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| 名称 | DLD-1 (人结直肠腺癌上皮细胞) (STR鉴定正确) |
| 别称 | DLD 1; DLD1; CoCL3 |
| 种属 | 人 |
| 生长特性 | 贴壁细胞 |
| 细胞形态 | 上皮细胞样 |
| 冻存条件 | 冻存液：55% 基础培养基+40%FBS+5%DMSO 温度：液氮 |
| 培养方案A（默认） |
培养条件:
气相：空气，95%；CO2，5%, 温度：37℃
|
| 推荐传代比例 | 1:3-1:4 |
| 推荐换液频率 | 2-3次/周 |
| 背景描述 | DLD-1细胞是由D·L·Dexter和其同事于1977-1979年分离的两株结直肠腺癌细胞株中的一株。在ATCC和其它地方进行的DNA指纹鉴定和染色体组型分析表明DLD-1细胞与HCT-15细胞相似，说明这两者是来自同一个人的不同克隆。DLD-1细胞和HCT-15细胞的遗传起源可通过DNA指纹鉴定证实，但染色体组型分析显示它们缺乏染色体标记一致改变或数目上一致改变。DLD-1细胞的CSAp阴性(CSAp-)，p53抗原表达呈阳性(p53抗原产生了一个C→T点突变导致241位的Ser→Phe)。DLD-1细胞角蛋白免疫过氧化物酶染色阳性，癌基因c-myc、K-ras、H-ras、N-ras、myb、sis和fos的表达呈阳性，癌基因N-myc的表达未做检测。DLD-1细胞表达肿瘤特异性核基质蛋白CC-2、CC-3、CC-4、CC-5和CC-6。1979年提交到ATCC的DLD-1细胞代数不明且污染了支原体，其后经过12周多种抗生素联合培养处理，处理之后每周用Hoechst染色和标准培养法检测。其后连续11个月不加抗生素培养，DLD-1细胞所有的检测呈阴性。 |
| 年龄（性别） | 男性；成人 |
| 组织来源 | 结直肠腺癌上皮细胞 |
| 细胞类型 | 肿瘤细胞 |
| 肿瘤类型 | 肠癌细胞 |
| 生物安全等级 | BSL-1 |
| 倍增时间 | ~24-48 hours |
| 致瘤性 | Yes, in nude mice (Tumors developed within 21 days at 100% frequency (5/5) in nude mice inoculated subcutaneously with 1×10^7 cells). |
| 抗原表达情况 | Blood Type O.The cells are weakly positive for keratins and vimentin.The cells are positive for keratin by immunoperoxidase staining.DLD-1 cells are positive for p53 antigen expression (the p53 antigen produced has a C -> T |
| 基因表达情况 | carcinoembryonic antigen (CEA) 0.5 ng/10^6 cells/10 days; colon antigen 3. |
| 保藏机构 | ATCC; CCL-221 BCRC; 60132 DSMZ; ACC-278 ECACC; 90102540 |
STR鉴定
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STR位点信息
Amelogenin X,Y CSF1PO 11,12 D2S1338 17,25 D3S1358 17 D5S818 13 D7S820 10,12 D8S1179 15 D13S317 8,11 D16S539 12,13 D18S51 11,17 D19S433 14,16 D21S11 29,32.2 FGA 22 PentaD 9,14 PentaE 7,14 TH01 7,9.3 TPOX 8,11 vWA 18,19 D6S1043 11,13 D12S391 19,22 D2S441 13,15 -
STR鉴定图
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参考文献
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Blockade of the deubiquitinating enzyme USP48 degrades oncogenic HMGA2 and inhibits colorectal cancer invasion and metastasis(2024/01/17)
作者：Can Cheng, Hanhui Yao, Heng Li
期刊：Acta Pharmaceutica Sinica B
DOI：10.1016/j.apsb.2024.01.006
影响因子 ：14.500
引用产品：CL-0074
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Acetyltransferase NAT10 regulates the Wnt/β-catenin signaling pathway to promote colorectal cancer progression via ac4C acetylation of KIF23 mRNA(2022/12/15)
作者：Chi Jin, Tuo Wang, Dongsheng Zhang
期刊：JOURNAL OF EXPERIMENTAL & CLINICAL CANCER RESEARCH
DOI：10.1186/s13046-022-02551-7
影响因子 ：11.300
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Ubiquitination-mediated degradation of SIRT1 by SMURF2 suppresses CRC cell proliferation and tumorigenesis(2020/05/02)
作者：Le Yu, Ling Dong, Hui Li
期刊：Oncogene
影响因子 ：8.000
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Mollugin suppresses proliferation and drives ferroptosis of colorectal cancer cells through inhibition of insulin-like growth factor 2 mRNA binding protein 3/glutathione peroxidase 4 axis(2023/09/05)
作者：Wei Wang, Lijiang Zhou, Xinyu Zhang
期刊：BIOMEDICINE & PHARMACOTHERAPY
DOI：10.1016/j.biopha.2023.115427
影响因子 ：7.500
引用产品：CL-0074
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Norcantharidin Sensitizes Colorectal Cancer Cells to Radiotherapy via Reactive Oxygen Species–DRP1-Mediated Mitochondrial Damage(2024/03/14)
作者：Qiong Xu, Heng Zhang, Haoren Qin
期刊：Antioxidants
影响因子 ：7.000
引用产品：CL-0074
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METTL3-mediated RanGAP1 promotes colorectal cancer progression through the MAPK pathway by recruiting YTHDF1(2024/01/24)
作者：Rui Yang, Cheng Yang, Danjie Su
期刊：CANCER GENE THERAPY
DOI：10.1038/s41417-024-00731-5
影响因子 ：6.400
引用产品：CL-0074
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Identification and validation of a novel prognosis model based on m5C-related long non-coding RNAs in colorectal cancer(2023/09/05)
作者：Ziyang Di, Gaoran Xu, Zheyu Ding
期刊：Cancer Cell International
DOI：10.1186/s12935-023-03025-2
影响因子 ：5.800
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Autophagy Induction by Trichodermic Acid Attenuates Endoplasmic Reticulum Stress-Mediated Apoptosis in Colon Cancer Cells(2021/5/25)
作者：Junyan Qu, Cheng Zeng, Tingting Zou
期刊：International Journal Of Molecular Sciences
影响因子 ：5.600
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SERPINB5 promotes colorectal cancer invasion and migration by promoting EMT and angiogenesis via the TNF-α/NF-κB pathway(2024/03/08)
作者：Bixia Liu, Yang Xie, Jiayu Zhang
期刊：INTERNATIONAL IMMUNOPHARMACOLOGY
DOI：10.1016/j.intimp.2024.111759
影响因子 ：5.600
引用产品：CL-0074
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Raltitrexed as a synergistic hyperthermia chemotherapy drug screened in patient-derived colorectal cancer organoids(2021/8/15)
作者：Lisi Zeng, Quanxing Liao, Haoran Zhao
期刊：Cancer Biology & Medicine
DOI：10.20892/j.issn.2095-3941.2020.0566
影响因子 ：5.500
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miR-145-5p restrained cell growth, invasion, migration and tumorigenesis via modulating RHBDD1 in colorectal cancer via the EGFR-associated signaling pathway(2019/11/03)
作者：Ying Niu, Jinping Zhang, Yalin Tong
期刊：International Journal Of Biochemistry & Cell Biology
DOI：10.1016/j.biocel.2019.105641
影响因子 ：4.000
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Integrated Multi-omics Analyses Identify CDCA5 as a Novel Biomarker Associated with Alternative Splicing, Tumor Microenvironment, and Cell Proliferation in Colon Cancer Via Pan-cancer Analysis(2024/01/01)
作者：Xinyue Bao, Xin Leng, Tianyu Yu
期刊：Journal of Cancer
影响因子 ：3.900
引用产品：CL-0074
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Critical role of guanylate binding protein 5 in tumor immune microenvironment and predictive value of immunotherapy response.(2022/09/30)
作者：Xiang Li, Dan Song, Song Su
期刊：Frontiers in Genetics
影响因子 ：3.700
引用产品：CL-0074
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HSPA5 repressed ferroptosis to promote colorectal cancer development by maintaining GPX4 stability(2022/09/01)
作者：Ronglin Wang, Lei Hua, Peixiang Ma
期刊：NEOPLASMA
DOI：10.4149/neo_2022_220331N363
影响因子 ：3.409
引用产品：CL-0074
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CAMK2N1 has a cancer-suppressive function in colorectal carcinoma via effects on the Wnt/β-catenin pathway(2022/08/15)
作者：Xiaolong Zhang, Lifei Tian, Zeyu Li
期刊：BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
DOI：10.1016/j.bbrc.2022.08.036
影响因子 ：3.100
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RNF114 facilitates the proliferation, stemness, and metastasis of colorectal cancer(2023/07/27)
作者：Shicheng Liu, Lelan Gong, Fengchang Huang
期刊：PATHOLOGY RESEARCH AND PRACTICE
影响因子 ：2.800
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Circ_KIAA1199 inhibits MSI1 degradation by targeting miR-34c-5p to drive the malignant cell behaviors and tumor growth of colorectal cancer(2022/01/01)
作者：Yanbo Zhang, Hailong Yu, Zhen Guo
期刊：Anti-Cancer Drugs
DOI：10.1097/CAD.0000000000001164
影响因子 ：2.300
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