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产品概述
Leibovitz's L-15培养基的缓冲系统由磷酸盐和游离碱基氨基酸组成,代替了传统的碳酸氢钠缓冲系统,同时,用半乳糖和丙酮酸钠代替葡萄糖可以防止酸性代谢副产物的形成,有助于维持培养液PH的稳定,适合用于非CO2平衡环境的细胞培养。Leibovitz's L-15培养基适用于猴肾细胞和HEP-2的培养、原代(如胚胎和成人组织)细胞分离。
本产品含有多类细胞培养所需的氨基酸、维生素、无机盐等多种成分,但不含蛋白质、脂类或任何生长因子,故此产品需搭配血清或无血清添加物使用。
成分说明
形态 | 液体 |
浓度 | 1× |
规格 | 500mL |
pH | 7.2-7.4 |
缓冲系统 | 磷酸盐、氨基酸缓冲系统 |
L-谷氨酰胺 | 300 mg/L |
NaHCO3 | 无 |
D-葡萄糖 | 无 |
D-半乳糖 | 900 mg/L |
丙酮酸钠 | 550 mg/L |
HEPES缓冲剂 | 无 |
酚红指示剂 | 10 mg/L |
储存条件 | 2-8℃,避光 |
运输条件 | 常温 |
有效期 | 18个月 |
注意事项
1、本产品经0.1 μm过滤除菌,使用时应注意无菌操作,避免污染;
2、本品不适用于CO2环境,CO2会导致培养液PH降低(培养液变黄),严重影响细胞生长;
3、为保持本产品的最佳使用效果,请勿进行冻融处理;
4、本产品仅用于科研或进一步研究使用,不用于诊断和治疗。
参考文献
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In vivo imaging of mitochondrial DNA mutations using an integrated nano Cas12a sensor(2023/11/24)
作者:Yanan Li, Yonghua Wu, Ru Xu
期刊:Nature Communications
DOI:10.1038/s41467-023-43552-0
影响因子 :16.600
引用产品:PM151010
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Cytoplasmic YAP1-mediated ESCRT-III assembly promotes autophagic cell death and is ubiquitinated by NEDD4L in breast cancer(2023/04/02)
作者:Yan Guo, Yuqing Cui, Yangyang Li
期刊:Cancer Communications
影响因子 :16.200
-
Circ-MBOAT2 knockdown represses tumor progression and glutamine catabolism by miR-433-3p/GOT1 axis in pancreatic cancer(2021/04/08)
作者:Zhou Xiaoxiao, Liu Kun, Cui Jing
期刊:Journal of Experimental & Clinical Cancer Research
DOI:10.1186/s13046-021-01894-x
影响因子 :11.300
-
The perturbation of parabens on the neuroendocrine system in zebrafish larvae(2023/04/21)
作者:Jiefeng Liang, Xiaoxi Yang, Tongtong Xiang
期刊:SCIENCE OF THE TOTAL ENVIRONMENT
DOI:10.1016/j.scitotenv.2023.163593
影响因子 :9.800
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WD repeat protein 54-mediator of ErbB2-driven cell motility 1 axis promotes bladder cancer tumorigenesis and metastasis and impairs chemosensitivity(2023/01/07)
作者:Xiaosong Wei, Beibei Wang, Zixin Wu
期刊:CANCER LETTERS
DOI:10.1016/j.canlet.2023.216058
影响因子 :9.700
引用产品:CL-0002 CL-0125 CL-0222 CL-0227 CL-0463 CL-0628 PM150410 PM150710 PM150910 PM151010
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Exosomal miR-625-3p secreted by cancer-associated fibroblasts in colorectal cancer promotes EMT and chemotherapeutic resistance by blocking the CELF2/WWOX pathway(2022/11/03)
作者:Ying Zhang, Changjun Yin, Chao Wei, Shuai Xia, Zhu Qiao, Xue-Wen Zhang, Bin Yu, Jun Zhou, Rong Wang
期刊:PHARMACOLOGICAL RESEARCH
DOI:10.1016/j.phrs.2022.106534
影响因子 :9.300
引用产品:PM151010
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CL4-modified exosomes deliver lncRNA DARS-AS1 siRNA to suppress triple-negative breast cancer progression and attenuate doxorubicin resistance by inhibiting autophagy(2023/08/05)
作者:Xinli Liu, Ge Zhang, Tongyao Yu
期刊:INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
DOI:10.1016/j.ijbiomac.2023.126147
影响因子 :8.200
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PSMD12 interacts with CDKN3 and facilitates pancreatic cancer progression(2023/04/10)
作者:Jia Ma, Wenyang Zhou, Yifeng Yuan
期刊:Cancer Gene Therapy
DOI:10.1038/s41417-023-00609-y
影响因子 :6.400
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An antihypertensive drug-AT1 inhibitor attenuated BRCA development promoted by chronic psychological stress via Ang II/PARP1/FN1 pathway(2024/01/20)
作者:Yuqing Cui, Ming Zhuang, Zheping Huang
期刊:BIOCHIMICA ET BIOPHYSICA ACTA-MOLECULAR BASIS OF DISEASE
DOI:10.1016/j.bbadis.2024.167031
影响因子 :6.200
引用产品:PM151010
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Angelica sinensis polysaccharide suppresses the Wnt/β-catenin-mediated malignant biological behaviors of breast cancer cells via the miR-3187-3p/PCDH10 axis(2024/05/16)
作者:Yan Cai, Yang Wang, Wenjun Su
期刊:BIOCHEMICAL PHARMACOLOGY
影响因子 :5.800
引用产品:PM151010
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Bioactive Components and Potential Mechanisms of Biqi Capsule in the Treatment of Osteoarthritis: Based on Chondroprotective and Anti-inflammatory Activity(2024/04/01)
作者:Ziyue Jia, Xintong Yang, Huiyou Chen
期刊:Frontiers in Pharmacology
DOI:10.3389/fphar.2024.1347970
影响因子 :5.600
引用产品:PM151010
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Circular RNA circ-ERBB2 Elevates the Warburg Effect and Facilitates Triple-Negative Breast Cancer Growth by the MicroRNA 136-5p/Pyruvate Dehydrogenase Kinase 4 Axis | Molecular and Cellular Biology(2021/09/24)
作者:Yihong Huang, Shuo Zheng, Ying Lin, Liming Kea
期刊:Molecular And Cellular Biology
影响因子 :5.300
引用产品:164210 CL-0041 CL-0150 CL-0152 CL-0290 CL-0525 PB180120 PM150110 PM151010
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Circular RNA circ-ERBB2 Elevates the Warburg Effect and Facilitates Triple-Negative Breast Cancer Growth by the MicroRNA 136-5p/Pyruvate Dehydrogenase Kinase 4 Axis(2023/03/03)
作者:Yihong Huang, Shuo Zheng, Ying Lin
期刊:MOLECULAR AND CELLULAR BIOLOGY
影响因子 :5.300
引用产品:PM151010
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GID2 interacts with CDKN3 and regulates pancreatic cancer growth and apoptosis(2023/02/23)
作者:Xin Deng, Jia Ma, Wenyang Zhou
期刊:LABORATORY INVESTIGATION
DOI:10.1016/j.labinv.2023.100122
影响因子 :5.000
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Fat mass and obesity-associated protein (FTO) mediates signal transducer and activator of transcription 3 (STAT3)-drived resistance of breast cancer to doxorubicin(2021/06/02)
作者:Yan Wang, Zhiqiang Cheng, Jing Xu
期刊:Bioengineered
DOI:10.1080/21655979.2021.1924544
影响因子 :4.900
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Timosaponin B-II alleviates osteoarthritis-related inflammation and extracellular matrix degradation through inhibition of mitogen-activated protein kinases and nuclear factor-κB pathways in vitro(2022/01/30)
作者:Xinwei Liu, Dulei Xiang, Wenming Jin
期刊:Bioengineered
DOI:10.1080/21655979.2021.2024685
影响因子 :4.900
引用产品:PM151010
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Deubiquitinating enzyme PSMD7 promotes bladder cancer development: Involvement of RAB1A stabilization(2023/11/30)
作者:Jun Wang, Tao Wang, Yuankang Feng
期刊:CELLULAR SIGNALLING
DOI:10.1016/j.cellsig.2023.110996
影响因子 :4.800
引用产品:PM151010
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PDIA6 promotes pancreatic cancer progression and immune escape through CSN5-mediated deubiquitination of β-catenin and PD-L1(2021/07/26)
作者:Yihui Ma, Peiyi Xia, Zhengyang Wang
期刊:Neoplasia
DOI:doi.org/10.1016/j.neo.2021.07.004
影响因子 :4.800
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ARHGAP25 suppresses the development of breast cancer by an ARHGAP25/Wnt/ASCL2 feedback loop(2023/06/16)
作者:Sijia Han, Xueying Jin, Tianyu Hu
期刊:CARCINOGENESIS
影响因子 :4.700
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Induction of oocyte maturation and changes in the biochemical composition, physiology and molecular biology of oocytes during maturation and hydration in the orange-spotted grouper (Epinephelus coioides)(2020/2/12)
作者:Lin Tang, Xinxun Xiao, Herong Shi
期刊:Aquaculture
DOI:10.1016/j.aquaculture.2020.735115
影响因子 :4.500
引用产品:PM151010
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Preliminary study on the main mode of the death of red blood cells in grass carp (Ctenopharyngodon idella)(2022/11/24)
作者:Hong Chen, Minshan Yao, Yin Tang, Lixiang Wei, Junyan Lin, Fei Shi, Fanbin Zhan, Yanan Li, Jun Li, Li Lin, Zhendong Qin
期刊:Aquaculture
DOI:10.1016/j.aquaculture.2022.739071
影响因子 :4.500
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Circ_0056618 enhances PRRG4 expression by competitively binding to miR-411-5p to promote the malignant progression of colorectal cancer(2022/08/02)
作者:Bo Zhang, Wenbin Cao, Yang Liu
期刊:MOLECULAR AND CELLULAR BIOCHEMISTRY
DOI:10.1007/s11010-022-04525-x
影响因子 :4.300
引用产品:164210 164210-50 CL-0096 CL-0144 CL-0223 CL-0225 PM150710 PM151010
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Pseudolaric acid B induces apoptosis associated with the mitochondrial and PI3K/AKT/mTOR pathways in triple‑negative breast cancer(2023/09/14)
作者:Ke Yang, Junqi Wang, Kai Li
期刊:ONCOLOGY REPORTS
影响因子 :4.200
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LAPTM5 regulated by FOXP3 promotes the malignant phenotypes of breast cancer through activating the Wnt/β‑catenin pathway(2023/02/10)
作者:Sijia Han, Xueying Jin, Tianyu Hu, Feng Chi
期刊:ONCOLOGY REPORTS
影响因子 :4.200
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MiR-519d and miR-328-3p Combinatorially Suppress Breast Cancer Progression(2023/04/07)
作者:Haiming Ma, Tao Liu, Yanhua Xu
期刊:OncoTargets and Therapy
影响因子 :4.000
引用产品:PM151010
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The therapeutic effect and targets of herba Sarcandrae on breast cancer and the construction of a prognostic signature consisting of inflammation-related genes(2024/05/10)
作者:Jie Yuan, Minxia Lin, Shaohua Yang
期刊:Heliyon
DOI:10.1016/j.heliyon.2024.e31137
影响因子 :4.000
引用产品:PM151010
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hsa_circ_0020378 regulating miR-339-3p/COL1A1 promotes osteosarcoma progression(2023/11/18)
作者:Liang Liu, Wenze Huang
期刊:CANCER BIOLOGY & THERAPY
DOI:10.1080/15384047.2023.2274120
影响因子 :3.600
引用产品:PM151010
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STAM binding protein regulated by hsa_circ_0007334 exerts oncogenic potential in pancreatic cancer(2022/08/30)
作者:Shan Yu, Changyong E, Jinghui Yang
期刊:PANCREATOLOGY
影响因子 :3.600
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Isoliquiritin modulates ferroptosis via NF-κB signalling inhibition and alleviates doxorubicin resistance in breast cancer(2023/01/05)
作者:Jiguo Wang, Yang Li, Jing Zhang
期刊:IMMUNOPHARMACOLOGY AND IMMUNOTOXICOLOGY
DOI:10.1080/08923973.2023.2165943
影响因子 :3.300
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Karyopherin Subunit Alpha 1 Enhances the Malignant Behaviors of Colon Cancer Cells via Promoting Nuclear Factor-κB p65 Nuclear Translocation(2023/04/10)
作者:Lianrong Zhao, Di Wu, Qiao Qu
期刊:DIGESTIVE DISEASES AND SCIENCES
DOI:10.1007/s10620-023-07936-y
影响因子 :3.100
引用产品:PM151010
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The New Role of HNF1A-NAS1/miR-214/INHBA Signaling Axis in Colorectal Cancer(2023/11/24)
作者:Xuan Zhang, Tao Wu, Rujia Qin
期刊:Frontiers in Bioscience-Landmark
影响因子 :3.100
引用产品:PM151010
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Stachydrine represses the proliferation and enhances cell cycle arrest and apoptosis of breast cancer cells via PLA2G2A/DCN axis(2024/01/01)
作者:Zhen Zhai, Tianlong Mu, Lina Zhao
期刊:Chemical Biology & Drug Design
影响因子 :3.000
引用产品:PM151010
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Transcriptomic Analysis Revealed the Regulatory Mechanisms of Oocyte Maturation and Hydration in Orange-Spotted Grouper (Epinephelus coioides)(2019/05/25)
作者:Lin Tang, Jiaxing Chen, Zhifeng Ye
期刊:Marine Biotechnology
DOI:10.1007/s10126-019-09902-0
影响因子 :3.000
引用产品:PM151010
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Apolipoprotein A-1 downregulation promotes basal-like breast cancer cell proliferation and migration associated with DNA methylation(2024/05/01)
作者:Cong Wang, Shiliang Chen, Ranran Zhang
期刊:Oncology Letters
影响因子 :2.900
引用产品:PM151010
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Tumor-promoting properties of enolase-phosphatase 1 in breast cancer via activating the NF-κB signaling pathway(2022/11/15)
作者:Bu, Yuhui, Hao, Jun, He, Jianchao, Li, Xiaolong, Liu, Yinfeng, Ma, Li
期刊:MOLECULAR BIOLOGY REPORTS
DOI:10.1007/s11033-022-08066-w
影响因子 :2.800
引用产品:PM151010
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microRNA-4429-5p suppresses the malignant development of colon cancer by targeting matrix metalloproteinase 16(2021/08/26)
作者:Wei Li, Zhe Song, Nan Jia
期刊:In Vitro Cellular & Developmental Biology-Animal
DOI:10.1007/s11626-021-00603-4
影响因子 :2.723
引用产品:CL-0050 CL-0096 CL-0144 CL-0223 CL-0225 PM150710 PM150910 PM151010
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Circ_0007422 Knockdown Inhibits Tumor Property and Immune Escape of Colorectal Cancer by Decreasing PDL1 Expression in a miR-1256-Dependent Manner(2024/01/22)
作者:Dian Yin, Li Yang, Xiu Feng
期刊:MOLECULAR BIOTECHNOLOGY
DOI:10.1007/s12033-023-01040-2
影响因子 :2.600
引用产品:PM151010
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Resveratrol Inhibits Colorectal Cancer Cell Tumor Property by Activating the miR-769-5p/MSI1 Pathway(2024/05/21)
作者:Hongchang Liu, Liangliang Zhang, Liangliang Hao
期刊:MOLECULAR BIOTECHNOLOGY
DOI:10.1007/s12033-024-01167-w
影响因子 :2.600
引用产品:PM151010
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Zinc finger protein 468 up-regulation of TFAM contributes to the malignant growth and cisplatin resistance of breast cancer cells(2024/03/01)
作者:Zhaoyang Jia, Feng Wang, Gongzhuo Li
期刊:Cell Division
DOI:10.1186/s13008-024-00113-1
影响因子 :2.300
引用产品:PM151010
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Hsa_circ_0069094 positively regulates the expression of oncogenic ZNF217 by competitively targeting miR-758–3p to promote the development of breast cancer(2022/11/08)
作者:Huaqing Ou, Jialing Li, Qinhua Lv, Decai Feng
期刊:Reproductive Biology
DOI:10.1016/j.repbio.2022.100708
影响因子 :2.100
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Inhibition of glycolysis represses the growth and alleviates the endoplasmic reticulum stress of breast cancer cells by regulating TMTC3(2023/04/10)
作者:Xue Hu, Baoliang Guo, Tong Sun
期刊:Open Medicine
影响因子 :2.100
引用产品:164210 164215 CL-0149 CL-0150 CL-0211 CL-0383 CL-0525 PB180120 PB180424 PM150140 PM150312 PM150710 PM151010
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GLIS Family Zinc Finger 3 Promotes Triple-Negative Breast Cancer Progression by Inducing Cell Proliferation, Migration and Invasion, and Activating the NF-κB Signaling Pathway(2023/02/01)
作者:Chenhao Li, Cuizhi Geng
期刊:BIOLOGICAL & PHARMACEUTICAL BULLETIN
影响因子 :2.000
引用产品:PM151010
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