeng Zhu, Sicheng Liu
期刊：Journal of Oncology
影响因子 ：0.000
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