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产品概述
产品信息
别名 | Fibroblast Growth Factor 2; FGF-2; Basic fibroblast Growth Factor; bFGF; Heparin-binding Growth Factor 2; HBGF-2; FGF2; FGFB |
物种 | Human |
表达宿主 | E.coli |
序列信息 | Met1-Ser155 |
检索号 | BAG70264.1 |
分子量 | 17.2 kDa |
表观分子量 | 16 kDa |
产品特性
纯度 | >95% as determined by reducing SDS-PAGE. |
内毒素 | <1.0 EU/µg as determined by LAL test. |
保存 | Lyophilized protein should be stored at -5~-20℃, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8℃ for 2-7 days. Aliquots of reconstituted samples are stable at -5~-20℃ for 3 months. |
运输 | Ambient temperature or ice pack. |
制剂 | Lyophilized from a 0.2 μm filtered solution of 20mM Tris, 5% sucrose, 0.02% Tween 80, pH 8.0. |
复融 | Always centrifuge tubes before opening. Do not mix by vortex or pipetting.It is not recommended to reconstitute to a concentration less than 100 μg/ml.Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
背景介绍
参考文献
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Tumor-suppressive E3 ubiquitin ligase CHIP inhibits the PBK/ERK axis to repress stem cell properties and radioresistance in non-small cell lung cancer (2022-11-27)
作者:Bo Tan:1.Department of Radiotherapy, The Affiliated Cancer Hospital of Zhengzhou University & Henan Cancer Hospital, 127 Dongming Road, Jinshui, Zhengzhou, 450008, Henan, China. zlyytanbo3069@zzu.edu.cn. ; Jingwei Zhang:1.Department of Radiotherapy, The Affiliated Cancer Hospital of Zhengzhou University & Henan Cancer Hospital, 127 Dongming Road, Jinshui, Zhengzhou, 450008, Henan, China. ; Wen Wang:1.Department of Radiotherapy, The Affiliated Cancer Hospital of Zhengzhou University & Henan Cancer Hospital, 127 Dongming Road, Jinshui, Zhengzhou, 450008, Henan, China. ; Haibo Ma:1.Department of Thoracic Surgery, The Affiliated Cancer Hospital of Zhengzhou University & Henan Cancer Hospital, Zhengzhou, 450008, China. ; Yuanyuan Yang:1.Department of Radiotherapy, The Affiliated Cancer Hospital of Zhengzhou University & Henan Cancer Hospital, 127 Dongming Road, Jinshui, Zhengzhou, 450008, Henan, China. ;
期刊:Tumor-suppressive E3 ubiquitin ligase CHIP inhibits the PBK/ERK axis to repress stem cell properties and radioresistance in non-small cell lung cancer
DOI:10.1007/s10495-022-01789-y
影响因子 :5.6
引用产品: PC-9 细胞 , A549 [A-549] 细胞 , NCI-H1299 细胞 , NCI-H1975 细胞 , BEAS-2B 细胞 , RPMI-1640 培养基 , BEAS-2B细胞专用培养基 , EGF/Urogastrone/URG, Human, Recombinant , FGF-2/ bFGF/ FGF-b (Q65I, C96S, N111G), Human, Recombinant
FAQs
Q:收到细胞因子产品用什么溶剂复融?
A:1、细胞因子产品大部分为冻干形式发货,少部分液体形式发货; 2、液体形式因子产品不需要复溶,具体浓度详见产品标签,按照实验需要用含载体蛋白(如0.1% BSA,10%FBS,5%HSA 或 5% 海藻糖)的溶液进一步稀释后分装冷冻保存即可; 3、冻干粉状态的因子产品需指定溶剂复溶,请参考产品说明书【复融】方法,按照要求使用对应溶剂溶解,部分需要无菌水以外的特殊溶剂溶解的产品则随货会赠送相应的复融试剂;冻干粉溶解后如您需要进一步稀释分装,需使用含载体蛋白(如0.1% BSA,10%FBS,5%HSA 或 5% 海藻糖)的溶液进一步稀释后分装冷冻保存。
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