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产品概述
| 名称 | A549/DDP (人肺腺癌耐顺铂株)(STR鉴定正确) |
| 种属 | 人 |
| 生长特性 | 贴壁细胞 |
| 细胞形态 | 上皮细胞样 |
| 冻存条件 | 冻存液：55% 基础培养基+40%FBS+5%DMSO 温度：液氮 |
| 培养方案A（默认） |
生长培养基:
培养条件:
气相：空气，95%；CO2，5%, 温度：37℃
|
| 推荐传代比例 | 1:2-1:3 |
| 推荐换液频率 | 2-3次/周 |
| 注意事项 | 耐药株生长速度慢，1:2传代一周左右可以再次传代，细胞形态比较梭长。当细胞生长速度过慢时，可以采用以下方式进行扩增培养：用含低浓度药物的培养基连续培养2周，期间根据细胞密度，需要传代时，传代处理。待细胞状态恢复后，提高培养基中药物的浓度进行培养，此时梯度加药，每个浓度培养一个代次（即持续培养到长满传代，即换下一个浓度），之后细胞状态没有问题的话，持续用加药培养基进行培养即可。 |
| 细胞类型 | 肿瘤细胞 |
| 肿瘤类型 | 肺癌细胞 |
| 生物安全等级 | BSL-1 |
STR鉴定
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STR位点信息
Amelogenin X,Y CSF1PO 10,12 D2S1338 24 D3S1358 16 D5S818 11 D7S820 8,11 D8S1179 13,14 D13S317 11 D16S539 11,12 D18S51 14,17 D19S433 13 D21S11 29 FGA 23 PentaD 9 PentaE 7,11 TH01 8,9.3 TPOX 8,11 vWA 14 D6S1043 11,13 D12S391 18 D2S441 10,13 -
STR鉴定图
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参考文献
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Hypoxia-triggered tumor specific glutamine inhibition for reversing cisplatin resistance of chemotherapy(2023/11/27)
作者：Jiaying Zhang, Jinghui Hu, Weiwei Zhu
期刊：CHEMICAL ENGINEERING JOURNAL
影响因子 ：15.100
引用产品：CL-0519
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Inhibition of autophagy potentiates the cytotoxicity of the irreversible FGFR1-4 inhibitor FIIN-2 on lung adenocarcinoma(2022/08/30)
作者：Xiuqin Jia, Ming Xin, Juanjuan Xu
期刊：Cell Death & Disease
DOI：10.1038/s41419-022-05201-0
影响因子 ：9.000
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Self-Assembly of Erlotinib-Platinum(II) Complexes for Epidermal Growth Factor Receptor-Targeted Photodynamic Therapy(2024/01/06)
作者：Haobing Wang, Yidan Lai, Dan Li
期刊：JOURNAL OF MEDICINAL CHEMISTRY
DOI：10.1021/acs.jmedchem.3c01889
影响因子 ：7.300
引用产品：CL-0519
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A Clinically Translatable Ternary Platinum(IV) Prodrug for Synergistically Reversing Drug Resistance(2023/03/10)
作者：Ying Liu, Dun Wang, Hongbing Liu, Li Liu
期刊：JOURNAL OF MEDICINAL CHEMISTRY
DOI：10.1021/acs.jmedchem.2c01924
影响因子 ：7.300
引用产品：CL-0519
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CXCR5 guides migration and tumor eradication of anti-EGFR chimeric antigen receptor T cells(2021/07/16)
作者：Guangchao Li, Jintao Guo, Yanfang Zheng
期刊：Molecular Therapy-Oncolytics
DOI：10.1016/j.omto.2021.07.003
影响因子 ：5.700
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Targeting Polo-like Kinase 1 by a Novel Pyrrole-Imidazole Polyamide–Hoechst Conjugate Suppresses Tumor Growth In Vivo(2018/05/01)
作者：Ke Liu, Lijing Fang, Haiyan Sun
期刊：Molecular Cancer Therapeutics
DOI：10.1158/1535-7163.MCT-17-0747
影响因子 ：5.700
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Assessment of Mononuclear/Dinuclear copper acylhydrazone complexes for lung cancer treatment(2024/01/14)
作者：Youru Wu, Daqi Wu, Jianfeng Lan
期刊：BIOORGANIC CHEMISTRY
DOI：10.1016/j.bioorg.2024.107122
影响因子 ：5.100
引用产品：CL-0519
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A ratiometric near-infrared fluorescence strategy based on spiropyran in situ switching for tracking dynamic changes of live-cell lysosomal pH(2019/03/27)
作者：Jin Li, Xiaokang Li, Jianbo Jia
期刊：Dyes And Pigments
DOI：10.1016/j.dyepig.2019.03.060
影响因子 ：4.500
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A novel colorimetric and ratiometric fluorescent probe for selective detection of bisulfite in real samples and living cells(2018/12/07)
作者：Jin Li, Ying Gao, Haoran Guo
期刊：Dyes And Pigments
DOI：10.1016/j.dyepig.2018.12.009
影响因子 ：4.500
引用产品：CL-0519
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A cisplatin and disulfiram co-loaded inclusion complex overcomes drug resistance by inhibiting cancer cell stemness in non-small cell lung cancer(2023/12/22)
作者：Wenhui Ye, Huaiyou Lv, Qinxiu Zhang
期刊：JOURNAL OF DRUG TARGETING
DOI：10.1080/1061186X.2023.2298844
影响因子 ：4.500
引用产品：CL-0519
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Circ_0006988 promotes the proliferation, metastasis and angiogenesis of non-small cell lung cancer cells by modulating miR-491-5p/MAP3K3 axis(2021/06/30)
作者：Chao Yang, Jiang Shi, Jie Wang
期刊：Cell Cycle
DOI：10.1080/15384101.2021.1941612
影响因子 ：4.300
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Circ_0017956 promotes the proliferation and metastasis of non-small cell lung cancer through regulating miR-515-5p/ITGB8 axis(2021/05/04)
作者：Na Li, Dan Zhu
期刊：Cell Cycle
DOI：10.1080/15384101.2021.1919829
影响因子 ：4.300
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Mixed-ligand copper(II) hydrazone complexes: Synthesis, structure, and anti-lung cancer properties(2023/01/19)
作者：YouRu Wu, LiXia Hou, JianFeng Lan
期刊：JOURNAL OF MOLECULAR STRUCTURE
DOI：10.1016/j.molstruc.2023.134986
影响因子 ：3.800
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p32/OPA1 axis-mediated mitochondrial dynamics contributes to cisplatin resistance in non-small cell lung cancer: p32/OPA1 contributes to cisplatin resistance(2023/12/26)
作者：Chunxia Yu, Zheqing Peng, Tao Wang
期刊：ACTA BIOCHIMICA ET BIOPHYSICA SINICA
影响因子 ：3.700
引用产品：CL-0519
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Role and mechanism of COL3A1 in regulating the growth, metastasis, and drug sensitivity in cisplatin-resistant non-small cell lung cancer cells(2024/03/26)
作者：Jiankun Ren, Songwei Zhao, Junyu Lai
期刊：CANCER BIOLOGY & THERAPY
DOI：10.1080/15384047.2024.2328382
影响因子 ：3.600
引用产品：CL-0519
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Mechanism of PWAR6 regulating cisplatin drug sensitivity in non-small cell lung cancer through miR-577/PHACTR1(2023/11/03)
作者：Wenjun Tang, Jingru Luo, Shu Lin
期刊：GENE
DOI：10.1016/j.gene.2023.147954
影响因子 ：3.500
引用产品：CL-0519
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Demethoxycurcumin increases the sensitivity of cisplatin‑resistant non‑small lung cancer cells to cisplatin and induces apoptosis by activating the caspase signaling pathway(2020/9/8)
作者：Yun Chen, Chaojin Hong, Xiaochen Chen
期刊：Oncology Letters
影响因子 ：2.900
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Overexpression of close homolog of L1 enhances the chemosensitivity of lung cancer cells via inhibition of the Akt pathway(2020/08/12)
作者：Xiangdao Cai, Bang Hu, Sheng Liu
期刊：Oncology Letters
影响因子 ：2.900
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Andrographis modulates cisplatin resistance in lung cancer via miR-155-5p/SIRT1 axis(2023/08/02)
作者：Chong Pang, Tengyue Zhang, Yulong Chen
期刊：FUNCTIONAL & INTEGRATIVE GENOMICS
DOI：10.1007/s10142-023-01186-x
影响因子 ：2.900
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The impact of SOX4-activated CTHRC1 transcriptional activity regulating DNA damage repair on cisplatin resistance in lung adenocarcinoma(2024/04/17)
作者：Cheng Ai, Zhenhao Huang, Tenghao Rong
期刊：ELECTROPHORESIS
影响因子 ：2.900
引用产品：CL-0519
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Anlotinib Inhibits Cisplatin Resistance in Non-Small-Cell Lung Cancer Cells by Inhibiting MCL-1 Expression via MET/STAT3/Akt Pathway(2024/03/04)
作者：Lile Wang, Lu Xu, Shuhua Han
期刊：Canadian Respiratory Journal
影响因子 ：2.200
引用产品：CL-0519
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MicroRNA-106a-5p alleviated the resistance of cisplatin in lung cancer cells by targeting Jumonji domain containing 6(2021/10/02)
作者：Xiang Ge, Yifei Jiang, Xun Hu
期刊：Transplant Immunology
DOI：10.1016/j.trim.2021.101478
影响因子 ：2.032
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