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产品概述
名称 | MDA-MB-453 (人乳腺癌细胞) (STR鉴定正确) |
别称 | MDA-MB 453; MDA MB 453; MDA-MB453; MDAMB453; MDA-453; MDA453; MD Anderson-Metastatic Breast-453 |
种属 | 人类 |
生长特性 | 半贴半悬 |
细胞形态 | 上皮细胞样 |
冻存条件 | 冻存液:55% 基础培养基+40%FBS+5%DMSO 温度:液氮 |
培养方案A(默认) |
生长培养基:
培养条件:
气相:空气,100%, 温度:37℃
|
培养方案B(可选) |
培养条件:
气相:空气,95%;CO2,5%, 温度:37℃
|
推荐传代比例 | 1:2-1:4 |
推荐换液频率 | 2-3次/周 |
注意事项 | 1.该细胞推荐使用Leibovitz's L-15培养基进行培养,Leibovitz's L-15不可以通入二氧化碳,会产生细胞毒性;2.如您没有无二氧化碳的培养箱,可使用DMEM替代Leibovitz's L-15,使用DMEM培养基时即可正常通入5%二氧化碳;3.配套专用培养基默认Leibovitz's L-15配置,如需DMEM配方,请联系销售下单备注更改。 |
背景描述 | MDA-MB-453细胞是由R·Cailleau等在1976年从一位48岁女性肿瘤转移患者胸水中建立的细胞株,其它转移灶包括淋巴结、脑和胸水及心包腔积水;MDA-MB-453细胞过表达FGF受体。 |
年龄(性别) | 女性,48岁 |
组织来源 | 乳腺;源自转移部位:胸腔积液 |
细胞类型 | 肿瘤细胞 |
肿瘤类型 | 乳腺癌细胞 |
生物安全等级 | BSL-1 |
倍增时间 | ~26-38 hours |
致瘤性 | No, in immunosuppressed mice.Yes, in semisolid medium. |
受体表达情况 | fibroblast growth factor (FGF), expressed |
保藏机构 | ATCC; HTB-131 |
STR鉴定
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STR位点信息
Amelogenin X CSF1PO 10,12 D2S1338 23,24 D3S1358 15 D5S818 11 D7S820 10 D8S1179 10,12 D13S317 12 D16S539 9 D18S51 15,20 D19S433 13,14 D21S11 29,31 FGA 18,23 PentaD 9,10 PentaE 11 TH01 6 TPOX 10 vWA 17,18 D6S1043 11,18 D12S391 18,21 D2S441 10 -
STR鉴定图
参考文献
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CircFBXL5 promotes the 5-FU resistance of breast cancer via modulating miR-216b/HMGA2 axis(2021/07/19)
作者:Mingzhi Zhu, Yanyan Wang, Fang Wang
期刊:Cancer Cell International
DOI:10.1186/s12935-021-02088-3
影响因子 :5.800
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A Novel Optical Fiber Terahertz Biosensor Based on Anti-Resonance for The Rapid and Nondestructive Detection of Tumor Cells(2023/10/23)
作者:Zhe He, Yueping Luo, Guorong Huang
期刊:Biosensors-Basel
影响因子 :5.400
引用产品:CL-0152
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Circular RNA circ-ERBB2 Elevates the Warburg Effect and Facilitates Triple-Negative Breast Cancer Growth by the MicroRNA 136-5p/Pyruvate Dehydrogenase Kinase 4 Axis | Molecular and Cellular Biology(2021/09/24)
作者:Yihong Huang, Shuo Zheng, Ying Lin, Liming Kea
期刊:Molecular And Cellular Biology
影响因子 :5.300
引用产品:164210 CL-0041 CL-0150 CL-0152 CL-0290 CL-0525 PB180120 PM150110 PM151010
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Circular RNA circ-ERBB2 Elevates the Warburg Effect and Facilitates Triple-Negative Breast Cancer Growth by the MicroRNA 136-5p/Pyruvate Dehydrogenase Kinase 4 Axis(2023/03/03)
作者:Yihong Huang, Shuo Zheng, Ying Lin
期刊:MOLECULAR AND CELLULAR BIOLOGY
影响因子 :5.300
引用产品:CL-0152
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4-Amino-2-trifluoromethyl-phenyl retinate inhibits proliferation, invasion, and migration of breast cancer cells by independently regulating CRABP2 and FABP5(2022/10/05)
作者:Jing Ju, Nan Wang, Jiali Wang
期刊:Drug Design Development and Therapy
影响因子 :4.319
引用产品:CL-0152
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Enantiomeric α-pyrone derivatives with immunosuppressive activity from Talaromyces adpressus(2023/11/27)
作者:Meijia Zheng, Chenxi Zhou, Hong Liao
期刊:PHYTOCHEMISTRY
DOI:10.1016/j.phytochem.2023.113931
影响因子 :3.800
引用产品:CL-0152
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Isopimaric acid, an ion channel regulator, regulates calcium and oxidative phosphorylation pathways to inhibit breast cancer proliferation and metastasis(2023/02/06)
作者:Jiacheng Li, Xiaozhen Liu, Lin Chen
期刊:TOXICOLOGY AND APPLIED PHARMACOLOGY
DOI:10.1016/j.taap.2023.116415
影响因子 :3.800
引用产品:CL-0007 CL-0093 CL-0149 CL-0150 CL-0152 CL-0290 CL-0525
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circKLHL24 Blocks Breast Cancer Development by Regulating the miR-1204/ALX4 Network(2022/10/12)
作者:Guangming Yi, Dan Wang, Jianjun Han
期刊:CANCER BIOTHERAPY AND RADIOPHARMACEUTICALS
影响因子 :3.632
引用产品:CL-0152
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Sericin inhibits MDA‑MB‑468 cell proliferation via the PI3K/Akt pathway in triple‑negative breast cancer(2020/12/13)
作者:Lin Niu, Songhe Yang, Xueying Zhao
期刊:Molecular Medicine Reports
影响因子 :3.400
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