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产品概述
| 名称 | B16 (小鼠黑色素瘤细胞) (STR鉴定正确) |
| 别称 | B-16; B16 melanoma; B16 subline B78; B78 |
| 种属 | C57BL/6小鼠 |
| 生长特性 | 贴壁细胞 |
| 细胞形态 | 成纤维细胞样 |
| 冻存条件 | 冻存液：55% 基础培养基+40%FBS+5%DMSO 温度：液氮 |
| 培养方案A（默认） |
培养条件:
气相：空气，95%；CO2，5%, 温度：37℃
|
| 推荐传代比例 | 1:3-1:4 |
| 推荐换液频率 | 2-3次/周 |
| 注意事项 | B16系列细胞，使用DMEM培养时可能出现黑色素快速积累，细胞不增殖或死亡的情况，该细胞正常扩增冻存请使用RPMI-1640完培，若您需要进行分泌黑色素等后续相关实验，可以在扩增冻存细胞后更换为DMEM培养来满足实验设计。 |
| 背景描述 | B16细胞是一株小鼠黑素瘤细胞。 |
| 年龄（性别） | 不详 |
| 组织来源 | 黑素瘤 |
| 细胞类型 | 肿瘤细胞 |
| 肿瘤类型 | 黑色素瘤细胞 |
| 生物安全等级 | BSL-1 |
| 保藏机构 | JCRB; JCRB0202 KCB; KCB 93030YJ RCB; RCB1283 TKG; TKG 0144 |
STR鉴定
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STR位点信息
18-3 15,16 6-7 15,16 5-5 16,20 X-1 28 1-2 19,20 7-1 26.2 8-1 16,17 1-1 17,18 19-2 13 15-3 22.3,23.3 12-1 17,18 6-4 18,19 4-2 20.3,21.3 3-2 14,15 2-1 16 13-1 17.1,18.1
11-2 16,17 17-2 15,16,17 -
STR鉴定图
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参考文献
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In Situ Self-Assembly Nanomicelle Microneedles for Enhanced Photoimmunotherapy via Autophagy Regulation Strategy(2021/02/23)
作者：Minglong Chen, Dan Yang, Ying Sun
期刊：ACS Nano
影响因子 ：17.100
引用产品：CL-0029
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Impact of particle size and pH on protein corona formation of solid lipid nanoparticles: A proof-of-concept study(2020/10/29)
作者：Wenhao Wang, Zhengwei Huang, Yanbei Li
期刊：Acta Pharmaceutica Sinica B
DOI：10.1016/j.apsb.2020.10.023
影响因子 ：14.500
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CD44 targeting nanodrug based on chondroitin sulfate for melanoma therapy by inducing mitochondrial apoptosis pathways(2023/08/02)
作者：Yan Li, Huiwen Hou, Zengmei Liu
期刊：CARBOHYDRATE POLYMERS
DOI：10.1016/j.carbpol.2023.121255
影响因子 ：11.200
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Microneedle-assisted vaccination combined with autophagy regulation for antitumor immunotherapy(2023/04/24)
作者：Dan Yang, Minglong Chen, Ying Sun
期刊：JOURNAL OF CONTROLLED RELEASE
DOI：10.1016/j.jconrel.2023.04.031
影响因子 ：10.800
引用产品：CL-0029
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ATP-adenosine axis regulation combined with microneedle assisted photoimmunotherapy to boost the immunotherapy efficiency(2024/01/23)
作者：Chaonan Shi, Minglong Chen, Xiaodie Li
期刊：JOURNAL OF CONTROLLED RELEASE
DOI：10.1016/j.jconrel.2024.01.035
影响因子 ：10.800
引用产品：CL-0029
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A novel multifunctional microneedle patch for synergistic photothermal- gas therapy against maxillofacial malignant melanoma and associated skin defects(2024/04/23)
作者：Shaojie Dong, Yuwei Zhang, Yifei Zhang
期刊：JOURNAL OF NANOBIOTECHNOLOGY
DOI：10.1186/s12951-024-02409-4
影响因子 ：10.200
引用产品：CL-0029
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Extracellular matrix modulating enzyme functionalized biomimetic Au nanoplatform-mediated enhanced tumor penetration and synergistic antitumor therapy for pancreatic cancer(2022/12/10)
作者：Xiaoyan Yang, Jinguo Zhang, Qiaomei Zhou
期刊：JOURNAL OF NANOBIOTECHNOLOGY
DOI：10.1186/s12951-022-01738-6
影响因子 ：10.200
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Delivery of mRNA Vaccine with 1, 2-Diesters-Derived Lipids Elicits Fast Liver Clearance for Safe and Effective Cancer Immunotherapy(2023/11/21)
作者：Yuandong Xu, Yuexiao Hu, Heng Xia
期刊：Advanced Healthcare Materials
影响因子 ：10.000
引用产品：CL-0029
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Synthesis of iron-boride/carbon-nitride composites and their applications in chemodynamic therapy(2023/12/14)
作者：Xiaoran Xu, Haixu Zhao, JiaJia Wang
期刊：JOURNAL OF COLLOID AND INTERFACE SCIENCE
DOI：10.1016/j.jcis.2023.12.078
影响因子 ：9.900
引用产品：CL-0029
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Janus-Inspired Core–Shell Structure Hydrogel Programmatically Releases Melatonin for Reconstruction of Postoperative Bone Tumor(2023/01/05)
作者：Wei Huang, Xiaoyue Wu, Yifan Zhao
期刊：ACS Applied Materials & Interfaces
影响因子 ：9.500
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High-contrast tumor imaging via a de novo designed aminopeptidase N targeted fluorogenic probe(2023/12/04)
作者：Xing Gao, Chengyu Fan, Huiling Wang
期刊：SENSORS AND ACTUATORS B-CHEMICAL
影响因子 ：8.400
引用产品：CL-0029
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In situ administration of temperature-sensitive hydrogel composite loading paclitaxel microspheres and cisplatin for the treatment of melanoma(2023/02/10)
作者：Yanlin Liu, Wenqiong Ma, Ping Zhou, Qian Wen, Qinglian Wen, Yun Lu, Ling Zhao, Huan Shi, Jie Dai, Jianmei Li, Shaozhi Fu
期刊：BIOMEDICINE & PHARMACOTHERAPY
DOI：10.1016/j.biopha.2023.114380
影响因子 ：7.500
引用产品：CL-0029
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Dissolving microneedles integrated with pH-responsive micelles containing AIEgen with ultra-photostability for enhancing melanoma photothermal therapy(2020/09/10)
作者：Sihui Wei, Guilan Quan, Chao Lu
期刊：Biomaterials Science
影响因子 ：6.600
引用产品：CL-0029
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Promotion of Melanoma Cell Proliferation by Cyclic Straining through Regulatory Morphogenesis(2022/10/06)
作者：Siyuan Huang, Zhu Chen, Xiaoqiang Hou
期刊：INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
影响因子 ：5.600
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A Digital Controlled Pulse Generator for a Possible Tumor Therapy Combining Irreversible Electroporation With Nanosecond Pulse Stimulation(2020/04/17)
作者：Xin Rao, Xiaodong Chen, Jun Zhou
期刊：IEEE Transactions on Biomedical Circuits and Systems
DOI：10.1109/TBCAS.2020.2987376
影响因子 ：5.100
引用产品：CL-0029
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A colorimetric and near -infrared ratiometric fluorescent probe for the determination of endogenous tyrosinase activity based on cyanine aggregation(2019/09/21)
作者：Peng Zhang, Shasha Li, Caixia Fu
期刊：Analyst
影响因子 ：4.200
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Peptide OA-VI12 restrains melanogenesis in B16 cells and C57B/6 mouse ear skin via the miR-122-5p/Mitf/Tyr axis(2023/10/04)
作者：Junsong Wang, Yilin Li, Chengan Feng
期刊：AMINO ACIDS
DOI：10.1007/s00726-023-03341-x
影响因子 ：3.500
引用产品：CL-0029
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Newly identified peptide Nigrocin-OA27 inhibits UVB induced melanin production via the MITF/TYR pathway(2024/04/11)
作者：Jiayi Li, Saige Yin, Ziqi Wei
期刊：PEPTIDES
DOI：10.1016/j.peptides.2024.171215
影响因子 ：3.000
引用产品：CL-0029
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CREB1 regulates KPNA2 by inhibiting mir-495-3p transcription to control melanoma progression(2022/12/15)
作者：Xuerui Geng, Xiujuan Qiu, Jun Gao
期刊：BMC Molecular and Cell Biology
DOI：10.1186/s12860-022-00446-1
影响因子 ：2.800
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