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产品概述
名称 | MC3T3-E1 Subclone 14 (小鼠颅顶前骨细胞亚克隆14) (STR鉴定正确) |
别称 | MC3T3-E1 SUBCLONE 14 |
种属 | 小鼠 |
生长特性 | 贴壁细胞 |
细胞形态 | 成纤维细胞样 |
冻存条件 | 冻存液:55% 基础培养基+40%FBS+5%DMSO 温度:液氮 |
培养方案A(默认) |
培养条件:
气相:空气,95%;CO2,5%, 温度:37℃
|
推荐传代比例 | 1:3-1:4 |
推荐换液频率 | 2-3次/周 |
背景描述 | 从克隆的但是表型各异的MC3T3-E1细胞系中分离出一系列亚克隆,从含抗坏血酸培养基生长的成骨细胞中选择高或低成骨细胞分化、矿化的亚克隆。MC3T3-E1 Subclone 4和MC3T3-E1 Subclone 14在抗坏血酸和3-4mM无机磷酸盐中生长表现出高水平的成骨细胞分化。它们10天后形成一个矿化良好的细胞外基质(ECM)。MC3T3-E1 Subclone 24和MC3T3-E1 Subclone 30在抗坏血酸中生长表现出很差的成骨细胞分化,不形成ECM,可以作为MC3T3-E1 Subclone 4和MC3T3-E1 Subclone 14的阴性对照。矿化的亚克隆选择的表达作为成骨细胞标记的mRNA及唾液酸糖蛋白(BSP)、骨钙素(OCN)和甲状旁腺激素/甲状旁腺激素相关蛋白受体的mRNA。高或者低的分化潜能的亚克隆在培养中生产出相似数量的胶原质,表达可比较的基本水平的mRNA编码Osf2/Cbfa1,一种成骨细胞相关转录因子。植入免疫缺陷小鼠以后,高分化性的亚克隆形成与骨类似的形成小骨的编织骨,低分化细胞只是产生纤维组织。这些细胞系是研究体外成骨细胞分化的好模型,尤其是ECM信号。它们和原代培养颅顶成骨细胞的行为类似。 |
年龄(性别) | 新生 |
组织来源 | 颅顶骨 |
细胞类型 | 自发永生化细胞 |
生物安全等级 | BSL-1 |
致瘤性 | Yes, in immunodeficient mice (forms bone-like ossicles). |
基因表达情况 | collagen |
保藏机构 | ATCC; CRL-2594 |
STR鉴定
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STR位点信息
18-3 15 6-7 17 5-5 17 X-1 28 1-2 19 7-1 26.2 8-1 16 1-1 16,17 19-2 13 15-3 22.3 12-1 17 6-4 18 4-2 20.3 3-2 14 2-1 16 13-1 16.1
11-2 16 17-2 16 -
STR鉴定图
参考文献
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A clinical-stage Nrf2 activator suppresses osteoclast differentiation via the iron-ornithine axis(2024/04/02)
作者:Yimin Dong, Honglei Kang, Renpeng Peng
期刊:Cell Metabolism
DOI:10.1016/j.cmet.2024.03.005
影响因子 :29.000
引用产品:CL-0378
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A novel photothermally controlled multifunctional scaffold for clinical treatment of osteosarcoma and tissue regeneration(2020/06/01)
作者:Liang Ma, Xiaobo Feng, Hang Liang
期刊:Materials Today
DOI:10.1016/j.mattod.2019.12.005
影响因子 :24.200
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Local bone metabolism balance regulation via double-adhesive hydrogel for fixing orthopedic implants(2021/10/19)
作者:Wei Jiang, Fushan Hou, Yong Gu
期刊:Bioactive Materials
DOI:10.1016/j.bioactmat.2021.10.017
影响因子 :18.900
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A bone-targeted engineered exosome platform delivering siRNA to treat osteoporosis(2021/09/17)
作者:Yongzhi Cui, Yuanyuan Guo, Li Kong
期刊:Bioactive Materials
DOI:10.1016/j.bioactmat.2021.09.015
影响因子 :18.900
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An injectable self-adaptive polymer as a drug carrier for the treatment of nontraumatic early-stage osteonecrosis of the femoral head(2022/03/12)
作者:Ning Kong, Hang Yang, Run Tian
期刊:Bone Research
DOI:10.1038/s41413-022-00196-y
影响因子 :12.700
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An oxidized dextran-composite self-healing coated magnesium scaffold reduces apoptosis to induce bone regeneration(2023/12/07)
作者:Zewen Shi, Fang Yang, Yiwei Hu
期刊:CARBOHYDRATE POLYMERS
DOI:10.1016/j.carbpol.2023.121666
影响因子 :11.200
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Electron transfer correlated antibacterial activity of biocompatible graphene Nanosheets-TiO2 coatings(2020/05/22)
作者:Minggang Yang, Hui Liu, Caijie Qiu
期刊:Carbon
DOI:10.1016/j.carbon.2020.05.036
影响因子 :10.900
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Ångstrom-scale gold particles loaded with alendronate via alpha-lipoic acid alleviate bone loss in osteoporotic mice(2024/04/30)
作者:Weihang Gao, Jiaojiao Li, Jingyu Shi
期刊:JOURNAL OF NANOBIOTECHNOLOGY
DOI:10.1186/s12951-024-02466-9
影响因子 :10.200
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Title A horn Peptide-Thermoresponsive Hydrogel for Angiogenesis and Bone Regeneration(2024/03/29)
作者:Nannan Xue, Pei Liu, Wenjian Zhao
期刊:Advanced Healthcare Materials
影响因子 :10.000
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Bioinspired self-healing injectable nanocomposite hydrogels based on oxidized dextran and gelatin for growth-factor-free bone regeneration(2023/08/06)
作者:Wuchao Ma, Meilian Yang, Chao Wu, Shaoyun Wang, Ming Du
期刊:INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
DOI:10.1016/j.ijbiomac.2023.126145
影响因子 :8.200
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Surface functionalization of calcium magnesium phosphate cements with alginate sodium for enhanced bone regeneration via TRPM7/PI3K/Akt signaling pathway(2024/03/22)
作者:Changtian Gong, Jian Yang, Xiping Zhang
期刊:INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
DOI:10.1016/j.ijbiomac.2024.130998
影响因子 :8.200
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Inhibition of Nrf2 degradation alleviates age-related osteoporosis induced by 1,25-Dihydroxyvitamin D deficiency(2021/12/08)
作者:Renlei Yang, Jiao Zhang, Jie Li
期刊:Free Radical Biology And Medicine
DOI:10.1016/j.freeradbiomed.2021.12.010
影响因子 :8.101
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Novel Antiosteoporotic Peptides Purified from Protein Hydrolysates of Taihe black-boned silky fowl: By larval Zebrafish Model and Molecular docking(2023/04/19)
作者:Yang Qiu, Jianyue Ying, Fujie Yan
期刊:FOOD RESEARCH INTERNATIONAL
DOI:10.1016/j.foodres.2023.112850
影响因子 :8.100
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The osteogenesis and the biologic mechanism of thermo-responsive injectable hydrogel containing carboxymethyl chitosan/sodium alginate nanoparticles towards promoting osteal wound healing(2022/10/18)
作者:Zewen Shi, Fang Yang, Qian Pang
期刊:INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
DOI:10.1016/j.ijbiomac.2022.10.142
影响因子 :8.025
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Fabrication of Submicro-Nano Structures on Polyetheretherketone Surface by Femtosecond Laser for Exciting Cellular Responses of MC3T3-E1 Cells/Gingival Epithelial Cells(2023/03/17)
作者:Dong Xie, Chenhui Xu, Cheng Ye
期刊:International Journal of Nanomedicine
影响因子 :8.000
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High Strength and Shape Memory Spinal Fusion Device for Minimally Invasive Interbody Fusions(2024/05/31)
作者:Min Liu, Bo Liu, Ziyang Liu
期刊:International Journal of Nanomedicine
影响因子 :8.000
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Effects of the combination of Epimedii Folium and Ligustri Lucidi Fructus on apoptosis and autophagy in SOP rats and osteoblasts via PI3K/AKT/mTOR pathway(2024/03/01)
作者:Yuman Li, Ping Yu, Yingying Gao
期刊:BIOMEDICINE & PHARMACOTHERAPY
DOI:10.1016/j.biopha.2024.116346
影响因子 :7.500
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Activation of TGR5 promotes osteoblastic cell differentiation and mineralization(2018/10/16)
作者:Qingfeng Wang, Guoqiang Wang, Bin Wang
期刊:Biomedicine & Pharmacotherapy
DOI:10.1016/j.biopha.2018.08.093
影响因子 :7.500
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Corylifol A protects against ovariectomized-induced bone loss and attenuates RANKL‐induced osteoclastogenesis via ROS reduction, ERK inhibition, and NFATc1 activation(2023/01/14)
作者:Yinglong Xu, Dezhi Song, Xixi Lin
期刊:FREE RADICAL BIOLOGY AND MEDICINE
DOI:10.1016/j.freeradbiomed.2023.01.017
影响因子 :7.400
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Amorphous Calcium Magnesium Phosphate Nanocomposites with Superior Osteogenic Activity for Bone Regeneration(2021/11/24)
作者:Jiang Yingying, Tan Shuo, Hu Jianping
期刊:Regenerative Biomaterials
影响因子 :6.700
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Osteo-angiogenic activity of a micro/nano hierarchical SrSi-codoped hydroxyapatite coating on zirconium alloy(2024/05/13)
作者:Xiaoning Xu, Kaige Wang, Xinrong Tan
期刊:Journal of Materials Research and Technology-JMR&T
DOI:10.1016/j.jmrt.2024.05.067
影响因子 :6.400
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Covalent immobilization of VEGF on allogeneic bone through polydopamine coating to improve bone regeneration(2022/10/12)
作者:Jianhao Huang, Jingwei Lu, Ziying Liu
期刊:Frontiers in Bioengineering and Biotechnology
DOI:10.3389/fbioe.2022.1003677
影响因子 :6.064
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Canagliflozin promotes osteoblastic MC3T3-E1 differentiation via AMPK/RUNX2 and improves bone microarchitecture in type 2 diabetic mice(2022/12/16)
作者:Peiyang Song, Tianyi Chen, Shunli Rui
期刊:Frontiers in Endocrinology
DOI:10.3389/fendo.2022.1081039
影响因子 :6.055
引用产品:CL-0378
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3D-Printed Scaffolds of Porous Amorphous Calcium Phosphate Nanospheres Loaded with Quercetin for Promoting Bone Repair via Synergistic Osteogenesis and Immunoregulation(2024/04/24)
作者:Rui Xu, Jie Yin, Lan Li
期刊:ACS Applied Nano Materials
影响因子 :5.900
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Bilayer Membrane Composed of Mineralized Collagen and Chitosan Cast Film Coated With Berberine-Loaded PCL/PVP Electrospun Nanofiber Promotes Bone Regeneration(2021/7/19)
作者:Yuhan Zhang, Ting Wang, Juan Li
期刊:Frontiers in Bioengineering and Biotechnology
影响因子 :5.700
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IGF2BP2, an RNA-binding protein regulates cell proliferation and osteogenic differentiation by stabilizing SRF mRNA(2022/11/27)
作者:Zimo Zhou, Senxiang Chen, Tong Wu, Yifeng Chen, Yuxiao Cao, Ying Huang, Da Liu
期刊:JOURNAL OF CELLULAR PHYSIOLOGY
影响因子 :5.600
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Dipeptidyl peptidase 3 is essential for maintaining osteoblastic differentiation under a high-glucose environment by inhibiting apoptosis, oxidative stress and inflammation through the modulation of the Keap1-Nrf2 pathway(2023/06/03)
作者:Xiaofeng Li, Xiangyou Yu, Shenglin He
期刊:INTERNATIONAL IMMUNOPHARMACOLOGY
DOI:10.1016/j.intimp.2023.110404
影响因子 :5.600
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Er-xian decoction drug-containing serum promotes Mc3t3-e1 cell proliferation and osteogenic differentiation via regulating BK channel(2022/11/01)
作者:Ming-Shi Ren, Hui-Hui Xie, Yu Ding, Zi-Han Li, Bo Liu
期刊:JOURNAL OF ETHNOPHARMACOLOGY
影响因子 :5.400
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Apocynin exerts cytoprotective effects on dexamethasone-induced osteoblasts by inhibiting oxidative stress through the Nrf2 signalling pathway(2023/09/25)
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The effect of calcitonin gene-related peptide functionalized TiO2 nanotubes on osteoblast and osteoclast differentiation in vitro(2020/05/05)
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Effects of a novel self-assembling peptide scaffold on bone regeneration and controlled release of two growth factors(2021/12/07)
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Study on complications of osteoporosis based on network pharmacology(2022/09/29)
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Study of Injectable Hydrogel Based on ALN/Nha Promoting Osteogenesis And Inhibiting Osteoclasts in Osteoporotic Bone Defects Repair(2024/01/12)
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期刊:MACROMOLECULAR BIOSCIENCE
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A novel fluorescent probe for detecting hydrogen sulfide in osteoblasts during lipopolysaccharide-mediated inflammation under periodontitis(2021/10/11)
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Resveratrol Protects Osteoblasts Against Dexamethasone-Induced Cytotoxicity Through Activation of AMP-Activated Protein Kinase(2022/10/31)
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Elucidating the role of the GC/GR/GLUT1 axis in steroid-induced osteonecrosis of the femoral head: A proteomic approach(2024/03/20)
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Hydroxyapatite formation in biomimetic synthesis with the interface of a pDA@SIS membrane(2022/05/03)
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期刊:RSC Advances
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Microstructured titanium functionalized by naringin inserted multilayers for promoting osteogenesis and inhibiting osteoclastogenesis(2021/08/10)
作者:Ke Shen, Xiaojing Zhang, Qiang Tang
期刊:Journal Of Biomaterials Science-Polymer Edition
DOI:10.1080/09205063.2021.1949098
影响因子 :3.600
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D-RADA16-RGD-Reinforced Nano-Hydroxyapatite/Polyamide 66 Ternary Biomaterial for Bone Formation(2019/01/05)
作者:WeiKang Zhao, Bin He, Ao Zhou
期刊:Tissue Engineering and Regenerative Medicine
影响因子 :3.600
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Polydopamine-mediated immobilization of BMP-2 onto electrospun nanofibers enhances bone regeneration(2024/04/30)
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Comprehensive reparative effects of bacteriostatic poly(L-lactide-co-glycolide)/poly(L-lactide-co-ε-caprolactone) electrospinning membrane on alveolar bone defects in progressive periodontitis(2022/10/05)
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期刊:JOURNAL OF BIOMEDICAL MATERIALS RESEARCH PART B-APPLIED BIOMATERIALS
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Adhesion and Proliferation of Osteoblast-Like Cells on Porous Polyetherimide Scaffolds(2018/11/27)
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期刊:Biomed Research International
影响因子 :3.246
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The initial application of conch-shell hydroxyapatite and HACC as functional filler in PMMA bone cement(2023/08/06)
作者:Luqi Tian, Ronge Xing, Haoyue Yang
期刊:International Journal of Polymeric Materials and Polymeric Biomaterials
DOI:10.1080/00914037.2023.2243367
影响因子 :3.200
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Fabrication of porous gehlenite coating on Al2O3–ZrO2–SiC composite ceramics and its in vitro biological activities:(2022/03/24)
作者:Xianzhi Chen, Jiaxin Li, Shuidan Gu
期刊:Journal Of Biomaterials Applications
影响因子 :2.900
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Unraveling IGFBP3-mediated m6A modification in fracture healing(2024/02/22)
作者:Aining Lai, Junjian Sun, Zhiyuan Dai
期刊:PATHOLOGY RESEARCH AND PRACTICE
影响因子 :2.800
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p-Smad3 differentially regulates the cytological behavior of osteoclasts before and after osteoblasts maturation(2024/04/17)
作者:Jiapeng Ye, Zhen Hua, Jirimutu Xiao
期刊:MOLECULAR BIOLOGY REPORTS
DOI:10.1007/s11033-024-09400-0
影响因子 :2.800
引用产品:CL-0378
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Irisin enhances osteogenic differentiation of mouse MC3T3‑E1 cells via upregulating osteogenic genes(2021/5/31)
作者:Jian Yang, Kai Yu, Dongmei Liu
期刊:Experimental and Therapeutic Medicine
影响因子 :2.700
引用产品:CL-0378
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FNDC5/irisin ameliorates bone loss of type 1 diabetes by suppressing endoplasmic reticulum stress‑mediated ferroptosis(2024/03/30)
作者:Qianqian Dong, Ziqi Han, Mingdong Gao
期刊:Journal of Orthopaedic Surgery and Research
DOI:10.1186/s13018-024-04701-3
影响因子 :2.600
引用产品:CL-0378
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MiR-708 inhibits MC3T3-E1 cells against H2O2-induced apoptosis through targeting PTEN(2020/7/10)
作者:Wei Zhang, Shengyu Cui, Hong Yi
期刊:Journal of Orthopaedic Surgery and Research
DOI:10.1186/s13018-020-01780-w
影响因子 :2.600
引用产品:CL-0378
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Composition and bioactivity of calcium phosphate coatings on anodic oxide nanotubes formed on pure Ti and Ti-6Al-4V alloy substrates(2020/01/23)
作者:Qianting Yao, Yingying Jiang, Shuo Tan
期刊:Materials Science and Engineering: C
DOI:10.1016/j.msec.2020.110687
影响因子 :1.900
引用产品:CL-0378
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Biomimetic Synthesis and Mechanism of Hydroxyapatite Crystals by Biotemplate of Small Intestinal Submucosa and Bacterial Cellulose(2023/06/25)
作者:Qiuhong Zhu, Hua Jiao, Kang Zhao
期刊:CRYSTAL RESEARCH AND TECHNOLOGY
影响因子 :1.500
引用产品:CL-0378
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Positive space acquiring asymmetric membranes for guiding alveolar bone regeneration under infectious conditions(2022/12/17)
作者:Bing Wang, Chuanlan Qin, Yiming Liu
期刊:Biomaterials Advances
DOI:10.1016/j.bioadv.2022.213252
影响因子 :0.000
引用产品:CL-0378
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