本司产品仅用于科研,不用于临床诊断和治疗

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  • 0.25%胰蛋白酶溶液(含EDTA,溶于PBS)
  • 0.25%胰蛋白酶溶液(含EDTA,溶于PBS)
  • 0.25%胰蛋白酶溶液(含EDTA,溶于PBS)

0.25%胰蛋白酶溶液(含EDTA,溶于PBS)

货号:PB180225

规格:125mL/500mL

价格: ¥68/¥228

数量: - +
  • 0.25%胰蛋白酶溶液(含EDTA,溶于PBS)
  • 0.25%胰蛋白酶溶液(含EDTA,溶于PBS)
  • 0.25%胰蛋白酶溶液(含EDTA,溶于PBS)
产品概述

胰蛋白酶(Trypsin)是一种丝氨酸水解酶,它能把多肽链中赖氨酸和精氨酸残基中的羧基侧切段,水解细胞间的蛋白质,破坏细胞间的连接,从而使组织或贴壁细胞离散成单个细胞。胰酶分散细胞的活性与组织或细胞的特性、胰酶浓度、温度和作用时间有关,在PH 8.037℃时,胰酶的作用能力最强,因此使用胰酶时,应把握好浓度、温度和时间,以免消化过度造成细胞损伤。

一般常用胰酶的工作浓度为0.25%,而半贴壁细胞或对胰酶敏感的细胞常采用低浓度(0.05%)的胰酶进行细胞消化。由于EDTA能够螯合Ca2+Mg2+,从而破坏细胞连接促进细胞的解离,因此在胰酶溶液中常常会加入一定量的EDTA混合使用,以增强解离效果。

产品信息

形态

液体

规格

125mL/500mL

溶剂

10mM PBS

胰蛋白酶浓度

2.5g/L

EDTA2Na

0.2g/L

酚红指示剂

储存条件

-5~-20

运输条件

低温

有效期

12个月

注意事项

1、由于不同的组织或者细胞对胰酶的作用反应不一样,因此操作人员应根据实际情况,确定最佳消化时间;消化细胞时间不宜过长,否则会影响细胞贴壁和生长状况;

2、使用本产品时应注意无菌操作,避免污染;

3、不宜长时间放置于室温环境或4℃长期保存;

42-8℃中解冻,摇匀后使用,切忌反复冻融,用量较少时建议分装冻存。

5、本产品仅用于科研或进一步研究使用,不用于诊断和治疗。

产品手册
参考文献
文献列表
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