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产品概述
| 名称 | TM4 (正常小鼠睾丸Sertoli细胞) (STR鉴定正确) |
| 别称 | TM-4 |
| 种属 | 小鼠 |
| 生长特性 | 贴壁细胞 |
| 细胞形态 | 上皮细胞样 |
| 冻存条件 | 冻存液：55% 基础培养基+40%FBS+5%DMSO 温度：液氮 |
| 培养方案A（默认） |
培养条件:
气相：空气，95%；CO2，5%, 温度：37℃
|
| 推荐传代比例 | 1:3-1:4 |
| 推荐换液频率 | 2-3次/周 |
| 注意事项 | 该细胞贴壁松散，操作时请尽量轻柔；换液时需预热培养基；收货如有大块脱落的细胞团，为正常现象，请按照收货注意事项处理。 |
| 背景描述 | TM4细胞在FSH的作用下cAMP产量增加，但对LH无反应；TM4细胞与原代Sertoli细胞相比，对FSH的反应性降低。据报道，TM4细胞组成性纤溶酶原激活物的产量很低，但可被FSH刺激产生，受维甲酸刺激可有大幅增高。TM4细胞可产生视黄醇结合蛋白、组织纤溶酶原激活物和转铁蛋白；TM4细胞表达FSH受体、雄激素受体和孕激素受体；鼠痘病毒阴性。 |
| 年龄（性别） | 雄性；11-13日龄 |
| 组织来源 | 睾丸 |
| 细胞类型 | 自发永生化细胞 |
| 生物安全等级 | BSL-1 |
| 致瘤性 | No, The cells were not tumorigenic in immunosuppressed mice, but did form colonies in semisolid medium. |
| 基因表达情况 | retinol binding protein, tissue plasminogen activator, transferrin |
| 保藏机构 | ATCC; CRL-1715 BCRC; 60254 ECACC; 88111401 |
STR鉴定
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STR位点信息
18-3 18 6-7 12,15 5-5 15 X-1 24,25 1-2 15,17 7-1 24.2,26.2 8-1 16 1-1 15,16 19-2 9 15-3 20.3,21.3,22.3 12-1 20 6-4 18,19 4-2 19.3 3-2 11,14 2-1 9,16 13-1 15.1
11-2 15 17-2 15 -
STR鉴定图
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参考文献
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Environmental cadmium impairs blood-testis barrier via activating HRI-responsive mitochondrial stress in mice(2021/12/09)
作者：Guoxiang Zhou, Weibo Liu, Limin Dai
期刊：Science Of The Total Environment
DOI：10.1016/j.scitotenv.2021.152247
影响因子 ：10.753
引用产品：CL-0456
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Omega-3 polyunsaturated fatty acids and its metabolite 12-HEPE rescue busulfan disrupted spermatogenesis via target to GPR120(2023/09/24)
作者：Jing Jun, Ouyang Lei, Zhang Hong
期刊：CELL PROLIFERATION
影响因子 ：8.500
引用产品：CL-0456
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Prenatal dexamethasone exposure impairs rat blood-testis barrier function and sperm quality in adult offspring via GR/KDM1B/FSTL3/TGFβ signaling(2024/03/12)
作者：Yi Liu, Sijia Chen, Can Ai
期刊：ACTA PHARMACOLOGICA SINICA
DOI：10.1038/s41401-024-01244-5
影响因子 ：8.200
引用产品：CL-0456
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Human placental mesenchymal stem cells ameliorate chemotherapy-induced damage in the testis by reducing apoptosis/oxidative stress and promoting autophagy(2021/03/20)
作者：Jiafeng Lu, Zhenxing Liu, Mingkai Shu
期刊：Stem Cell Research & Therapy
DOI：10.1186/s13287-021-02275-z
影响因子 ：8.079
引用产品：CL-0456
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Aucubin, a natural iridoid glucoside, attenuates oxidative stress-induced testis injury by inhibiting JNK and CHOP activation via Nrf2 up-regulation(2019/07/27)
作者：Bo Ma, Jie Zhang, Zhiming Zhu
期刊：Phytomedicine
DOI：10.1016/j.phymed.2019.153057
影响因子 ：7.900
引用产品：CL-0456
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Toxic effects of Tripterygium glycoside tablets on the reproductive system of male rats by metabolomics, cytotoxicity, and molecular docking(2023/04/10)
作者：Jiachen Ge, Qi Qian, Yanhua Gao
期刊：PHYTOMEDICINE
DOI：10.1016/j.phymed.2023.154813
影响因子 ：7.900
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The role of ferroptosis mediated by Bmal1/Nrf2 in nicotine -induce injury of BTB integrity(2023/03/07)
作者：Zelin Zhang, Jianyong Cheng, Li Yang
期刊：FREE RADICAL BIOLOGY AND MEDICINE
DOI：10.1016/j.freeradbiomed.2023.02.024
影响因子 ：7.400
引用产品：CL-0456
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DDX58 expression promotes inflammation and growth arrest in Sertoli cells by stabilizing p65 mRNA in patients with Sertoli cell-only syndrome(2023/03/22)
作者：Hao Sun, Zhan Yang, Zhihai Teng
期刊：Frontiers in Immunology
DOI：10.3389/fimmu.2023.1135753
影响因子 ：7.300
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Chemosensitization effect of cerium oxide nanosheets by suppressing drug detoxification and efflux(2018/10/18)
作者：Guizhu Wu, Ze Zhang, Xue Chen
期刊：Ecotoxicology And Environmental Safety
DOI：10.1016/j.ecoenv.2018.10.013
影响因子 ：6.800
引用产品：CL-0456
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The rare-earth yttrium induces cell apoptosis and autophagy in the male reproductive system through ROS-Ca2+-CamkII/Ampk axis(2023/07/20)
作者：Fangpeng Hou, Junyun Huang, Furong Qing
期刊：ECOTOXICOLOGY AND ENVIRONMENTAL SAFETY
DOI：10.1016/j.ecoenv.2023.115262
影响因子 ：6.800
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Cadmium exposure induces pyroptosis of TM4 cells through oxidative stress damage and inflammasome activation(2024/01/06)
作者：Jinzhao Zhou, Yanwei Zhang, Ling Zeng
期刊：ECOTOXICOLOGY AND ENVIRONMENTAL SAFETY
DOI：10.1016/j.ecoenv.2024.115930
影响因子 ：6.800
引用产品：CL-0456
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Cascading effects of hypobaric hypoxia on the testis: insights from a single-cell RNA sequencing analysis(2023/11/15)
作者：Yunhua Ji, Linmeng Wang, Fuxun Zhang
期刊：Frontiers in Cell and Developmental Biology
DOI：10.3389/fcell.2023.1282119
影响因子 ：5.500
引用产品：CL-0456
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Aucubin supplementation alleviate diabetes induced-disruption of blood-testis barrier and testicular damage via stabilizing cell junction integrity(2022/11/29)
作者：Jingxun Wei, Xuanzhao Lu, Xiaowen Bao
期刊：EUROPEAN JOURNAL OF PHARMACOLOGY
DOI：10.1016/j.ejphar.2022.175430
影响因子 ：5.195
引用产品：CL-0456
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Non-Lethal Concentrations of CdCl2 Cause Marked Alternations in Cellular Stress Responses within Exposed Sertoli Cell Line(2023/02/09)
作者：Yonghong Man, Yunhao Liu, Chuanzhen Xiong
期刊：Toxics
影响因子 ：4.600
引用产品：CL-0456
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A Probiotic Bacillus amyloliquefaciens D-1 Strain Is Responsible for Zearalenone Detoxifying in Coix Semen(2023/11/28)
作者：Tao Deng, Yefei Chen, Jinqiang Zhang
期刊：Toxins
影响因子 ：4.200
引用产品：CL-0456
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Epigenetic programming of TBX2/CX43 mediates lower sperm quality in male offspring induced by prenatal dexamethasone exposure(2023/02/15)
作者：Yi Liu, Yi Liu, Guanghui Chen
期刊：TOXICOLOGICAL SCIENCES
影响因子 ：3.800
引用产品：CL-0456
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Polypyrimidine tract‑binding protein 1 regulates the Sertoli cell blood‑testis barrier by promoting the expression of tight junction proteins(2021/06/07)
作者：Zhe Yang, Zhi Liu, Yanping Yang
期刊：Experimental and Therapeutic Medicine
影响因子 ：2.700
引用产品：CL-0456
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