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名称 | SW480 [SW-480] (人结肠癌细胞) (STR鉴定正确) |
别称 | SW-480; SW 480; SW480E |
种属 | 人类 |
生长特性 | 贴壁细胞 |
细胞形态 | 上皮细胞样 |
冻存条件 | 冻存液:55% 基础培养基+40%FBS+5%DMSO 温度:液氮 |
培养方案A(默认) |
生长培养基:
培养条件:
气相:空气,100%, 温度:37℃
|
培养方案B(可选) |
培养条件:
气相:空气,95%;CO2,5%, 温度:37℃
|
推荐传代比例 | 1:2-1:4 |
推荐换液频率 | 2-3次/周 |
注意事项 | 1. 该细胞推荐使用Leibovitz's L-15培养基进行培养,Leibovitz's L-15不可以通入二氧化碳,会产生细胞毒性; 2. 该细胞不建议更换为DMEM培养,圆形较多,效果不好。 |
背景描述 | SW480 [SW-480]细胞源自原位直肠腺癌,和SW620细胞源自同一病人一年后的淋巴结转移。CSAp和直肠抗体3阴性;角蛋白免疫过氧化物酶染色阳性。p53基因第273位密码子的G→A突变引起Arg→His替代,309位密码子的C→T突变导致Pro→Ser替代。细胞p53蛋白表达水平提高,癌基因c-myc、K-ras、H-ras、N-ras、myb、sis和fos的表达呈阳性,癌基因N-myc的表达未做检测。SW480 [SW-480]细胞不表达细胞溶解酶,一种与肿瘤入侵相关的金属蛋白酶。有报道称,SW480 [SW-480]细胞表达GM-CSF。SW480 [SW-480]细胞ras原癌基因的12位密码子有一个突变,可以用作PCR法检测该突变的阳性对照。1978年11月,A·Leibovitz将其提交给ATCC时已传代至第91代。 |
年龄(性别) | 男性;50岁 |
组织来源 | 直肠;结直肠腺癌 |
细胞类型 | 肿瘤细胞 |
肿瘤类型 | 肠癌细胞 |
生物安全等级 | BSL-1 |
倍增时间 | ~30-50 hours |
致瘤性 | Yes. Tumors developed within 21 days at 100% frequency (5/5) in nude mice inoculated subcutaneously with 1×10^7 cells. |
受体表达情况 | epidermal growth factor (EGF) |
基因表达情况 | carcinoembryonic antigen (CEA) 0.7 ng/10^6 cells/10 days; keratin; transforming growth factor beta, myc+; myb+ ; ras+; fos+; sis+; p53+; abl -; ros -; src -, HLA A2, B8, B17; Blood Type A; Rh+, The cells are positive for keratin by immunoperoxidase staining., The line is positive for expression of c-myc, K-ras, H-ras, N-ras, myb, sis and fos oncogenes. |
保藏机构 | ATCC; CCL-228 DSMZ; ACC-313 ECACC; 87092801 |
STR鉴定
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STR位点信息
Amelogenin X CSF1PO 13,14 D2S1338 17,24 D3S1358 15 D5S818 13 D7S820 8 D8S1179 13 D13S317 12 D16S539 13 D18S51 13 D19S433 13 D21S11 30,30.2 FGA 24 PentaD 9,15 PentaE 10 TH01 8 TPOX 11 vWA 16 D6S1043 11,12 D12S391 17 D2S441 10,15 -
STR鉴定图
参考文献
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D3S-001, a KRAS G12C inhibitor with rapid target engagement kinetics, overcomes nucleotide cycling and demonstrates robust preclinical and clinical activities(2024/05/08)
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DOI:10.1016/j.biopha.2018.04.099
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DOI:10.1080/21655979.2021.1996505.
影响因子 :4.900
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DOI:10.1038/s41598-024-60296-z
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